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The map label for this gene is purF [H]

Identifier: 15674270

GI number: 15674270

Start: 40108

End: 41562

Strand: Direct

Name: purF [H]

Synonym: SPy_0026

Alternate gene names: 15674270

Gene position: 40108-41562 (Clockwise)

Preceding gene: 15674269

Following gene: 15674271

Centisome position: 2.17

GC content: 39.11

Gene sequence:

>1455_bases
ATGACATACGAAGTAAAATCTCTAAATGAAGAATGTGGAGTCTTTGGTATCTGGGGACATCCTCAGGCAGCTCAAGTCAC
TTACTTTGGGCTTCATAGCCTTCAACATCGCGGTCAAGAAGGAGCTGGTATTGTTTCAAATGACAATGGGAAACTCTATG
GTTATCGAAATGTTGGGCTCCTTTCTGAAGTTTTTAAGAATCAATCTGAATTAGATAATTTAACTGGGAATGCGGCTATT
GGACATGTTCGGTATGCTACTGCAGGTTCTGCAGATATTCGCAATATTCAGCCTTTTCTTTATAAATTTCATGACGGGCA
ATTTGCTTTATGCCATAATGGTAATTTGACAAATGCTATTTCCTTAAGGAAAGAATTAGAAAAGCAAGGTGCAATTTTCA
ATGCCTCCTCAGATACTGAAATTTTGATGCACTTGATTCGTCGAAGCCATAACTCAAGTTTCATGGGAAAGGTAAAAGAA
GCTCTAAATACAGTAAAGGGTGGCTTTGCCTATCTACTGATGACAGAAAATAAACTCATTGCTGCTCTTGACCCTAATGC
CTTTCGTCCTTTGTCAATTGGACAAATGCAAAATGGTGCCTGGGTTATTTCCAGTGAGACCTGTGCTTTTGAGGTGGTAG
GCGCAAAATGGGTTAGAGATGTTGAACCTGGCGAAGTTATTCTTATAGACGATCGTGGTATTCAATGTGATCGTTATACT
GATGAAACGCAACTGGCAATTTGTTCGATGGAATATGTCTATTTTGCAAGGCCGGATTCAACTATACATGGTGTTAATGT
TCATACGGCTCGAAAAAATATGGGAAAGCGTCTTGCACAAGAATTTAAACAGGATGCTGATATTGTAATTGGTGTCCCAA
ATTCGTCCTTATCGGCTGCTATGGGCTTTGCTGAAGAATCCGGATTACCAAATGAGATGGGTCTTGTAAAAAATCAGTAT
ACGCAGCGAACCTTTATTCAACCGACACAAGAATTAAGGGAACAAGGTGTTCGAATGAAACTATCAGCGGTATCCGGTGT
TGTCAAAGGAAAGCGCGTTGTTATGATTGATGACTCAATTGTAAGAGGAACGACTTCTAGAAGGATTGTCGGATTATTAA
GAGAAGCAGGAGCTAGTGAAGTACATGTTGCTATAGCTAGTCCAGAATTAAAGTATCCTTGTTTTTATGGTATTGATATT
CAGACTCGTCGTGAGTTGATTTCAGCCAATCACTCTGTTGATGAAGTGTGTGATATTATTGGCGCAGATAGTCTGACCTA
TCTCTCACTGGATGGGTTGATTGAATCAATTGGACTCGAAACAAAAGCACCAAATGGTGGTTTATGCGTAGCTTACTTTG
ATGGGCATTATCCAACACCGCTTTATGATTATGAAGAAGAATACCTCAGAAGTTTAGAAGAGAAAACAAGTTTTTATATT
CAAAAAGTGAAATAA

Upstream 100 bases:

>100_bases
AATCTCGCTTAATTTTTGAGAAATAGTAAACACGGGCGATAAGTTGTGCGAAAAACTTTCTATTTTCGCTTTACTTGACA
GCCTTTGTATCGAGGTAAAA

Downstream 100 bases:

>100_bases
GCTCTGCTAATTATATAAGGAGAAAACATGTCTGAAAAAAATGCTTATGCAAAATCTGGTGTTGATGTTGAAGCTGGTTA
TGAAGTGGTTGAAAGAATAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGIVSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAI
GHVRYATAGSADIRNIQPFLYKFHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWVRDVEPGEVILIDDRGIQCDRYT
DETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGKRLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKYPCFYGIDI
QTRRELISANHSVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCVAYFDGHYPTPLYDYEEEYLRSLEEKTSFYI
QKVK

Sequences:

>Translated_484_residues
MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGIVSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAI
GHVRYATAGSADIRNIQPFLYKFHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWVRDVEPGEVILIDDRGIQCDRYT
DETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGKRLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQY
TQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKYPCFYGIDI
QTRRELISANHSVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCVAYFDGHYPTPLYDYEEEYLRSLEEKTSFYI
QKVK
>Mature_483_residues
TYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGIVSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIG
HVRYATAGSADIRNIQPFLYKFHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKEA
LNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWVRDVEPGEVILIDDRGIQCDRYTD
ETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGKRLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYT
QRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKYPCFYGIDIQ
TRRELISANHSVDEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCVAYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQ
KVK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=516, Percent_Identity=37.2093023255814, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1788651, Length=485, Percent_Identity=39.3814432989691, Blast_Score=307, Evalue=8e-85,
Organism=Escherichia coli, GI1790167, Length=226, Percent_Identity=26.1061946902655, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=38.8308977035491, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6323958, Length=497, Percent_Identity=34.6076458752515, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24659598, Length=484, Percent_Identity=39.4628099173554, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24659604, Length=460, Percent_Identity=39.7826086956522, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=39.8797595190381, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53561; Mature: 53430

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGIVSNDNGKLYGYRNVGL
CCEECCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCEEEEECCHH
LSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYKFHDGQFALCHNGNLTNAI
HHHHHCCHHHHHHCCCCCEECEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCHHH
SLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKEALNTVKGGFAYLLMTENKLI
HHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEE
AALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWVRDVEPGEVILIDDRGIQCDRYT
EEECCCCCCCCCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCEEEEECCCCCCCCCC
DETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGKRLAQEFKQDADIVIGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCEEECEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEESGLPNEMGLVKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMIDDSI
HCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVGLLREAGASEVHVAIASPELKYPCFYGIDIQTRRELISANHSVDEVCDII
HCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHH
GADSLTYLSLDGLIESIGLETKAPNGGLCVAYFDGHYPTPLYDYEEEYLRSLEEKTSFYI
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEE
QKVK
EECC
>Mature Secondary Structure 
TYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGIVSNDNGKLYGYRNVGL
CEECCCCCCCCCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCEEECCCCCEEEEECCHH
LSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYKFHDGQFALCHNGNLTNAI
HHHHHCCHHHHHHCCCCCEECEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCHHH
SLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKEALNTVKGGFAYLLMTENKLI
HHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCEE
AALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWVRDVEPGEVILIDDRGIQCDRYT
EEECCCCCCCCCCCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCEEEEECCCCCCCCCC
DETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGKRLAQEFKQDADIVIGVPNSSLSAA
CCCEEEEEEEEEEEEECCCCEEECEEEEHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHH
MGFAEESGLPNEMGLVKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMIDDSI
HCCHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEHHHHHHHCCCEEEEECCHH
VRGTTSRRIVGLLREAGASEVHVAIASPELKYPCFYGIDIQTRRELISANHSVDEVCDII
HCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHHH
GADSLTYLSLDGLIESIGLETKAPNGGLCVAYFDGHYPTPLYDYEEEYLRSLEEKTSFYI
CCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEE
QKVK
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA