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The map label for this gene is tyrS [H]

Identifier: 15674319

GI number: 15674319

Start: 89662

End: 90918

Strand: Reverse

Name: tyrS [H]

Synonym: SPy_0096

Alternate gene names: 15674319

Gene position: 90918-89662 (Counterclockwise)

Preceding gene: 15674334

Following gene: 15674318

Centisome position: 4.91

GC content: 41.45

Gene sequence:

>1257_bases
ATGAATATTTTTGAAGAACTCAAAGCTCGTGGCTTGGTCTTTCAAACGACTGATGAACAAGCCCTTGTCAAAGCATTAAC
AGAAGGGCAAGTATCCTATTATACCGGTTATGATCCAACCGCTGACAGCCTTCATCTGGGCCATCTAGTGGCTATCTTAA
CATCTCGTCGCTTGCAACTAGCAGGGCACAAACCTTACGCTCTTGTGGGGGGTGCCACAGGTTTAATTGGTGATCCTTCC
TTTAAAGATGCAGAGCGCAGCCTTCAAACCAAAGAAACAGTTTTGGAGTGGAGTGACAAGATTAAAGGGCAGTTGTCTAC
TTTCCTTGATTTTGAAAATGGTGATAATAAAGCAGAGCTTGTCAACAACTACGACTGGTTCTCGCAAATCAGCTTTATTG
ACTTCCTCCGTGATGTCGGTAAATACTTCACCGTTAACTACATGATGAGTAAAGACTCTGTTAAAAAACGCATTGAAACA
GGCATTTCTTACACTGAGTTTGCTTACCAAATCATGCAGGGCTACGACTTCTACGAACTCAATGACAAGCATAATGTAAC
CTTACAAATTGGCGGCTCTGACCAGTGGGGTAATATGACAGCTGGTACTGAATTGCTCCGCAAAAAGGCTGATAAAACTG
GGCACGTCATGACTGTACCACTCATCACTGACTCAACCGGGAAAAAATTCGGTAAATCAGAAGGTAATGCTGTCTGGCTT
GATGCCGATAAGACGTCTCCTTACGAGATGTACCAATTCTGGTTAAATGTCATGGATGATGATGCAGTGCGTTTCTTGAA
AATCTTCACCTTCTTATCTCTAGATGAGATTGCAGAAATTGAAACTCAATTTAATGCCGCTCGTCACGAGCGTCTCGCTC
AAAAAACCTTGGCACGCGAAGTGGTTACCTTGGTTCATGGCGAAGAAGCTTATAAACAAGCTCTTAACATTACCGAGCAG
TTATTTGCTGGAAACATCAAAAATCTTTCTGCCAATGAATTAAAACAAGGATTGAGCAATGTACCAAATTATCACGTTCA
ATCAATAGACAATCATAATATTGTCGAGATTTTAGTAGCTGCTAAGATTTCCCCATCAAAACGCCAGGCGCGTGAAGACG
TGCAAAATGGAGCAATCTACATTAACGGAGACCGCGTTCAAGATTTAGATTACCAATTAAGTAATGATGATAAAATTGAT
GATCAATTAACCGTTATTCGCCGCGGTAAGAAAAAATACGCTGTTCTCACTTACTAG

Upstream 100 bases:

>100_bases
ATACCTCATTTATCATTATACCAAACTTTGATAAAGCTTGCATAGCAGTAGCGTTTTTTGATATAATGATTCGAAACTAT
AATGAAAATGGAGAAAGCCT

Downstream 100 bases:

>100_bases
ATAACCGATTAAACGAGCTCTGGCTCGTTTTTTCATAAGGAGATGTACCTATGACAGAATGTATCTTTTGCCATCAGCTC
AAAGCAAATCAGCTACTGAC

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY

Sequences:

>Translated_418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY
>Mature_418_residues
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPS
FKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQ
LFAGNIKNLSANELKQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQLSNDDKID
DQLTVIRRGKKKYAVLTY

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=432, Percent_Identity=35.8796296296296, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1787925, Length=421, Percent_Identity=49.8812351543943, Blast_Score=438, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI25145802, Length=396, Percent_Identity=33.5858585858586, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25145805, Length=332, Percent_Identity=31.6265060240964, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6325160, Length=442, Percent_Identity=34.841628959276, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI20130383, Length=435, Percent_Identity=39.0804597701149, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47342; Mature: 47342

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAEL
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
VNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIETGISYTEFAYQIMQGYDFYEL
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
NDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIETQFNAARHERLAQKTLARE
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTLVHGEEAYKQALNITEQLFAGNIKNLSANELKQGLSNVPNYHVQSIDNHNIVEILVA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEEE
AKISPSKRQAREDVQNGAIYINGDRVQDLDYQLSNDDKIDDQLTVIRRGKKKYAVLTY
CCCCCHHHHHHHHHHCCEEEEECCEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSLHLGHLVAILTSRRLQL
CCHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHEE
AGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIKGQLSTFLDFENGDNKAEL
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHH
VNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIETGISYTEFAYQIMQGYDFYEL
CCCCHHHHHHHHHHHHHHHHHHEEEEEEECHHHHHHHHHHCCCHHHHHHHHHHCCCEEEE
NDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMTVPLITDSTGKKFGKSEGNAVWL
CCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEE
DADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDEIAEIETQFNAARHERLAQKTLARE
ECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVTLVHGEEAYKQALNITEQLFAGNIKNLSANELKQGLSNVPNYHVQSIDNHNIVEILVA
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCEEEEEE
AKISPSKRQAREDVQNGAIYINGDRVQDLDYQLSNDDKIDDQLTVIRRGKKKYAVLTY
CCCCCHHHHHHHHHHCCEEEEECCEEECCCCCCCCCCCCHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA