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The map label for this gene is pstE

Identifier: 15673702

GI number: 15673702

Start: 1774836

End: 1775687

Strand: Reverse

Name: pstE

Synonym: L158188

Alternate gene names: 15673702

Gene position: 1775687-1774836 (Counterclockwise)

Preceding gene: 15673703

Following gene: 15673701

Centisome position: 75.06

GC content: 37.68

Gene sequence:

>852_bases
ATGAAGAAGAAAATTTTTATTGCTTTGATGGCCAGTGTGAGTTTATTTGCATTGGCAGCTTGTGGTTCTGGAAATAAACA
GGTCACAGCAGGTGGCTCAACTGCCTTGCAACCCATGGTTGAACATGCTTCTATGGCATATATGAAGCAAAATCCTGATG
AAATTATTATGGTTCAAGGTGGTGGTTCTGGTGTCGGATTGGCTCAAGTTGCGGCTGGTTCTTTCCAGATTGGGAATTCT
GATATTTTCGCTGAAGAAAAATCAGGAATAGCAGTTGATAAAATTATTGACCATAAAGTTGCTGTCATTGGTTTTGCACC
AATTATCAACAAAAAGTTAAAAGTTTCAAATTTGACTCAACAAGAACTGATTGATATTTTCACTGGTAAAATTACCAACT
GGAAAGAAGTAGGAGGTCAAGACCAAAAAATTACGGTCATTGGTCGTACTGCTGGTTCAGGAACGCGAGTAAATTTTGAT
AAATATGCTTTGAATGGTGCCACAGAAGTGAATGGTCCAACTCAAGATGCTTCAGGTTCTGTTGTCCAATTAGTTGGGCA
AACGCCAGGGGCTATTTCATATGTTGCTTTCAGTTACACCAACAAACCCGGTGTAAAGGCGGTTAATGTTGATGGTGTTG
AGCCAACTTATGACAATGTAAAAACTAATGCATGGAAAATTTGGTCATATGAGCATATGTACACCAACAAATCTCGTAAG
AACACAATCGAAAATAAATTTATCGAATACGTTCAAAATGATAAGAAAACCCTAGAGCAGCTTGGTTATATCCGTGTTTC
TGAAATGAAGGTTGACCGTGATGCTAAAGGAAATGTTGAAAAAATTAAATAA

Upstream 100 bases:

>100_bases
TTTTCAGAGTTGGGCATTTTCCCGTATTATTTACAAAACATTTACATAAGAACGCTATCATCAACCGCTCATTGATAGGC
CATTATGGTATAATTAAGCT

Downstream 100 bases:

>100_bases
TTACTGATGGAAATTAAACTTATCCTCCGTCAGTAAAAAAATTAGTCAAAATAAGTTTAATCAATAACTTCATTTGCATT
AATCAGATAGATGCAAATTA

Product: phosphate ABC transporter substrate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 1 [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKKIFIALMASVSLFALAACGSGNKQVTAGGSTALQPMVEHASMAYMKQNPDEIIMVQGGGSGVGLAQVAAGSFQIGNS
DIFAEEKSGIAVDKIIDHKVAVIGFAPIINKKLKVSNLTQQELIDIFTGKITNWKEVGGQDQKITVIGRTAGSGTRVNFD
KYALNGATEVNGPTQDASGSVVQLVGQTPGAISYVAFSYTNKPGVKAVNVDGVEPTYDNVKTNAWKIWSYEHMYTNKSRK
NTIENKFIEYVQNDKKTLEQLGYIRVSEMKVDRDAKGNVEKIK

Sequences:

>Translated_283_residues
MKKKIFIALMASVSLFALAACGSGNKQVTAGGSTALQPMVEHASMAYMKQNPDEIIMVQGGGSGVGLAQVAAGSFQIGNS
DIFAEEKSGIAVDKIIDHKVAVIGFAPIINKKLKVSNLTQQELIDIFTGKITNWKEVGGQDQKITVIGRTAGSGTRVNFD
KYALNGATEVNGPTQDASGSVVQLVGQTPGAISYVAFSYTNKPGVKAVNVDGVEPTYDNVKTNAWKIWSYEHMYTNKSRK
NTIENKFIEYVQNDKKTLEQLGYIRVSEMKVDRDAKGNVEKIK
>Mature_283_residues
MKKKIFIALMASVSLFALAACGSGNKQVTAGGSTALQPMVEHASMAYMKQNPDEIIMVQGGGSGVGLAQVAAGSFQIGNS
DIFAEEKSGIAVDKIIDHKVAVIGFAPIINKKLKVSNLTQQELIDIFTGKITNWKEVGGQDQKITVIGRTAGSGTRVNFD
KYALNGATEVNGPTQDASGSVVQLVGQTPGAISYVAFSYTNKPGVKAVNVDGVEPTYDNVKTNAWKIWSYEHMYTNKSRK
NTIENKFIEYVQNDKKTLEQLGYIRVSEMKVDRDAKGNVEKIK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 30561; Mature: 30561

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIFIALMASVSLFALAACGSGNKQVTAGGSTALQPMVEHASMAYMKQNPDEIIMVQG
CCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GGSGVGLAQVAAGSFQIGNSDIFAEEKSGIAVDKIIDHKVAVIGFAPIINKKLKVSNLTQ
CCCCCCHHHHCCCCEEECCCCEEEECCCCEEHHHHHCCEEEEEEEHHHHCCEEEECCCCH
QELIDIFTGKITNWKEVGGQDQKITVIGRTAGSGTRVNFDKYALNGATEVNGPTQDASGS
HHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCEEECHHEEECCCCCCCCCCCCCCCC
VVQLVGQTPGAISYVAFSYTNKPGVKAVNVDGVEPTYDNVKTNAWKIWSYEHMYTNKSRK
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCHHH
NTIENKFIEYVQNDKKTLEQLGYIRVSEMKVDRDAKGNVEKIK
HHHHHHHHHHHHCHHHHHHHHCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKKIFIALMASVSLFALAACGSGNKQVTAGGSTALQPMVEHASMAYMKQNPDEIIMVQG
CCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GGSGVGLAQVAAGSFQIGNSDIFAEEKSGIAVDKIIDHKVAVIGFAPIINKKLKVSNLTQ
CCCCCCHHHHCCCCEEECCCCEEEECCCCEEHHHHHCCEEEEEEEHHHHCCEEEECCCCH
QELIDIFTGKITNWKEVGGQDQKITVIGRTAGSGTRVNFDKYALNGATEVNGPTQDASGS
HHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCCCEEECHHEEECCCCCCCCCCCCCCCC
VVQLVGQTPGAISYVAFSYTNKPGVKAVNVDGVEPTYDNVKTNAWKIWSYEHMYTNKSRK
EEEEECCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHHCCCHHH
NTIENKFIEYVQNDKKTLEQLGYIRVSEMKVDRDAKGNVEKIK
HHHHHHHHHHHHCHHHHHHHHCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12354221 [H]