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The map label for this gene is slo [H]

Identifier: 15674372

GI number: 15674372

Start: 151444

End: 153159

Strand: Direct

Name: slo [H]

Synonym: SPy_0167

Alternate gene names: 15674372

Gene position: 151444-153159 (Clockwise)

Preceding gene: 15674371

Following gene: 15674373

Centisome position: 8.18

GC content: 37.3

Gene sequence:

>1716_bases
ATGTCTAATAAAAAAACATTTAAAAAATACAGTCGCGTCGCTGGGCTACTGACGGCAGCTCTTATCATTGGTAACCTTGT
TACTGCTAATGCTGAATCGAACAAACAAAACACTGCTAGTACAGAAACCACAACGACAAATGAGCAACCAAAGCCAGAAA
GTAGTGAGCTAACTACTGAAAAAGCAGGTCAGAAAACGGATGATATGCTTAACTCTAACGATATGATTAAGCTTGCTCCC
AAAGAAATGCCACTAGAATCTGCAGAAAAAGAAGAAAAAAAGTCAGAAGACAAAAAAAAGAGCGAAGAAGATCACACTGA
AGAAATCAATGACAAGATTTATTCACTAAATTATAATGAGCTTGAAGTACTTGCTAAAAATGGTGAAACCATTGAAAATT
TTGTTCCTAAAGAAGGCGTTAAGAAAGCTGATAAATTTATTGTCATTGAAAGAAAGAAAAAAAATATCAACACTACACCA
GTCGATATTTCCATTATTGACTCTGTCACTGATAGGACCTATCCAGCAGCCCTTCAGCTGGCTAATAAAGGTTTTACCGA
AAACAAACCAGACGCGGTAGTCACCAAGCGAAACCCACAAAAAATCCATATTGATTTACCAGGTATGGGAGACAAAGCAA
CGGTTGAGGTCAATGACCCTACCTATGCCAATGTTTCAACAGCTATTGATAATCTTGTTAACCAATGGCATGATAATTAT
TCTGGTGGTAATACGCTTCCTGCCAGAACACAATATACTGAATCAATGGTATATTCTAAGTCACAGATTGAAGCAGCTCT
AAATGTTAATAGCAAAATCTTAGATGGTACTTTAGGCATTGATTTCAAGTCGATTTCAAAAGGTGAAAAGAAGGTGATGA
TTGCAGCATACAAGCAAATTTTTTACACCGTATCAGCAAACCTTCCTAATAATCCTGCGGATGTGTTTGATAAATCGGTG
ACCTTTAAAGAGTTGCAACGAAAAGGTGTCAGCAATGAAGCTCCGCCACTCTTTGTGAGTAACGTAGCCTATGGTCGAAC
TGTTTTTGTCAAACTAGAAACAAGTTCTAAAAGTAATGATGTTGAAGCGGCCTTTAGTGCAGCTCTAAAAGGAACAGATG
TTAAAACTAATGGAAAATATTCTGATATCTTAGAAAATAGCTCATTTACAGCTGTCGTTTTAGGAGGAGATGCTGCAGAG
CACAATAAGGTAGTCACAAAAGACTTTGATGTTATTAGAAACGTTATCAAAGACAATGCTACCTTCAGTAGAAAAAACCC
AGCTTATCCTATTTCATACACCAGTGTTTTCCTTAAAAATAATAAAATTGCGGGTGTCAATAACAGAACTGAATACGTTG
AAACAACATCTACCGAGTACACTAGTGGAAAAATTAACCTGTCTCATCAAGGCGCGTATGTTGCTCAATATGAAATCCTT
TGGGATGAAATCAATTATGATGACAAAGGAAAAGAAGTGATTACAAAACGACGTTGGGACAACAACTGGTATAGTAAGAC
ATCACCATTTAGCACAGTTATCCCACTAGGAGCTAATTCACGAAATATCCGTATCATGGCTAGAGAGTGCACTGGCTTAG
CTTGGGAATGGTGGCGAAAAGTGATCGACGAAAGAGATGTGAAACTGTCTAAAGAAATCAATGTCAATATCTCAGGATCA
ACCTTGAGCCCATATGGTTCGATTACTTATAAGTAG

Upstream 100 bases:

>100_bases
ATTACAAATTAGGAGAATTTGCTCACTATCAAGATACTATGCTTGATATGGTCGAAAGAACAATAAAAACATTTTAGAAT
GATAAAAAGGTATGAAGGAC

Downstream 100 bases:

>100_bases
GACTGGTTCAAGAGATAAGCACCTTGATGCTGCTTATCTCTTGAGGCCTTGTGCCCACTCCTCTCACAAATACAGAAAAA
AGCTTGTCTATGAGATACCT

Product: streptolysin O

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTEKAGQKTDDMLNSNDMIKLAP
KEMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNY
SGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSV
TFKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAE
HNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEIL
WDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGS
TLSPYGSITYK

Sequences:

>Translated_571_residues
MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTEKAGQKTDDMLNSNDMIKLAP
KEMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNY
SGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSV
TFKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAE
HNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEIL
WDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGS
TLSPYGSITYK
>Mature_570_residues
SNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTEKAGQKTDDMLNSNDMIKLAPK
EMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTPV
DISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNYS
GGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVT
FKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEH
NKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEILW
DEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGST
LSPYGSITYK

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 63639; Mature: 63508

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00481 THIOL_CYTOLYSINS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTE
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KAGQKTDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNE
HCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCEEEECCCCC
LEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTPVDISIIDSVTDRTYPAALQL
EEEEECCCCHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHH
ANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNY
HHCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
SGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCEEHHHEEEEE
FYTVSANLPNNPADVFDKSVTFKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSND
EEEEECCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCC
VEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEHNKVVTKDFDVIRNVIKDNA
HHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCC
TFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEIL
CCCCCCCCCCEEEEEEEEECCEEECCCCCCCEEEECCCEECCCEEEECCCCCEEEEEEEE
WDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRK
EECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHH
VIDERDVKLSKEINVNISGSTLSPYGSITYK
HHCCCCCEEEEEEEEEECCCCCCCCCCEEEC
>Mature Secondary Structure 
SNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETTTTNEQPKPESSELTTE
CCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KAGQKTDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSEEDHTEEINDKIYSLNYNE
HCCCHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCEEEECCCCC
LEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTPVDISIIDSVTDRTYPAALQL
EEEEECCCCHHHHHCCHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHHH
ANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVNDPTYANVSTAIDNLVNQWHDNY
HHCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCC
SGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDGTLGIDFKSISKGEKKVMIAAYKQI
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCEEHHHEEEEE
FYTVSANLPNNPADVFDKSVTFKELQRKGVSNEAPPLFVSNVAYGRTVFVKLETSSKSND
EEEEECCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCCEEEEEEEECCCCCCC
VEAAFSAALKGTDVKTNGKYSDILENSSFTAVVLGGDAAEHNKVVTKDFDVIRNVIKDNA
HHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCC
TFSRKNPAYPISYTSVFLKNNKIAGVNNRTEYVETTSTEYTSGKINLSHQGAYVAQYEIL
CCCCCCCCCCEEEEEEEEECCEEECCCCCCCEEEECCCEECCCEEEECCCCCEEEEEEEE
WDEINYDDKGKEVITKRRWDNNWYSKTSPFSTVIPLGANSRNIRIMARECTGLAWEWWRK
EECCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCHHHHHHH
VIDERDVKLSKEINVNISGSTLSPYGSITYK
HHCCCCCEEEEEEEEEECCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7803818 [H]