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The map label for this gene is murD

Identifier: 15673572

GI number: 15673572

Start: 1633545

End: 1634897

Strand: Reverse

Name: murD

Synonym: L0237

Alternate gene names: 15673572

Gene position: 1634897-1633545 (Counterclockwise)

Preceding gene: 15673573

Following gene: 15673571

Centisome position: 69.11

GC content: 35.62

Gene sequence:

>1353_bases
ATGAAAAAAATAACGAAATTTAAAGATCAAAAAATCTTAGTTCTTGGACTTGCCCGTTCTGGAATGGCTGCTGCCCTTGT
GCTCAATGAGTTGGGTGCGATTGTTACGGTCAATGATGGCAAACCTTTTGAAGAAAATAAAGAAGCGCAATTACTTTTAG
AAGAAGGAATTAAAGTCATCACAGGGAGTCATCCTATTGATTTATTGGATGAAGATTTTGCACTAATGGTTAAAAATCCA
GGAATTCGTTATGATAACCCAATGGTTGAACGAGCTGAAGCATTGAATATTCCTGTAATTACTGAGGTTGAACTTGCTTA
TCTCATCAGTGAAGCTCCAATCATTGGAATTACCGGAACCAATGGGAAAACAACGACGACAACATTGATTGCTGATATTT
TAAATGCCGATGGTCAGTCAGCAAAATTGTCAGGGAATATTGGTTTTCCAGCGTCAGAAGTAGCCGAAAAAGCGAGTGCT
TCTGATACATTAGTGATGGAACTTTCAAGTTTTCAATTGATGGGCATTGATAGCTTCCGCCCTAAAATTGCCTTAATTAC
AAATTTATTTTCAGCTCATTTAGACTATCATGGTTCGCAAAAAGCCTATGAAGCTGCAAAATGGCGAATTCAAGAAAATA
TGACCTCAGATGATTTCTTGATTCTCAATTTTAATCAAGAAAAATGTCGTAATTTGGCTGACAAAACGAAAGCGACTGTT
CTTGCTTTTTCAACAAAAGAAAAAGTTAATGGTGCTTATTCTAAAGATGGAAAAATTTATTTTAATGATGAATATATCAT
GGAAGTTTCTGAACTTTCACTTCCTGGTGAGCATAATCTAGAAAATGCTTTAGCAGCCATTGTAGCTTCAAAATTACAAG
GAACAAAAAATGAAGCGATTGTAGAAGTACTGACAAGTTTTGCTGGTGTAAAACATCGTCTGCAATATCTTGGCGAAATT
GATGGTCGTAAAGTCTATAACGACAGCAAGGCAACCAATATTTTGGCTACTCAAAAAGCACTTTCTGGTTTTGATAATAG
TAAACTTTGGCTTTTAGCTGGCGGACTTGATCGTGGAAATGGTTTTGAAGAGTTAGAAAAAGATTTGCAAGATTTGAAAG
GAATGGTTGTTTTTGGGCAAACAGCAAATAAATTACGTTTGACTGCAGAAAAATTGAATATTCCCGTTTTTGATAGTGAA
AATGTTGCAAAAGCCCTCGAAGAAATTCTACCTCAAACGCAAGCAGGAGATACAATTTTACTTAGTCCAGCTTGTGCAAG
TTGGGACCAATATAAAACTTTTGAAGAACGCGGTGATTTATTCATTCAAGCCTTTGAAAATTTGAAAAAATAA

Upstream 100 bases:

>100_bases
AGTAAATAAGACTGTTGAAAAAAAGCCCGATTAGGGGTAAAATAGATTAGATAAAGTCGTCAGTGAAATATTTTACTGAC
AACTTAAAAAGGAATAGAAA

Downstream 100 bases:

>100_bases
AAGTACTGACAACTATTGAATTAATTTATGTTAAAATTAATTTTACTGACAGAAAGAATAATTACGATTCATCTTGCTGA
CAGCTATCAGATTTAATTTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQLLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALNIPVITEVELAYLISEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAEKASA
SDTLVMELSSFQLMGIDSFRPKIALITNLFSAHLDYHGSQKAYEAAKWRIQENMTSDDFLILNFNQEKCRNLADKTKATV
LAFSTKEKVNGAYSKDGKIYFNDEYIMEVSELSLPGEHNLENALAAIVASKLQGTKNEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEELEKDLQDLKGMVVFGQTANKLRLTAEKLNIPVFDSE
NVAKALEEILPQTQAGDTILLSPACASWDQYKTFEERGDLFIQAFENLKK

Sequences:

>Translated_450_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQLLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALNIPVITEVELAYLISEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAEKASA
SDTLVMELSSFQLMGIDSFRPKIALITNLFSAHLDYHGSQKAYEAAKWRIQENMTSDDFLILNFNQEKCRNLADKTKATV
LAFSTKEKVNGAYSKDGKIYFNDEYIMEVSELSLPGEHNLENALAAIVASKLQGTKNEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEELEKDLQDLKGMVVFGQTANKLRLTAEKLNIPVFDSE
NVAKALEEILPQTQAGDTILLSPACASWDQYKTFEERGDLFIQAFENLKK
>Mature_450_residues
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQLLLEEGIKVITGSHPIDLLDEDFALMVKNP
GIRYDNPMVERAEALNIPVITEVELAYLISEAPIIGITGTNGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAEKASA
SDTLVMELSSFQLMGIDSFRPKIALITNLFSAHLDYHGSQKAYEAAKWRIQENMTSDDFLILNFNQEKCRNLADKTKATV
LAFSTKEKVNGAYSKDGKIYFNDEYIMEVSELSLPGEHNLENALAAIVASKLQGTKNEAIVEVLTSFAGVKHRLQYLGEI
DGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGNGFEELEKDLQDLKGMVVFGQTANKLRLTAEKLNIPVFDSE
NVAKALEEILPQTQAGDTILLSPACASWDQYKTFEERGDLFIQAFENLKK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=457, Percent_Identity=31.2910284463895, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1790680, Length=355, Percent_Identity=26.1971830985915, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786279, Length=256, Percent_Identity=28.515625, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_LACLA (Q9CF91)

Other databases:

- EMBL:   AE005176
- PIR:   F86823
- RefSeq:   NP_267746.1
- ProteinModelPortal:   Q9CF91
- SMR:   Q9CF91
- GeneID:   1115250
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0237
- NMPDR:   fig|272623.1.peg.1632
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   LLAC272623:L0237-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49363; Mature: 49363

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQLLLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCEEE
TGSHPIDLLDEDFALMVKNPGIRYDNPMVERAEALNIPVITEVELAYLISEAPIIGITGT
ECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEEEEECC
NGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAEKASASDTLVMELSSFQLMGIDSFR
CCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEEEECCEEEECCCCCC
PKIALITNLFSAHLDYHGSQKAYEAAKWRIQENMTSDDFLILNFNQEKCRNLADKTKATV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEE
LAFSTKEKVNGAYSKDGKIYFNDEYIMEVSELSLPGEHNLENALAAIVASKLQGTKNEAI
EEEECHHHCCCCCCCCCEEEECCHHEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
VEVLTSFAGVKHRLQYLGEIDGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGN
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GFEELEKDLQDLKGMVVFGQTANKLRLTAEKLNIPVFDSENVAKALEEILPQTQAGDTIL
CHHHHHHHHHHHCCEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
LSPACASWDQYKTFEERGDLFIQAFENLKK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKITKFKDQKILVLGLARSGMAAALVLNELGAIVTVNDGKPFEENKEAQLLLEEGIKVI
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHCCCEEE
TGSHPIDLLDEDFALMVKNPGIRYDNPMVERAEALNIPVITEVELAYLISEAPIIGITGT
ECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHCCCCEEEEECC
NGKTTTTTLIADILNADGQSAKLSGNIGFPASEVAEKASASDTLVMELSSFQLMGIDSFR
CCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEEEECCEEEECCCCCC
PKIALITNLFSAHLDYHGSQKAYEAAKWRIQENMTSDDFLILNFNQEKCRNLADKTKATV
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEE
LAFSTKEKVNGAYSKDGKIYFNDEYIMEVSELSLPGEHNLENALAAIVASKLQGTKNEAI
EEEECHHHCCCCCCCCCEEEECCHHEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
VEVLTSFAGVKHRLQYLGEIDGRKVYNDSKATNILATQKALSGFDNSKLWLLAGGLDRGN
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GFEELEKDLQDLKGMVVFGQTANKLRLTAEKLNIPVFDSENVAKALEEILPQTQAGDTIL
CHHHHHHHHHHHCCEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEE
LSPACASWDQYKTFEERGDLFIQAFENLKK
ECCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471