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The map label for this gene is smpB

Identifier: 15673704

GI number: 15673704

Start: 1777208

End: 1777675

Strand: Reverse

Name: smpB

Synonym: L160560

Alternate gene names: 15673704

Gene position: 1777675-1777208 (Counterclockwise)

Preceding gene: 15673705

Following gene: 15673703

Centisome position: 75.15

GC content: 34.4

Gene sequence:

>468_bases
ATGGTAAAAAATACAGAAGATAAGCCTTTAGCTCAGAACAAAAAGGCTCGGCATGATTATGAAATTTACGAAACTTTTGA
AGCAGGAATTGTGCTGACAGGAACGGAGATTAAGTCTGTTCGTCAGGCAAAAATTCAATTAAAAGATGGATTTGCCAGAG
TTCGTAATGGAGAAGTTTGGTTATCAAATGTTCATATTGCTCCTTTTGAGCAGGGCAATATTTTTAATGTTGAAGAATTG
CGTACGCGGAAACTATTACTAAATAAAAAAGAAATTGCTAAGATTGATAAGGAATTATCAGGGACAGGAATTACTTTTAT
TCCTTTAAAAGTTTATTTGAAAAATGGCTTTGCTAAGGTTTTGATGGGGCTTGCCCGTGGTAAGAAAGATTACGATAAAC
GTGAAACGCTCAAACGTAAAGAACAAAATCGGGATATTGCAAGACAAATTAAAGCTTATAACCGTTAA

Upstream 100 bases:

>100_bases
TTACATTTTACATCAAGGGCTTATGAGTTGTTGGTCAGAGAGATTTTATCTGTCATTAAAAATCCTTAAATAAATGTTCA
TTTTGTGGTAAAATGAGGGC

Downstream 100 bases:

>100_bases
AGAAATCACTGATAAATCTGTCAGTGATTTTTTTACTGACAGAATTGTCAGCTTGCTGATAATTCTTTTATTTACAGCGA
AAATATGCCTTCTTTGTCAT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MVKNTEDKPLAQNKKARHDYEIYETFEAGIVLTGTEIKSVRQAKIQLKDGFARVRNGEVWLSNVHIAPFEQGNIFNVEEL
RTRKLLLNKKEIAKIDKELSGTGITFIPLKVYLKNGFAKVLMGLARGKKDYDKRETLKRKEQNRDIARQIKAYNR

Sequences:

>Translated_155_residues
MVKNTEDKPLAQNKKARHDYEIYETFEAGIVLTGTEIKSVRQAKIQLKDGFARVRNGEVWLSNVHIAPFEQGNIFNVEEL
RTRKLLLNKKEIAKIDKELSGTGITFIPLKVYLKNGFAKVLMGLARGKKDYDKRETLKRKEQNRDIARQIKAYNR
>Mature_155_residues
MVKNTEDKPLAQNKKARHDYEIYETFEAGIVLTGTEIKSVRQAKIQLKDGFARVRNGEVWLSNVHIAPFEQGNIFNVEEL
RTRKLLLNKKEIAKIDKELSGTGITFIPLKVYLKNGFAKVLMGLARGKKDYDKRETLKRKEQNRDIARQIKAYNR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=40, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_LACLA (Q9CEW2)

Other databases:

- EMBL:   AE005176
- PIR:   B86840
- RefSeq:   NP_267878.1
- ProteinModelPortal:   Q9CEW2
- SMR:   Q9CEW2
- GeneID:   1115385
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L160560
- NMPDR:   fig|272623.1.peg.1766
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   LLAC272623:L160560-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17948; Mature: 17948

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNTEDKPLAQNKKARHDYEIYETFEAGIVLTGTEIKSVRQAKIQLKDGFARVRNGEVW
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEE
LSNVHIAPFEQGNIFNVEELRTRKLLLNKKEIAKIDKELSGTGITFIPLKVYLKNGFAKV
EEEEEECCCCCCCEECHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHH
LMGLARGKKDYDKRETLKRKEQNRDIARQIKAYNR
HHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure
MVKNTEDKPLAQNKKARHDYEIYETFEAGIVLTGTEIKSVRQAKIQLKDGFARVRNGEVW
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEE
LSNVHIAPFEQGNIFNVEELRTRKLLLNKKEIAKIDKELSGTGITFIPLKVYLKNGFAKV
EEEEEECCCCCCCEECHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHH
LMGLARGKKDYDKRETLKRKEQNRDIARQIKAYNR
HHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471