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The map label for this gene is ulaC [H]

Identifier: 15674381

GI number: 15674381

Start: 161839

End: 162324

Strand: Direct

Name: ulaC [H]

Synonym: SPy_0176

Alternate gene names: 15674381

Gene position: 161839-162324 (Clockwise)

Preceding gene: 15674380

Following gene: 15674382

Centisome position: 8.74

GC content: 42.8

Gene sequence:

>486_bases
ATGAATTTAAAACAAGCCTTTATTGACAACAACTCCATCCGATTGGGGCTAAGTGCTGATACTTGGCAAGAGGCTGTTCG
ATTAGCTGTTCAACCTTTGATTGATAGCAAAGCAGTGACGTCAGCTTACTACGACGCCATCATTGCATCAACAGAAAAAT
ACGGCCCTTATTATGTGTTGATGCCAGGTATGGCTATGCCCCATGCGGAAGCAGGTTTAGGCGTTAATCGTAATGCATTT
GCTTTGATTACACTAACAAAACCAGTCACCTTCTCAGATGGTAAAGAGGTTTCTGTTCTTTTGACGTTGGCCGCTACAGA
CCCTTCTATTCACACAACAGTGGCTATCCCACAAATTGTGGCCTTGTTTGAACTAGATAATGCCATTGAACGCTTGGTGG
CCTGCCAAAGTCCTAAAGAGGTTCTAGAGATGGTAGAAGAATCTAAAGACAGTCCTTACTTAGAAGGTATGGACTTGAAT
GCTTAA

Upstream 100 bases:

>100_bases
AGGAGTTGTGACGAAGGTTTTCAGCTCTTTCTCTTTAGTAGGATAAAACTAGTAAAATGGTGTGTGTTGCAAAGCAAGAC
ACTAGAAAGAGATAGTTTTT

Downstream 100 bases:

>100_bases
GCTTTCCTTACACAGGATACTAACGAATGATGGAAAGGATAAGAACTCTTAACCAGTTTGCACGGTGGCTAAGAGTTTTA
GGAAAAAGAGATGACACATA

Product: PTS system 3-keto-L-gulonate specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAF
ALITLTKPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
A

Sequences:

>Translated_161_residues
MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAF
ALITLTKPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
A
>Mature_161_residues
MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAF
ALITLTKPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLN
A

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=150, Percent_Identity=42.6666666666667, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790755, Length=95, Percent_Identity=38.9473684210526, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1789302, Length=106, Percent_Identity=35.8490566037736, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17475; Mature: 17475

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVL
CCCCHHEECCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEE
MPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIV
ECCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCEEEEEEEEEECCCCCEEEEHHHHHH
ALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLNA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNLKQAFIDNNSIRLGLSADTWQEAVRLAVQPLIDSKAVTSAYYDAIIASTEKYGPYYVL
CCCCHHEECCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEE
MPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLTLAATDPSIHTTVAIPQIV
ECCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCEEEEEEEEEECCCCCEEEEHHHHHH
ALFELDNAIERLVACQSPKEVLEMVEESKDSPYLEGMDLNA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]