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The map label for this gene is tuf

Identifier: 15673843

GI number: 15673843

Start: 1929364

End: 1930551

Strand: Reverse

Name: tuf

Synonym: L0371

Alternate gene names: 15673843

Gene position: 1930551-1929364 (Counterclockwise)

Preceding gene: 15673845

Following gene: 15673840

Centisome position: 81.61

GC content: 44.36

Gene sequence:

>1188_bases
ATGGCTAAAGAAGTATACGACCGTAGCAAACCACACGTTAATATTGGTACTATCGGACACGTTGACCATGGTAAAACTAC
CCTCTCAGCTGCAATCTCTAAAGTATTGTCTGACAAAGGTTACTCTAAAGCGACTGATTTCGCATCTATCGATGCTGCTC
CAGAAGAACGCGAACGTGGTATCACAATCAACACTGCTCACATCGAGTACGAAACTGAAAAACGTCACTATGCCCACATC
GACGCTCCAGGTCACGCGGACTACGTTAAAAATATGATCACTGGTGCTGCCCAAATGGACGGTGCAATCCTCGTTGTTGC
TGCAACTGATGGACCAATGCCACAAACTCGTGAACACATCTTGCTTTCACGTCAAGTTGGTGTTAAATACCTTATCGTCT
TCCTTAACAAGGCTGACCTTGTTGATGATGAAGAATTGATGGAACTCGTTGAAATGGAAGTTCGTGACCTCTTGAGCGAA
TACGACTTCCCAGGTGACGATATTCCTGTAATCGCTGGTTCAGCACTTGGTGCTTTGAACGGTGAACCACAATGGGTTGC
TAAAGTTGAAGAATTGATGGACATCGTTGATGAATACATCCCAACTCCAGAACGCGACACTGACAAACCACTCCTTCTTC
CAGTCGAAGACGTATTCTCTATCACTGGTCGTGGTACAGTTGCTTCAGGACGTATCGAACGTGGTACTGTTAAAGTTGGT
GACGAAGTTGAAATCGTTGGTATCAAAGAAGAAACTAAAAAAGCTGTTGTTACTGGTATCGAAATGTTCCGTAAAACACT
TACTGAAGGTCTTGCTGGTGATAACGTCGGTGCACTTCTCCGTGGTATTCAACGTGACGAAATCGAACGTGGTCAAGTTA
TTGCTAAACCAGGTTCAATCACTCCACACAAACTTTTCGAAGGTGAAGTTTACGTATTGAGCAAAGAAGAAGGCGGACGT
CACACTCCATTCTTCGACAACTACCGTCCTCAATTCTACTTCCACACAACTGACGTTACTGGTTCAGTTAAACTTCCAGA
AGGAACTGAAATGGTAATGCCTGGTGACAACGTGCATATCGACGTTGAATTGATCCACCCAGTTGCGATCGAACAAGGTA
CTACTTTCTCTATCCGTGAAGGTGGACGTACTGTTGGTTCAGGTATCGTTGCTGAAATCAAAGCTTAA

Upstream 100 bases:

>100_bases
GCATTTCAAAACTATTTTAAGTATAATGATAAAGAACGGTAGTTTGCTTTATGCAGACTACCTATAATAAAACAAATTCC
CCAAAGGAGACATTTTTAAA

Downstream 100 bases:

>100_bases
TTATAAATCTAAGCAAATTGGAGCTGTCATTTATGACAGTTCTTTTTTTTTTAATTTTCTTAAAATAAAAACCCCCAAAA
GGGGAGTTTTTTAAATTTCT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEVYDRSKPHVNIGTIGHVDHGKTTLSAAISKVLSDKGYSKATDFASIDAAPEERERGITINTAHIEYETEKRHYAHI
DAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLSRQVGVKYLIVFLNKADLVDDEELMELVEMEVRDLLSE
YDFPGDDIPVIAGSALGALNGEPQWVAKVEELMDIVDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIERGTVKVG
DEVEIVGIKEETKKAVVTGIEMFRKTLTEGLAGDNVGALLRGIQRDEIERGQVIAKPGSITPHKLFEGEVYVLSKEEGGR
HTPFFDNYRPQFYFHTTDVTGSVKLPEGTEMVMPGDNVHIDVELIHPVAIEQGTTFSIREGGRTVGSGIVAEIKA

Sequences:

>Translated_395_residues
MAKEVYDRSKPHVNIGTIGHVDHGKTTLSAAISKVLSDKGYSKATDFASIDAAPEERERGITINTAHIEYETEKRHYAHI
DAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLSRQVGVKYLIVFLNKADLVDDEELMELVEMEVRDLLSE
YDFPGDDIPVIAGSALGALNGEPQWVAKVEELMDIVDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIERGTVKVG
DEVEIVGIKEETKKAVVTGIEMFRKTLTEGLAGDNVGALLRGIQRDEIERGQVIAKPGSITPHKLFEGEVYVLSKEEGGR
HTPFFDNYRPQFYFHTTDVTGSVKLPEGTEMVMPGDNVHIDVELIHPVAIEQGTTFSIREGGRTVGSGIVAEIKA
>Mature_394_residues
AKEVYDRSKPHVNIGTIGHVDHGKTTLSAAISKVLSDKGYSKATDFASIDAAPEERERGITINTAHIEYETEKRHYAHID
APGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLSRQVGVKYLIVFLNKADLVDDEELMELVEMEVRDLLSEY
DFPGDDIPVIAGSALGALNGEPQWVAKVEELMDIVDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIERGTVKVGD
EVEIVGIKEETKKAVVTGIEMFRKTLTEGLAGDNVGALLRGIQRDEIERGQVIAKPGSITPHKLFEGEVYVLSKEEGGRH
TPFFDNYRPQFYFHTTDVTGSVKLPEGTEMVMPGDNVHIDVELIHPVAIEQGTTFSIREGGRTVGSGIVAEIKA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=55.8080808080808, Blast_Score=468, Evalue=1e-132,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=30.4740406320542, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=30.6997742663657, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.045871559633, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.045871559633, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI54607086, Length=312, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=30.6122448979592, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=30.6122448979592, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=30.6122448979592, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=31.2121212121212, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4503507, Length=280, Percent_Identity=30.3571428571429, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI157426893, Length=290, Percent_Identity=28.2758620689655, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=71.7557251908397, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=71.7557251908397, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=339, Percent_Identity=30.9734513274336, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=32.129963898917, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789108, Length=338, Percent_Identity=29.8816568047337, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788922, Length=332, Percent_Identity=27.4096385542169, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=49, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=44.559585492228, Blast_Score=333, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=443, Percent_Identity=30.0225733634311, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=443, Percent_Identity=30.0225733634311, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI32566629, Length=338, Percent_Identity=30.7692307692308, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=428, Percent_Identity=28.0373831775701, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=28.5388127853881, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=28.5388127853881, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17506081, Length=289, Percent_Identity=26.9896193771626, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=41.4965986394558, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17509919, Length=285, Percent_Identity=29.1228070175439, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.7056277056277, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=62.4365482233503, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.8558558558559, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.8558558558559, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6322937, Length=347, Percent_Identity=26.5129682997118, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=278, Percent_Identity=28.0575539568345, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=326, Percent_Identity=28.5276073619632, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=281, Percent_Identity=32.0284697508897, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=55.6410256410256, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=55.6410256410256, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=49.0956072351421, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=30.6487695749441, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=30.6487695749441, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=30.6487695749441, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=30.6487695749441, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=30.2013422818792, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=30.2013422818792, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.9078947368421, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550900, Length=454, Percent_Identity=29.0748898678414, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.2325581395349, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=29.6428571428571, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=29.6428571428571, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_LACLA (Q9CEI0)

Other databases:

- EMBL:   AE005176
- PIR:   E86857
- RefSeq:   NP_268018.1
- ProteinModelPortal:   Q9CEI0
- SMR:   Q9CEI0
- GeneID:   1115526
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0371
- NMPDR:   fig|272623.1.peg.1907
- HOGENOM:   HBG307581
- OMA:   AACIEEL
- ProtClustDB:   PRK12736
- BioCyc:   LLAC272623:L0371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43212; Mature: 43081

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEVYDRSKPHVNIGTIGHVDHGKTTLSAAISKVLSDKGYSKATDFASIDAAPEERERG
CCCCHHCCCCCEEEECEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
ITINTAHIEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
LLSRQVGVKYLIVFLNKADLVDDEELMELVEMEVRDLLSEYDFPGDDIPVIAGSALGALN
HHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCC
GEPQWVAKVEELMDIVDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIERGTVKVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCEECCEEEEC
DEVEIVGIKEETKKAVVTGIEMFRKTLTEGLAGDNVGALLRGIQRDEIERGQVIAKPGSI
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHCCCEEEECCCCC
TPHKLFEGEVYVLSKEEGGRHTPFFDNYRPQFYFHTTDVTGSVKLPEGTEMVMPGDNVHI
CHHEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEEE
DVELIHPVAIEQGTTFSIREGGRTVGSGIVAEIKA
EEEEECEEEECCCCEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure 
AKEVYDRSKPHVNIGTIGHVDHGKTTLSAAISKVLSDKGYSKATDFASIDAAPEERERG
CCCHHCCCCCEEEECEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
ITINTAHIEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHH
LLSRQVGVKYLIVFLNKADLVDDEELMELVEMEVRDLLSEYDFPGDDIPVIAGSALGALN
HHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCC
GEPQWVAKVEELMDIVDEYIPTPERDTDKPLLLPVEDVFSITGRGTVASGRIERGTVKVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCCEEECCCEECCEEEEC
DEVEIVGIKEETKKAVVTGIEMFRKTLTEGLAGDNVGALLRGIQRDEIERGQVIAKPGSI
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHCCCEEEECCCCC
TPHKLFEGEVYVLSKEEGGRHTPFFDNYRPQFYFHTTDVTGSVKLPEGTEMVMPGDNVHI
CHHEEECCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCEEE
DVELIHPVAIEQGTTFSIREGGRTVGSGIVAEIKA
EEEEECEEEECCCCEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471