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Identifier: 15673759

GI number: 15673759

Start: 1836391

End: 1836954

Strand: Reverse

Name: bar

Synonym: L19745

Alternate gene names: 15673759

Gene position: 1836954-1836391 (Counterclockwise)

Preceding gene: 15673760

Following gene: 15673758

Centisome position: 77.65

GC content: 35.11

Gene sequence:

>564_bases
ATGGAGTTTAGATTAGCGAAAAAGTCTGATGCTAAAAGACTTTTAGAAATTTATAAACCTTATGTTGAAAAAACTGCCAT
TACTTTTGAGTATGAAGTCCCAACGATTGCAGAATTTGAAAAGCGAATTGAAAAAATAGGAAGTCGCTATCCTTATATTG
TGGCGATTGAAAATGACAAAATCATTGGTTATGCTTATGCTGGCGCTTATCGAGAACGGGCGGCTTATGATTGGGTGGTT
GAATTATCAATTTATTTAGATGAAAATGAACGGCAACATGGCGCTGGTAGTGCCCTTTACCAAAAGTTACTGACAGCTTT
ATCGGTACTTAATTATCAGCGTGCTTATGCTTGTATTACCTATCCAAATCCAGCAAGTGTTGCATTTCATAAAAAATTTG
GTTTTGAACAAATTGGACTTTTTCCAAAAGCGGGTTATAAGTTTGAACAATGGTATGGTATTGTTTGGTTAGAACGCTCT
CTCCAAACTACTGATAAAGTTACTGCGATAAAATTACTGACAGATTTGTCAGAGAATGAACTAGAAAAAATCTTGGCTAA
TTAG

Upstream 100 bases:

>100_bases
ATTTATCTCTGATAAAAAAGTGTTAAATAAAAATGTGTAGAAATTTCTGTCAGTAATTAATAAATGGTGTATAATAGTTA
AAAATGTAAAGGAGAAGGAA

Downstream 100 bases:

>100_bases
TCAAGATTTTTTATTATTTTATTGAAAAAGTAAAACATTTTGTGTATGGTAAGAAAAATATTAAAATAAAATTAAATAAA
TTAAAAGTAGAAAGGAAGAA

Product: acyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MEFRLAKKSDAKRLLEIYKPYVEKTAITFEYEVPTIAEFEKRIEKIGSRYPYIVAIENDKIIGYAYAGAYRERAAYDWVV
ELSIYLDENERQHGAGSALYQKLLTALSVLNYQRAYACITYPNPASVAFHKKFGFEQIGLFPKAGYKFEQWYGIVWLERS
LQTTDKVTAIKLLTDLSENELEKILAN

Sequences:

>Translated_187_residues
MEFRLAKKSDAKRLLEIYKPYVEKTAITFEYEVPTIAEFEKRIEKIGSRYPYIVAIENDKIIGYAYAGAYRERAAYDWVV
ELSIYLDENERQHGAGSALYQKLLTALSVLNYQRAYACITYPNPASVAFHKKFGFEQIGLFPKAGYKFEQWYGIVWLERS
LQTTDKVTAIKLLTDLSENELEKILAN
>Mature_187_residues
MEFRLAKKSDAKRLLEIYKPYVEKTAITFEYEVPTIAEFEKRIEKIGSRYPYIVAIENDKIIGYAYAGAYRERAAYDWVV
ELSIYLDENERQHGAGSALYQKLLTALSVLNYQRAYACITYPNPASVAFHKKFGFEQIGLFPKAGYKFEQWYGIVWLERS
LQTTDKVTAIKLLTDLSENELEKILAN

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=174, Percent_Identity=28.735632183908, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 21678; Mature: 21678

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRLAKKSDAKRLLEIYKPYVEKTAITFEYEVPTIAEFEKRIEKIGSRYPYIVAIENDK
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCE
IIGYAYAGAYRERAAYDWVVELSIYLDENERQHGAGSALYQKLLTALSVLNYQRAYACIT
EEEEEECCHHHHHHHEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
YPNPASVAFHKKFGFEQIGLFPKAGYKFEQWYGIVWLERSLQTTDKVTAIKLLTDLSENE
CCCCCCEEEEHHCCHHHHCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHH
LEKILAN
HHHHHCC
>Mature Secondary Structure
MEFRLAKKSDAKRLLEIYKPYVEKTAITFEYEVPTIAEFEKRIEKIGSRYPYIVAIENDK
CCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCE
IIGYAYAGAYRERAAYDWVVELSIYLDENERQHGAGSALYQKLLTALSVLNYQRAYACIT
EEEEEECCHHHHHHHEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
YPNPASVAFHKKFGFEQIGLFPKAGYKFEQWYGIVWLERSLQTTDKVTAIKLLTDLSENE
CCCCCCEEEEHHCCHHHHCCCCCCCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHCCHHH
LEKILAN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3240868; 8593056 [H]