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The map label for this gene is fmt

Identifier: 15673875

GI number: 15673875

Start: 1961034

End: 1961993

Strand: Reverse

Name: fmt

Synonym: L0362

Alternate gene names: 15673875

Gene position: 1961993-1961034 (Counterclockwise)

Preceding gene: 30024060

Following gene: 15673874

Centisome position: 82.94

GC content: 38.44

Gene sequence:

>960_bases
ATGACAAAAACAAAAATTATCTTTATGGGGACACCACAATTTGCTGCGACCGTTTTAAAAGGTTTGATTGATTCTAATCA
ATATGAAATTCTAGCAGTAGTGACTCAACCAGACCGTAAGGTTGGTCGGAAACAGGAATTAAGAATGACACCAGTTAAAG
AACTTGCCTTAACGGTTAACTTGCCTGTTCTTCAACCAGAAAAACTTTCAGGCTCAGTAGAAATGACTCAAATCATGACG
CTCCTTGAATCAGGAGAAGTTGGAATTGTGACTGCCGCCTTTGGACAATTTTTGCCAGGTAAATTATTAGATGTTGCTCA
ATTTGCAGTCAATACTCATGCTTCTTTACTACCAAAATATCGCGGTGGGGCACCTATTCATTATGCTATTATGAATGGCG
AAAAAGAAGCTGGTGTAACCATCATGGAAATGATTCGTAAAATGGATGCCGGTGACATGATTGCTCAAGATTCTACTCCA
ATTCTTGAGGATGATAATGTTGGCACTATGTTTGAAAAGTTAGCCCTTGTTGGACGTGATTTGTTACTTGAAACATTGCC
AAAATACCTGAGTGGCCAATTAAAAGCTCAAGCTCAAAACGAAGATGAAGTGACTTTTAGTCCTAATATTTCGCCAGAAG
AAGAAAAAATCGACTGGAATAAATCTGCACGTGAAATTTTCAATAAAGTTCGTGGTATGAATCCATTCCCAGTTGCACAT
ACCACATGGAATGGCGAGCGTTTCAAGATTTATGAAACAAAAGTTGTTGATGATTCTGTCGGTAACTTACAAGCTGGTGA
AATTGTGGAAAAAACGAAGAAATCATTAAAAGTTGCCACAGGTGAGGGACTCCTTGAACTTCTTTTCGTTCAACCAGCAG
GTAAACCCAAGATGGATATCGTTAGCTTTCTTAACGGTCTAGGTCAAAAAATACAAGTTGGAGATAAGTTTGGCGACTAA

Upstream 100 bases:

>100_bases
TTATTCCAGAAAATCTTGGACAGTTAATGGCAAAAGGCGATAAGGCTGTTCAAGTATTAATGAAACAAAAGAAAATTATT
ATCGAGGAGTTTGAAAACTT

Downstream 100 bases:

>100_bases
ATTTATCCACAATAATAAATTAAAAGTTCTATCATTTTTAATGATAGAACTTTTTTTACATCAAAATATGGTAAATAAGT
TAGAGTGTAATAAAAAGAAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTKTKIIFMGTPQFAATVLKGLIDSNQYEILAVVTQPDRKVGRKQELRMTPVKELALTVNLPVLQPEKLSGSVEMTQIMT
LLESGEVGIVTAAFGQFLPGKLLDVAQFAVNTHASLLPKYRGGAPIHYAIMNGEKEAGVTIMEMIRKMDAGDMIAQDSTP
ILEDDNVGTMFEKLALVGRDLLLETLPKYLSGQLKAQAQNEDEVTFSPNISPEEEKIDWNKSAREIFNKVRGMNPFPVAH
TTWNGERFKIYETKVVDDSVGNLQAGEIVEKTKKSLKVATGEGLLELLFVQPAGKPKMDIVSFLNGLGQKIQVGDKFGD

Sequences:

>Translated_319_residues
MTKTKIIFMGTPQFAATVLKGLIDSNQYEILAVVTQPDRKVGRKQELRMTPVKELALTVNLPVLQPEKLSGSVEMTQIMT
LLESGEVGIVTAAFGQFLPGKLLDVAQFAVNTHASLLPKYRGGAPIHYAIMNGEKEAGVTIMEMIRKMDAGDMIAQDSTP
ILEDDNVGTMFEKLALVGRDLLLETLPKYLSGQLKAQAQNEDEVTFSPNISPEEEKIDWNKSAREIFNKVRGMNPFPVAH
TTWNGERFKIYETKVVDDSVGNLQAGEIVEKTKKSLKVATGEGLLELLFVQPAGKPKMDIVSFLNGLGQKIQVGDKFGD
>Mature_318_residues
TKTKIIFMGTPQFAATVLKGLIDSNQYEILAVVTQPDRKVGRKQELRMTPVKELALTVNLPVLQPEKLSGSVEMTQIMTL
LESGEVGIVTAAFGQFLPGKLLDVAQFAVNTHASLLPKYRGGAPIHYAIMNGEKEAGVTIMEMIRKMDAGDMIAQDSTPI
LEDDNVGTMFEKLALVGRDLLLETLPKYLSGQLKAQAQNEDEVTFSPNISPEEEKIDWNKSAREIFNKVRGMNPFPVAHT
TWNGERFKIYETKVVDDSVGNLQAGEIVEKTKKSLKVATGEGLLELLFVQPAGKPKMDIVSFLNGLGQKIQVGDKFGD

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=269, Percent_Identity=27.5092936802974, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI238814322, Length=260, Percent_Identity=23.4615384615385, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI21614513, Length=160, Percent_Identity=25.625, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1789683, Length=314, Percent_Identity=38.5350318471338, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788589, Length=263, Percent_Identity=31.5589353612167, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI133930964, Length=263, Percent_Identity=27.7566539923954, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319458, Length=274, Percent_Identity=25.9124087591241, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI45550868, Length=312, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571984, Length=224, Percent_Identity=31.6964285714286, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24585660, Length=245, Percent_Identity=26.1224489795918, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 35081; Mature: 34950

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTKIIFMGTPQFAATVLKGLIDSNQYEILAVVTQPDRKVGRKQELRMTPVKELALTVN
CCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCHHHCCCHHHCCCCCHHHEEEEEC
LPVLQPEKLSGSVEMTQIMTLLESGEVGIVTAAFGQFLPGKLLDVAQFAVNTHASLLPKY
CCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
RGGAPIHYAIMNGEKEAGVTIMEMIRKMDAGDMIAQDSTPILEDDNVGTMFEKLALVGRD
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLLETLPKYLSGQLKAQAQNEDEVTFSPNISPEEEKIDWNKSAREIFNKVRGMNPFPVAH
HHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
TTWNGERFKIYETKVVDDSVGNLQAGEIVEKTKKSLKVATGEGLLELLFVQPAGKPKMDI
EECCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCHHHH
VSFLNGLGQKIQVGDKFGD
HHHHHHCCCEEECCCCCCC
>Mature Secondary Structure 
TKTKIIFMGTPQFAATVLKGLIDSNQYEILAVVTQPDRKVGRKQELRMTPVKELALTVN
CCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCHHHCCCHHHCCCCCHHHEEEEEC
LPVLQPEKLSGSVEMTQIMTLLESGEVGIVTAAFGQFLPGKLLDVAQFAVNTHASLLPKY
CCCCCCHHCCCCHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
RGGAPIHYAIMNGEKEAGVTIMEMIRKMDAGDMIAQDSTPILEDDNVGTMFEKLALVGRD
CCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
LLLETLPKYLSGQLKAQAQNEDEVTFSPNISPEEEKIDWNKSAREIFNKVRGMNPFPVAH
HHHHHHHHHHCCCCEECCCCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
TTWNGERFKIYETKVVDDSVGNLQAGEIVEKTKKSLKVATGEGLLELLFVQPAGKPKMDI
EECCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCHHHH
VSFLNGLGQKIQVGDKFGD
HHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA