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The map label for this gene is dus1 [H]

Identifier: 15674339

GI number: 15674339

Start: 113055

End: 114032

Strand: Reverse

Name: dus1 [H]

Synonym: SPy_0122

Alternate gene names: 15674339

Gene position: 114032-113055 (Counterclockwise)

Preceding gene: 15674340

Following gene: 15674338

Centisome position: 6.16

GC content: 45.5

Gene sequence:

>978_bases
GTGATAAAGCTTAATTCTTCCTTTAGGATTGGTGATGTGGAAATCCCTCACCGCACTGTCCTTGCTCCCATGGCTGGCGT
GACCAATTCTGCCTTTCGTACCATCGCCAAAGAATTTGGTGCTGGTTTGGTGGTTATGGAAATGATTTCCGAAAAAGGGC
TTCTTTACAACAATGAAAAGACCCTTCACATGCTCCATATTGATGAAAATGAGCACCCTATGTCCATTCAATTATTTGGT
GGTGACGCAGAAGGGTTGAAACGTGCAGCTGATTTCATTCAAACCAATACCAAGGCCGATATTGTTGATATTAATATGGG
CTGCCCTGTTAATAAAGTTGTCAAAAACGAGGCTGGTGCCAAGTGGCTCCGTGACCCTGATAAGATTTACCACATTGTCA
AGGAAGTCACGTCTGTTTTAGATATTCCACTGACTGTCAAAATGCGTACAGGTTGGGCGGATAGTTCACTTGCTGTTGAA
AATGCCCTTGCCGCTGAGTCGGCAGGTGTATCTGCTCTTGCCATGCACGGTCGTACCCGTGAACAAATGTATACAGGAAC
CTGCGACCATGAGACATTAGCCCGAGTTTCTAAGGCTATCACTAAGATTCCCTTTATTGGAAATGGGGACGTGCGTTCGG
TGCAAGACGCCAAATTTATGATTGAAGAAATCGGTGTAGATGCCGTGATGATTGGCCGCGCAGCCATGAACAATCCTTAT
CTTTTTACCCAAATCAATCATTTCTTTGAAACTGGTCAAGAATTGCCAGATCTTCCCTTTGCCAAAAAATTAGACATCGC
CAAAGACCACCTTAAACGCCTGATTAACCTCAAAGGTGAAACCATTGCTGTTCGGGAATTTCGGGGCTTAGCACCACACT
ATCTTCGCGGAACAGCAGGTGCTGCCAAAGTTCGTGGTGCTGTTTCACGCGCTGAGACTCTAGCTGAGGTTGAAGCCATC
TTCGAAACAGTACGCTAA

Upstream 100 bases:

>100_bases
TTACAGGCCATGATTGACGAAGATAAGGGTGCTGAGATTGTTTGCCAATTTTGTGGTACCAAGTACCAATTTAATGAAAG
TGATTTGGAGGCCATTATCA

Downstream 100 bases:

>100_bases
TGATATTAGAAGGAGACGCATGTTGATTGTACTAGCTGGAACAATTGGGGCTGGCAAGAGCTCGCTTGCTGCTGCACTTG
GAGAGCATTTAGGAACTGAC

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFG
GDAEGLKRAADFIQTNTKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAKFMIEEIGVDAVMIGRAAMNNPY
LFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLINLKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAI
FETVR

Sequences:

>Translated_325_residues
MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFG
GDAEGLKRAADFIQTNTKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAKFMIEEIGVDAVMIGRAAMNNPY
LFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLINLKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAI
FETVR
>Mature_325_residues
MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFG
GDAEGLKRAADFIQTNTKADIVDINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAKFMIEEIGVDAVMIGRAAMNNPY
LFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLINLKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAI
FETVR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=265, Percent_Identity=32.8301886792453, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI239788462, Length=258, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI31742496, Length=293, Percent_Identity=27.3037542662116, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI40807366, Length=258, Percent_Identity=27.5193798449612, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=27.3092369477912, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=37.5776397515528, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1788462, Length=253, Percent_Identity=30.8300395256917, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI145693211, Length=249, Percent_Identity=23.6947791164659, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=235, Percent_Identity=30.6382978723404, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=251, Percent_Identity=27.4900398406374, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=27.3127753303965, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=243, Percent_Identity=24.6913580246914, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=287, Percent_Identity=24.7386759581882, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=28.8793103448276, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=287, Percent_Identity=23.3449477351916, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=33.1932773109244, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=29.8701298701299, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=29.8701298701299, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921524, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35729; Mature: 35729

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEK
CEEECCCEEECCEECCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCC
TLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIVDINMGCPVNKVVKNEAGA
EEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCC
KWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVENALAAESAGVSALAMHGRTR
HHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCCH
EQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAKFMIEEIGVDAVMIGRAAMNNPY
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHCCCCE
LFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLINLKGETIAVREFRGLAPHYLRGTAG
EEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHHCCCCC
AAKVRGAVSRAETLAEVEAIFETVR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLVVMEMISEKGLLYNNEK
CEEECCCEEECCEECCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCC
TLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIVDINMGCPVNKVVKNEAGA
EEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCC
KWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVENALAAESAGVSALAMHGRTR
HHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHCCCCH
EQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAKFMIEEIGVDAVMIGRAAMNNPY
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECHHHCCCCE
LFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLINLKGETIAVREFRGLAPHYLRGTAG
EEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCHHHCCCCC
AAKVRGAVSRAETLAEVEAIFETVR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]