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The map label for this gene is cysS

Identifier: 15673833

GI number: 15673833

Start: 1918254

End: 1919600

Strand: Reverse

Name: cysS

Synonym: L0348

Alternate gene names: 15673833

Gene position: 1919600-1918254 (Counterclockwise)

Preceding gene: 15673834

Following gene: 15673832

Centisome position: 81.15

GC content: 36.3

Gene sequence:

>1347_bases
ATGAAAATATATAATACTTATAGTCGACAGCTAGAAGATTTTCAGCCGATTGAACCTGGAAAAGTAAAAATGTATGTCTG
CGGACCGACCGTTTATAATTACATCCATGTGGGAAATGCTCGTTCTGTTGTTGCTTTTGATTTGGTTAGAAAATATTTGG
AATTTCGAGGATTTGAAGTTCAATATATCTCTAATTTCACAGATGTCGATGATAAAATTATCAACGGTGCAAAAGCTGCA
AACATGACTACGACAGATTTTTCTGAACGTTATATTGCTGCTTTTTATGAAGATACTGATGCTTTAAATGTTAAAAGAGC
CAGCCAAAATCCGAAAGCAACAGAATTCATCGAAGCGATGATTGAGTTTATTCAAGAACTAGTGGATAAAGAATTTGCTT
ATGTTTCTCAAGGAGACGTTTATTTCCGTGTATCTAAATCTAAAGATTACGCTAAATTAGCAAATAAAAATCTTGCAGAT
TTACTTGCAGGGGCTTCTGGACGTACGGATGAAGAAACAAAACTTAAAGAATCACCAGCCGATTTTGCTCTTTGGAAATC
CGCAAAAGCTGATGAAGTTTCTTGGCAAGCTCCTTGGGGAGCTGGCCGTCCAGGCTGGCATATTGAATGTTCAGTCATGT
CAACTTCACTATTAGGCGAAACAATTGACATTCATGGTGGAGGAGCAGATTTAGAATTTCCACACCATACAAATGAAATT
GCACAATCTGAAGCAAAAACTGGGCAAAAGTTTGTTAATTATTGGATGCACAATGGATTCGTAAATGTCGATGGTGAAAA
AATGTCTAAATCACTAGGCAATTTCACAACTGTTCATGAACTCCTTCAAGTAGTTAATCCACAAATTTTACGTTTCTTTT
TAGCAACTACTCATTATAGAAGACCAGTCAATTTTACTGATGATGCACTGACAGAAGCTGAAAATAATATCAAAAAAATT
GAAAATGCTTATCGTCATTTGGATGAGCAAGCTGAGTCTAATTTGTCAGCACTTACGACTTTCAGAAATGATTTTGTTGC
TGCCATGGATGAAGATTTCAATATTGCCAATGGAATGACTGTTTTTTATGACTTTGTTTCTTGGGTTAATAAAGGAAACG
GTGGGCCTGAGGTCAAAGAATTTTTTGACCAAGTTTTAGAAATTTTAGGGATTAAATTTAAATTTGAGCAAAGTTTGGAC
AGCGAAATTGAAGCCATGATTGAAGCAAGACAATTGGCACGAGAAGTCCGTGATTTTGCGAAGTCAGATGAAATTCGTGA
CGCTTTGAAAGCTCAAGGGATTGTTCTTGAAGATACAAAAGACGGGGTACGGTGGCATCGTGAATAA

Upstream 100 bases:

>100_bases
TATTGGATATCCTGCTTATACGGGGATTCCATTGCTCTATTTACCAATACGTTCTCTTATCACCTCTATTGTTTATGATG
AAGATTAAGAAGGAAATAGA

Downstream 100 bases:

>100_bases
GCAACAAGCAGAACTTTTGAATGGTATTGCATTGGCCTATATTGGGGATGCGATTTATGAGGTCTTTGTGCGTGAGTACT
TATTAGATAAAGGCCTGACA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKIYNTYSRQLEDFQPIEPGKVKMYVCGPTVYNYIHVGNARSVVAFDLVRKYLEFRGFEVQYISNFTDVDDKIINGAKAA
NMTTTDFSERYIAAFYEDTDALNVKRASQNPKATEFIEAMIEFIQELVDKEFAYVSQGDVYFRVSKSKDYAKLANKNLAD
LLAGASGRTDEETKLKESPADFALWKSAKADEVSWQAPWGAGRPGWHIECSVMSTSLLGETIDIHGGGADLEFPHHTNEI
AQSEAKTGQKFVNYWMHNGFVNVDGEKMSKSLGNFTTVHELLQVVNPQILRFFLATTHYRRPVNFTDDALTEAENNIKKI
ENAYRHLDEQAESNLSALTTFRNDFVAAMDEDFNIANGMTVFYDFVSWVNKGNGGPEVKEFFDQVLEILGIKFKFEQSLD
SEIEAMIEARQLAREVRDFAKSDEIRDALKAQGIVLEDTKDGVRWHRE

Sequences:

>Translated_448_residues
MKIYNTYSRQLEDFQPIEPGKVKMYVCGPTVYNYIHVGNARSVVAFDLVRKYLEFRGFEVQYISNFTDVDDKIINGAKAA
NMTTTDFSERYIAAFYEDTDALNVKRASQNPKATEFIEAMIEFIQELVDKEFAYVSQGDVYFRVSKSKDYAKLANKNLAD
LLAGASGRTDEETKLKESPADFALWKSAKADEVSWQAPWGAGRPGWHIECSVMSTSLLGETIDIHGGGADLEFPHHTNEI
AQSEAKTGQKFVNYWMHNGFVNVDGEKMSKSLGNFTTVHELLQVVNPQILRFFLATTHYRRPVNFTDDALTEAENNIKKI
ENAYRHLDEQAESNLSALTTFRNDFVAAMDEDFNIANGMTVFYDFVSWVNKGNGGPEVKEFFDQVLEILGIKFKFEQSLD
SEIEAMIEARQLAREVRDFAKSDEIRDALKAQGIVLEDTKDGVRWHRE
>Mature_448_residues
MKIYNTYSRQLEDFQPIEPGKVKMYVCGPTVYNYIHVGNARSVVAFDLVRKYLEFRGFEVQYISNFTDVDDKIINGAKAA
NMTTTDFSERYIAAFYEDTDALNVKRASQNPKATEFIEAMIEFIQELVDKEFAYVSQGDVYFRVSKSKDYAKLANKNLAD
LLAGASGRTDEETKLKESPADFALWKSAKADEVSWQAPWGAGRPGWHIECSVMSTSLLGETIDIHGGGADLEFPHHTNEI
AQSEAKTGQKFVNYWMHNGFVNVDGEKMSKSLGNFTTVHELLQVVNPQILRFFLATTHYRRPVNFTDDALTEAENNIKKI
ENAYRHLDEQAESNLSALTTFRNDFVAAMDEDFNIANGMTVFYDFVSWVNKGNGGPEVKEFFDQVLEILGIKFKFEQSLD
SEIEAMIEARQLAREVRDFAKSDEIRDALKAQGIVLEDTKDGVRWHRE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=504, Percent_Identity=30.952380952381, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI303304987, Length=230, Percent_Identity=40.4347826086956, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI62240992, Length=230, Percent_Identity=40.4347826086956, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI10835051, Length=230, Percent_Identity=40.4347826086956, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI21269877, Length=230, Percent_Identity=40.4347826086956, Blast_Score=185, Evalue=7e-47,
Organism=Escherichia coli, GI1786737, Length=465, Percent_Identity=41.9354838709677, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17509795, Length=246, Percent_Identity=36.9918699186992, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17509797, Length=246, Percent_Identity=36.9918699186992, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324082, Length=252, Percent_Identity=41.2698412698413, Blast_Score=198, Evalue=1e-51,
Organism=Drosophila melanogaster, GI19922218, Length=506, Percent_Identity=33.9920948616601, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI19922364, Length=230, Percent_Identity=38.695652173913, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_LACLA (Q9CEJ0)

Other databases:

- EMBL:   AE005176
- PIR:   C86856
- RefSeq:   NP_268008.1
- ProteinModelPortal:   Q9CEJ0
- SMR:   Q9CEJ0
- GeneID:   1115516
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0348
- NMPDR:   fig|272623.1.peg.1897
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   LLAC272623:L0348-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50946; Mature: 50946

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYNTYSRQLEDFQPIEPGKVKMYVCGPTVYNYIHVGNARSVVAFDLVRKYLEFRGFEV
CCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHHCCCEE
QYISNFTDVDDKIINGAKAANMTTTDFSERYIAAFYEDTDALNVKRASQNPKATEFIEAM
EEECCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
IEFIQELVDKEFAYVSQGDVYFRVSKSKDYAKLANKNLADLLAGASGRTDEETKLKESPA
HHHHHHHHHHHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHCCCC
DFALWKSAKADEVSWQAPWGAGRPGWHIECSVMSTSLLGETIDIHGGGADLEFPHHTNEI
CHHHHCCCCCCCCCEECCCCCCCCCCEEEEEEHHHHHCCCEEEECCCCCCCCCCCCHHHH
AQSEAKTGQKFVNYWMHNGFVNVDGEKMSKSLGNFTTVHELLQVVNPQILRFFLATTHYR
HHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCC
RPVNFTDDALTEAENNIKKIENAYRHLDEQAESNLSALTTFRNDFVAAMDEDFNIANGMT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHH
VFYDFVSWVNKGNGGPEVKEFFDQVLEILGIKFKFEQSLDSEIEAMIEARQLAREVRDFA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
KSDEIRDALKAQGIVLEDTKDGVRWHRE
HHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKIYNTYSRQLEDFQPIEPGKVKMYVCGPTVYNYIHVGNARSVVAFDLVRKYLEFRGFEV
CCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHHCCCEE
QYISNFTDVDDKIINGAKAANMTTTDFSERYIAAFYEDTDALNVKRASQNPKATEFIEAM
EEECCCCCHHHHHHCCCHHCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHH
IEFIQELVDKEFAYVSQGDVYFRVSKSKDYAKLANKNLADLLAGASGRTDEETKLKESPA
HHHHHHHHHHHHHEEECCCEEEEEECCCHHHHHHCCCHHHHHCCCCCCCCCHHHHHCCCC
DFALWKSAKADEVSWQAPWGAGRPGWHIECSVMSTSLLGETIDIHGGGADLEFPHHTNEI
CHHHHCCCCCCCCCEECCCCCCCCCCEEEEEEHHHHHCCCEEEECCCCCCCCCCCCHHHH
AQSEAKTGQKFVNYWMHNGFVNVDGEKMSKSLGNFTTVHELLQVVNPQILRFFLATTHYR
HHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHCC
RPVNFTDDALTEAENNIKKIENAYRHLDEQAESNLSALTTFRNDFVAAMDEDFNIANGMT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHH
VFYDFVSWVNKGNGGPEVKEFFDQVLEILGIKFKFEQSLDSEIEAMIEARQLAREVRDFA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
KSDEIRDALKAQGIVLEDTKDGVRWHRE
HHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471