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The map label for this gene is asnC

Identifier: 15673810

GI number: 15673810

Start: 1895088

End: 1896431

Strand: Reverse

Name: asnC

Synonym: L0345

Alternate gene names: 15673810

Gene position: 1896431-1895088 (Counterclockwise)

Preceding gene: 15673811

Following gene: 15673808

Centisome position: 80.17

GC content: 37.87

Gene sequence:

>1344_bases
ATGAAAGACTTAATTTCAATCATCGACGTCAAAGACCACGTTGGTGAAACAGTAAAAATCGGCGCTTGGGTTGCTGACAA
ATCAGGTAAAGGGAAACTTCAATTTCTTCAACTTCGCGATGGAACAGCATTTTTCCAAGCTGTTGTTTTCAAACCAAATA
TGATTGAAAACTTTGGTGAAGAAGAAGGAACAGCTAAATTTGATGAAATTAAACATCTCTCACAAGAAACTTCAGTTTAT
GTGACAGGTGTCGTTAAAGAAGATTCACGTTCAAAATTTGGTTATGAACTTGATGTGACTGATTTGGAAGTGATTGGTCA
ATCACATGATTATCCAATTACGCCAAAAGAACACGGCGTTGAATTCCTCCTTGATAACCGCCATTTATGGTTGCGTTCAA
AACGTCAAATGGCAATGATGCAAGTGCGTAATGCAATCATCTATGCAAGTTATGATTTCTTTGCGAAAAATGGCTTCATT
AAGTTTGATAGTCCAATTCTTTCAGGAAATGCTGCTGAAAACACAACAGAACTCTTTGAAACAGATTATTTTGGTAATTC
TGCTTTCTTGTCACAATCAGGACAACTTTATCTTGAAGCTGGAGCAATGGCTCTTGGACGTGTTTTCGACTTTGGACCAG
TATTCCGTGCTGAAAAATCAAAAACTCGTCGTCATTTGACAGAATTTTGGATGATGGATGCAGAATATCCATTTGTTACT
CATGATGAATCATTGGATATTCAAGAAGCGTATGTGAAAGCTTTGATTCAAGGTGTTTTGGATAATGCTGCTTATGCTCT
TGAAACACTTGAACGTGATACAAGCATGTTGCAAAAGTATATTGATACACCTTTCAAACGTGTTTCCTATGATGCTGCTA
TTGATTTGTTGCAAGCACACGAAAATGATGAAGATACAGATTATGAGCATGTAGAACATGGGGATGACTTCGGTTCGCCT
CATGAAACATGGATTTCAAATTACTATGGTGTTCCAACATTTATCGTCAACTATCCTGCAAGTTTTAAAGCTTTCTACAT
GAAACCAGTCCCTGGTAACCCAGAACGCGTTTTGTGTGCAGACCTTTTGGCTCCAGAAGGCTATGGCGAAATCATTGGTG
GTTCAGAACGTGAAACTGATTATGATTTGTTACTTAAGAAAATTGCTGATTTCGGTTTAGATCCTAAAGATTATGATTGG
TATTTAGAACTTCGTAAATTTGGTTCAGTTCCTCATGCTGGTTTCGGACTTGGTCTTGAACGTATGGTTACTTTTGTCGC
TGGGACAGAACATATTCGTGAAGCAATTCCATTCCCACGTATGATTAACCGTATCCAACCTTAA

Upstream 100 bases:

>100_bases
AATTCAAAGAAGATTTAAAAACTTTAAAAGAAATCTGGCTTGAAAAGTTATAAAAGTATTATTTAAAAATTATAAAATTA
AAATATAGAAGGACTTATTT

Downstream 100 bases:

>100_bases
TAAAAATAAAAAACCTCTACTTTAAGAGGTTTTTTTATTATTTTGAAGAGGAAGCGGAAGAAGCTTGGGCTGCAGTAACA
TCTTCTTTAGTGACTAAGAT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKDLISIIDVKDHVGETVKIGAWVADKSGKGKLQFLQLRDGTAFFQAVVFKPNMIENFGEEEGTAKFDEIKHLSQETSVY
VTGVVKEDSRSKFGYELDVTDLEVIGQSHDYPITPKEHGVEFLLDNRHLWLRSKRQMAMMQVRNAIIYASYDFFAKNGFI
KFDSPILSGNAAENTTELFETDYFGNSAFLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYPFVT
HDESLDIQEAYVKALIQGVLDNAAYALETLERDTSMLQKYIDTPFKRVSYDAAIDLLQAHENDEDTDYEHVEHGDDFGSP
HETWISNYYGVPTFIVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSERETDYDLLLKKIADFGLDPKDYDW
YLELRKFGSVPHAGFGLGLERMVTFVAGTEHIREAIPFPRMINRIQP

Sequences:

>Translated_447_residues
MKDLISIIDVKDHVGETVKIGAWVADKSGKGKLQFLQLRDGTAFFQAVVFKPNMIENFGEEEGTAKFDEIKHLSQETSVY
VTGVVKEDSRSKFGYELDVTDLEVIGQSHDYPITPKEHGVEFLLDNRHLWLRSKRQMAMMQVRNAIIYASYDFFAKNGFI
KFDSPILSGNAAENTTELFETDYFGNSAFLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYPFVT
HDESLDIQEAYVKALIQGVLDNAAYALETLERDTSMLQKYIDTPFKRVSYDAAIDLLQAHENDEDTDYEHVEHGDDFGSP
HETWISNYYGVPTFIVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSERETDYDLLLKKIADFGLDPKDYDW
YLELRKFGSVPHAGFGLGLERMVTFVAGTEHIREAIPFPRMINRIQP
>Mature_447_residues
MKDLISIIDVKDHVGETVKIGAWVADKSGKGKLQFLQLRDGTAFFQAVVFKPNMIENFGEEEGTAKFDEIKHLSQETSVY
VTGVVKEDSRSKFGYELDVTDLEVIGQSHDYPITPKEHGVEFLLDNRHLWLRSKRQMAMMQVRNAIIYASYDFFAKNGFI
KFDSPILSGNAAENTTELFETDYFGNSAFLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYPFVT
HDESLDIQEAYVKALIQGVLDNAAYALETLERDTSMLQKYIDTPFKRVSYDAAIDLLQAHENDEDTDYEHVEHGDDFGSP
HETWISNYYGVPTFIVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSERETDYDLLLKKIADFGLDPKDYDW
YLELRKFGSVPHAGFGLGLERMVTFVAGTEHIREAIPFPRMINRIQP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=456, Percent_Identity=32.8947368421053, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI4758762, Length=453, Percent_Identity=33.3333333333333, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI45439306, Length=471, Percent_Identity=28.0254777070064, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1787161, Length=474, Percent_Identity=33.7552742616034, Blast_Score=257, Evalue=9e-70,
Organism=Caenorhabditis elegans, GI71984122, Length=451, Percent_Identity=37.2505543237251, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17556372, Length=379, Percent_Identity=30.0791556728232, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17551876, Length=337, Percent_Identity=30.5637982195846, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321807, Length=451, Percent_Identity=31.9290465631929, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319872, Length=463, Percent_Identity=31.317494600432, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6323011, Length=450, Percent_Identity=28, Blast_Score=139, Evalue=9e-34,
Organism=Drosophila melanogaster, GI19921528, Length=438, Percent_Identity=34.4748858447489, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI19922416, Length=471, Percent_Identity=31.6348195329087, Blast_Score=221, Evalue=7e-58,
Organism=Drosophila melanogaster, GI17136276, Length=475, Percent_Identity=27.1578947368421, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_LACLA (Q9CEK9)

Other databases:

- EMBL:   AE005176
- PIR:   D86853
- RefSeq:   NP_267985.1
- ProteinModelPortal:   Q9CEK9
- SMR:   Q9CEK9
- GeneID:   1115493
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0345
- NMPDR:   fig|272623.1.peg.1874
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   LLAC272623:L0345-MONOMER
- BRENDA:   6.1.1.22
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 50841; Mature: 50841

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLISIIDVKDHVGETVKIGAWVADKSGKGKLQFLQLRDGTAFFQAVVFKPNMIENFGE
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHCCC
EEGTAKFDEIKHLSQETSVYVTGVVKEDSRSKFGYELDVTDLEVIGQSHDYPITPKEHGV
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEECCCHHHHHCCCCCCCCCCHHCCC
EFLLDNRHLWLRSKRQMAMMQVRNAIIYASYDFFAKNGFIKFDSPILSGNAAENTTELFE
EEEECCCEEEHHHHHHHHHHHHHHEEEEEEEHHHHCCCEEEECCCEECCCCCCCHHHHHH
TDYFGNSAFLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYPFVT
HCCCCCCCEECCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
HDESLDIQEAYVKALIQGVLDNAAYALETLERDTSMLQKYIDTPFKRVSYDAAIDLLQAH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
ENDEDTDYEHVEHGDDFGSPHETWISNYYGVPTFIVNYPASFKAFYMKPVPGNPERVLCA
CCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEH
DLLAPEGYGEIIGGSERETDYDLLLKKIADFGLDPKDYDWYLELRKFGSVPHAGFGLGLE
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHH
RMVTFVAGTEHIREAIPFPRMINRIQP
HHHHHHHCHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MKDLISIIDVKDHVGETVKIGAWVADKSGKGKLQFLQLRDGTAFFQAVVFKPNMIENFGE
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHCCC
EEGTAKFDEIKHLSQETSVYVTGVVKEDSRSKFGYELDVTDLEVIGQSHDYPITPKEHGV
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCEEECCCHHHHHCCCCCCCCCCHHCCC
EFLLDNRHLWLRSKRQMAMMQVRNAIIYASYDFFAKNGFIKFDSPILSGNAAENTTELFE
EEEECCCEEEHHHHHHHHHHHHHHEEEEEEEHHHHCCCEEEECCCEECCCCCCCHHHHHH
TDYFGNSAFLSQSGQLYLEAGAMALGRVFDFGPVFRAEKSKTRRHLTEFWMMDAEYPFVT
HCCCCCCCEECCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE
HDESLDIQEAYVKALIQGVLDNAAYALETLERDTSMLQKYIDTPFKRVSYDAAIDLLQAH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
ENDEDTDYEHVEHGDDFGSPHETWISNYYGVPTFIVNYPASFKAFYMKPVPGNPERVLCA
CCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEH
DLLAPEGYGEIIGGSERETDYDLLLKKIADFGLDPKDYDWYLELRKFGSVPHAGFGLGLE
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHH
RMVTFVAGTEHIREAIPFPRMINRIQP
HHHHHHHCHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471