Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 15674272

GI number: 15674272

Start: 42780

End: 43334

Strand: Direct

Name: purN [H]

Synonym: SPy_0028

Alternate gene names: 15674272

Gene position: 42780-43334 (Clockwise)

Preceding gene: 15674271

Following gene: 15674274

Centisome position: 2.31

GC content: 46.49

Gene sequence:

>555_bases
ATGAAAATCGCTGTTTTTGCTTCTGGTAATGGTTCCAACTTTCAGGTCATAGCAGAGCAGTTTCCAGTTAGTTTTGTCTT
TTCAGATCATCGTGATGCCTATGTTTTAGAGCGTGCTCAGAATTTAGCCATTCCAAGCTTCGCTTTTGAACTCAAGGAGT
TTGAGAATAAGGTGGCTTACGAGCAAGCCATCGTTGATTTGTTGGACAAACACGAGATTGACTTAGTCTGTCTGGCAGGC
TATATGAAGATTGTTGGAGAAACCTTACTCTTGGCCTATGAGAGGCGTATTATCAATATTCACCCAGCCTACCTGCCTGA
ATTTCCAGGTGCCCACGGTATCGAGGATGCTTGGGAAGCTGGTGTTGACCAGTCTGGCGTGACCATCCACTGGGTTGACT
CTGGTGTGGATACCGGTCAGGTCATCCAACAAGTGCGCGTGCCACGCCTAGCAGACGATAGCCTAGAAAGCTTTGAAACC
CGTATCCATGAAACCGAATATCAACTCTACCCAGCTGTCTTAGATAGCTTGGGAGTGGAGAGGAAAGTAATCTAG

Upstream 100 bases:

>100_bases
AGGAAAAAAGATAAAATGACTTAGACGCAAGCGTCGTTGTTCGTTTTCCTATTTTTCTACGAAATTTTTCGGCAAGCCGA
ATCATCCCTTTGTATCGAAT

Downstream 100 bases:

>100_bases
CCTTTATCTTAAAATTTTAGGTAAAGTTAGGAAAGATAGTAAAGAAGAATTAACGTTCCAAAAAGAAAGGAATTGAACCC
GTCTGGAAGGCACAGAAAAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKVAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYERRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI

Sequences:

>Translated_184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKVAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYERRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI
>Mature_184_residues
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKVAYEQAIVDLLDKHEIDLVCLAG
YMKIVGETLLLAYERRIINIHPAYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
RIHETEYQLYPAVLDSLGVERKVI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=180, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI209869995, Length=180, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=1e-27,
Organism=Homo sapiens, GI209869993, Length=180, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=35.9116022099448, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787483, Length=98, Percent_Identity=35.7142857142857, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=32.0441988950276, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=28.2828282828283, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24582400, Length=185, Percent_Identity=36.7567567567568, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 20682; Mature: 20682

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKVAY
CEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYERRIINIHPAYLPEFPGAHGIEDAWEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHC
GVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFETRIHETEYQLYPAVLDSLGVE
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RKVI
CCCC
>Mature Secondary Structure
MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAIPSFAFELKEFENKVAY
CEEEEEECCCCCCEEEEHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQAIVDLLDKHEIDLVCLAGYMKIVGETLLLAYERRIINIHPAYLPEFPGAHGIEDAWEA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCHHHHC
GVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFETRIHETEYQLYPAVLDSLGVE
CCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RKVI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]