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The map label for this gene is pbp1b [H]

Identifier: 15674320

GI number: 15674320

Start: 91010

End: 93322

Strand: Direct

Name: pbp1b [H]

Synonym: SPy_0097

Alternate gene names: 15674320

Gene position: 91010-93322 (Clockwise)

Preceding gene: 15674317

Following gene: 15674321

Centisome position: 4.91

GC content: 40.25

Gene sequence:

>2313_bases
ATGAGGTATTTTATGGTGAAATGGAACACGAAACAAAAGCGTATAAGTCATCAAAGATTAGGTCTCTTGGATTTGGGGCC
GGTGCTATTACGTACGTTGAGACTACTGTCTAACTTTTTTTATATTGTTATCTTCCTTTTTGGAATGATGGGATTTGGTA
TGGCATTTGGGTATTTGGCTAGTCAGATTGAATCTGTTAAGGTACCAAGTAAAGAAAGTTTAGTCAAACAAGTTGAATCA
TTAACGATGATTTCGCAAATGAACTATTCTGATAATAGTTTAATTTCTACTTTAGATACGGATTTACTTCGAACACCAGT
AGCTAATGATGCGATTTCAGAGAATATCAAAAAAGCTATTGTATCAACAGAAGACGAACATTTTCAAGAACATAAAGGTA
TCGTGCCAAAAGCTGTTTTTCGGGCAACATTGGCTTCTGTATTGGGATTTGGAGAAGCTAGTGGAGGTTCGACCTTAACA
CAGCAATTAGTCAAGCAACAAGTTTTGGGAGACGATCCCACATTTAAGCGCAAGTCTAAGGAGATCGTTTACGCTCTTGC
CTTAGAGCGTTATATGTCCAAAGACAATATCTTATGTGATTATCTTAATGTTTCACCTTTTGGCCGTAACAACAAGGGTC
AAAATATTGCTGGTGTTGAAGAAGCTGCGCGTGGCATTTTTGGCGTTTCTGCCAAAGATTTAACGGTGCCACAGGCAGCA
TTTTTGGCGGGTCTTCCGCAGAGTCCTATTGTTTACTCTCCTTATTTGTCAACGGGACAACTGAAATCAGAAAAGGACAT
GGCTTATGGCATCAAGCGTCAGCAAAATGTTCTCTTTAACATGTACCGTACAGGTGTTCTGTCTAAAAAAGAATACGAGG
ACTATAAGGCTTATCCGATTCAAAAGGATTTTATTCAACCGGGAAGTGCAATAGTAAATAATCACGATTACCTTTATTAC
ACGGTGTTAGCGGATGCTAAGAAAGCCATGTATAGCTATTTGATTAAGCGAGATAAGGTGTCTAGTCGTGACTTGAAAAA
TGACGAGACTAAGGCTGCTTATGAAGAGAGAGCCTTAACAGAATTGCAACAGGGTGGCTATACCATCACCACAACCATTA
ATAAGCCTATTTACAATGCGATGCAGACAGCGGCAGCTCAGTTTGGTGGCTTGTTAGATGATGGCACTGGTACAGTTCAA
ATGGGAAATGTCTTGACAGACAATGCGACTGGTGCTGTGTTAGGTTTTGTTGGTGGTAGAGATTATGCTCTGAATCAAAA
TAATCATGCTTTCAATACAGTTAGATCGCCAGGTTCTAGCATTAAACCGATAATCGCTTATGGTCCTGCTATTGATCAAG
GTTTAATGGGGAGTGCTAGCGTTTTGTCTAATTACCCAACAACTTACTCGAGTGGCCAAAAAATCATGCATGCTGATAGT
GAAGGAACAGCCATGATGCCACTTCAAGAGGCCCTAAATACTTCTTGGAACATCCCAGCTTTTTGGACACAGAAATTACT
GCGTGAAAAAGGTGTCGATGTCGAAAATTATATGACAAAAATGGGTTATAAGATTGCAGACTACTCGATTGAAAGTTTAC
CTCTAGGGGGCGGTATTGAAGTCTCGGTTGCTCAACAAACCAATGCTTACCAAATGCTTTCAAACAATGGCTTATATCAA
AAGCAATATATTGTAGATAAGATTACTGCTAGCGATGGTACAGTCGTTTACAAACATGAAAATAAGCCAATTCGTATTTT
TTCTGCAGCAACAGCTACGATTTTACAAGAATTGTTGAGAGGTCCGATTACTTCAGGCGCTACGACTACTTTCAAGAACC
GTTTGGCGGCTATTAATCCGTGGCTTGCTAATGCTGATTGGATTGGTAAGACCGGAACAACTGAGAATTATACGGATGTT
TGGCTAGTCCTGTCTACTCCAAAAGTTACTTTAGGCGGTTGGGCAGGACATGATGACAATACCTCATTAGCGCCATTAAC
AGGATATAACAATAATTCTAATTATCTTGCCTATTTAGCTAATGCCATTAATCAGGCCGATCCCAATGTTATTGGAGTAG
GGCAACGCTTCAACTTAGATCCAGGAGTCATTAAGGCGAATGTCTTGAAGTCAACAGGTTTACAACCAGGAACTGTTAAT
GTCAATGGACATACTTTTTCTGTTGGTGGAGAAATGACCACCAGTCTATGGTCCCAAAAAGGACCGGGGGCTATGACTTA
CCGATTTGCTATTGGTGGCACGGATGCCGATTATCAAAAAGCCTGGGGGAACTTCGGGTTCAGAAAAAATTAG

Upstream 100 bases:

>100_bases
AATATTCATAGGCTTTCTCCATTTTCATTATAGTTTCGAATCATTATATCAAAAAACGCTACTGCTATGCAAGCTTTATC
AAAGTTTGGTATAATGATAA

Downstream 100 bases:

>100_bases
GTTGCCATTTATCGATAAAAAGGGTATAATAGTAATAACTATTTGTCGTCCAATCTAGCTGAAATATTGTCCAGTTAGGA
AGAACAGCAGTTAAATCACA

Product: putative penicillin-binding protein 1b

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVES
LTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLT
QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAA
FLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYY
TVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQ
MGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADS
EGTAMMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQ
KQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDV
WLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVN
VNGHTFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN

Sequences:

>Translated_770_residues
MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVES
LTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLT
QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAA
FLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYY
TVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQ
MGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADS
EGTAMMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQ
KQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDV
WLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVN
VNGHTFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
>Mature_770_residues
MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVES
LTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAIVSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLT
QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAA
FLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYY
TVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQ
MGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADS
EGTAMMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQ
KQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDV
WLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVN
VNGHTFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=616, Percent_Identity=25.6493506493506, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI87082258, Length=349, Percent_Identity=28.3667621776504, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1786343, Length=577, Percent_Identity=25.3032928942808, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1789601, Length=179, Percent_Identity=31.2849162011173, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 84499; Mature: 84499

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLA
CEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAI
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLTQQLVKQQVLGDDPTFKRKSK
HCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHH
FLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFNMYRTGVLSKKEYEDYKAYPI
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
QKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALT
CHHHCCCCCCEECCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNVLTDNATGAVLGFVGGR
HHHHCCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCEEEECCEEECCCCCEEEEEECCC
DYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADS
CEEECCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCC
EGTAMMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIE
CCCEECCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHCCCCCCCEE
VSVAQQTNAYQMLSNNGLYQKQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLR
EEEECCCCHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHC
GPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDN
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCC
TSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVN
CCCCEEECCCCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCEEEHHHHHCCCCCCEEE
VNGHTFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
ECCEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVIFLFGMMGFGMAFGYLA
CEEEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDLLRTPVANDAISENIKKAI
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VSTEDEHFQEHKGIVPKAVFRATLASVLGFGEASGGSTLTQQLVKQQVLGDDPTFKRKSK
HCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
EIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAGVEEAARGIFGVSAKDLTVPQAA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHH
FLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQNVLFNMYRTGVLSKKEYEDYKAYPI
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCC
QKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYLIKRDKVSSRDLKNDETKAAYEERALT
CHHHCCCCCCEECCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ELQQGGYTITTTINKPIYNAMQTAAAQFGGLLDDGTGTVQMGNVLTDNATGAVLGFVGGR
HHHHCCEEEEEECCCHHHHHHHHHHHHHCCEEECCCCEEEECCEEECCCCCEEEEEECCC
DYALNQNNHAFNTVRSPGSSIKPIIAYGPAIDQGLMGSASVLSNYPTTYSSGQKIMHADS
CEEECCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCC
EGTAMMPLQEALNTSWNIPAFWTQKLLREKGVDVENYMTKMGYKIADYSIESLPLGGGIE
CCCEECCHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHCCCCCCCEE
VSVAQQTNAYQMLSNNGLYQKQYIVDKITASDGTVVYKHENKPIRIFSAATATILQELLR
EEEECCCCHHHHHHCCCCCHHHHHHHEEECCCCEEEEEECCCCEEEEHHHHHHHHHHHHC
GPITSGATTTFKNRLAAINPWLANADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDN
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCC
TSLAPLTGYNNNSNYLAYLANAINQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVN
CCCCEEECCCCCCCHHHHHHHHHCCCCCCEEECCCEECCCCCCEEEHHHHHCCCCCCEEE
VNGHTFSVGGEMTTSLWSQKGPGAMTYRFAIGGTDADYQKAWGNFGFRKN
ECCEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]