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The map label for this gene is prsA.2 [H]

Identifier: 15674264

GI number: 15674264

Start: 31995

End: 32957

Strand: Direct

Name: prsA.2 [H]

Synonym: SPy_0020

Alternate gene names: 15674264

Gene position: 31995-32957 (Clockwise)

Preceding gene: 15674263

Following gene: 15674265

Centisome position: 1.73

GC content: 39.04

Gene sequence:

>963_bases
ATGTCTTATTCTGATTTAAAATTGTTTGCTTTATCGTCAAACAAGGAATTAGCAGAAAAAGTTGCGTCTGCAATGGGTAT
TCAACTTGGAAAGTCTACGGTTCGCCAGTTTTCAGATGGTGAAATCCAAGTAAATATTGAAGAATCAATTCGTGGTCATC
ATGTCTTTATTTTGCAATCAACTAGCTCACCTGTCAATGATAATTTAATGGAAATTTTGATTATGGTCGATGCTTTAAAG
CGTGCTAGTGCTGAAAAAATTTCAGTTGTGATGCCTTACTACGGCTATGCACGCCAAGACCGTAAAGCGCGTTCTCGTGA
ACCTATTACTTCAAAATTAGTTGCTAATATGTTAGAAGTTGCTGGTGTAGATCGCTTATTAACGGTTGATTTACATGCTG
CTCAAATTCAAGGGTTCTTTGATATTCCAGTTGATCACTTGATGGGTGCACCTTTAATTGCAGACTATTTTGATCGTCAT
GGTTTAGTGGGTGAAGATGTTGTCGTTGTTAGTCCTGACCATGGTGGTGTGACTCGTGCGCGTAAATTAGCACAATTCCT
CCAAACACCAATTGCAATTATCGATAAGCGTCGTAGTGTGGATAAGATGAACACCAGTGAAGTGATGAATATTATCGGAA
ATGTTTCGGGGAAGAAATGTATCTTGATTGACGATATGATTGATACCGCAGGGACTATTTGCCATGCAGCAGATGCGCTT
GCTGAAGCGGGGGCAACAGCTGTGTATGCGTCATGTACGCATCCCGTTCTTTCTGGACCAGCACTTGATAATATTCAACG
TTCGGCTATCGAAAAACTAATTGTTCTTGATACTATTTATCTGCCAAAAGAACGCCTTATTGATAAAATTGAACAAATTT
CAATTGCTGATTTGGTTGCAGAAGCGATTATTCGTATTCATGAAAAACGTCCGTTGTCACCTTTATTTGAGATGGGCAAT
TAA

Upstream 100 bases:

>100_bases
AAGGTATATTTAATACATTCTTTAACGGTTTTTCAATTTCATTTGATAATTCACTTGAAAAGCGTTAAAATGGTATAGGA
TTAAAACTTTGGAGGAAAAA

Downstream 100 bases:

>100_bases
ACTTAACGAAGCCTGAGATAATAGATCTCGGGCTTTTTGACTTGAATAAAATGTAAGGGTAATGTGGTATTAATTATTGT
GGGATGAACTATGCAATTCT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 1; Phosphoribosyl pyrophosphate synthase 1; P-Rib-PP synthase 1; PRPP synthase 1 [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQVNIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALK
RASAEKISVVMPYYGYARQDRKARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKCILIDDMIDTAGTICHAADAL
AEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN

Sequences:

>Translated_320_residues
MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQVNIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALK
RASAEKISVVMPYYGYARQDRKARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKCILIDDMIDTAGTICHAADAL
AEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN
>Mature_319_residues
SYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQVNIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKR
ASAEKISVVMPYYGYARQDRKARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRHG
LVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKCILIDDMIDTAGTICHAADALA
EAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=315, Percent_Identity=46.3492063492063, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=46.3492063492063, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI84875539, Length=318, Percent_Identity=45.9119496855346, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI28557709, Length=313, Percent_Identity=44.7284345047923, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI4506133, Length=344, Percent_Identity=36.6279069767442, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI194018537, Length=345, Percent_Identity=33.3333333333333, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI310128524, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310115209, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310118259, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI310119946, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787458, Length=316, Percent_Identity=51.5822784810127, Blast_Score=327, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI25149168, Length=315, Percent_Identity=45.3968253968254, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17554702, Length=315, Percent_Identity=45.3968253968254, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71989924, Length=315, Percent_Identity=45.3968253968254, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=45.5128205128205, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17570245, Length=339, Percent_Identity=33.0383480825959, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320946, Length=318, Percent_Identity=43.0817610062893, Blast_Score=248, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6321776, Length=321, Percent_Identity=42.0560747663551, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319403, Length=318, Percent_Identity=42.1383647798742, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=41.1167512690355, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=114, Percent_Identity=42.1052631578947, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=46.031746031746, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=43.1952662721894, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI281362873, Length=357, Percent_Identity=34.453781512605, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24651454, Length=357, Percent_Identity=34.453781512605, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24651458, Length=357, Percent_Identity=34.453781512605, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24651456, Length=357, Percent_Identity=34.453781512605, Blast_Score=180, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24651462, Length=376, Percent_Identity=32.7127659574468, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651464, Length=376, Percent_Identity=32.7127659574468, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45552010, Length=376, Percent_Identity=32.7127659574468, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35044; Mature: 34913

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQVNIEESIRGHHVFILQS
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCEEEEEEC
TSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRKARSREPITSKLVANMLEV
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHH
AGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRHGLVGEDVVVVSPDHGGVTRA
HCCCEEEEEEEHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKCILIDDMIDTAGTICHAADAL
HHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHH
AEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDTIYLPKERLIDKIEQISIADLVA
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAIIRIHEKRPLSPLFEMGN
HHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
SYSDLKLFALSSNKELAEKVASAMGIQLGKSTVRQFSDGEIQVNIEESIRGHHVFILQS
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCEEEEEEC
TSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRKARSREPITSKLVANMLEV
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHH
AGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRHGLVGEDVVVVSPDHGGVTRA
HCCCEEEEEEEHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHH
RKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGKKCILIDDMIDTAGTICHAADAL
HHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHH
AEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVLDTIYLPKERLIDKIEQISIADLVA
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAIIRIHEKRPLSPLFEMGN
HHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12354221 [H]