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The map label for this gene is nagB

Identifier: 15673555

GI number: 15673555

Start: 1614659

End: 1615366

Strand: Reverse

Name: nagB

Synonym: L14408

Alternate gene names: 15673555

Gene position: 1615366-1614659 (Counterclockwise)

Preceding gene: 15673556

Following gene: 15673553

Centisome position: 68.29

GC content: 35.59

Gene sequence:

>708_bases
ATGAAAGTTATCGTAGTAAAAAATCAACTCGAAGGTGGAAAAATCGGACTTGACCTTCTTAAAAAATCAATGGCTAATGG
TGCTAAAACATTGGGACTTGCAACAGGCTCAACACCAGTAGAATTTTACAACCAAATTGTTAACTCAGACCTTGATTTTA
CAGACATGGTTTCAGTAAACTTGGATGAATATGTTGGACTTGACGGTTCAAATGATCAATCTTACCGCTATTTTATGACT
AAACATTTGTTTGGAGAAAAACCATTTAAAGAAAACTTCTTACCAAATGGTAAAGCAGCTGATCTTGAAGCTGAAGCAAA
ACATTATGACCAAATTATTGAAGAAAATCCAATTGACTGGCAAATTCTTGGAATTGGTCAAAATGGACACATTGGTTTTA
ATGAACCAGGAACACCTGCTGAAATTACAACTCATGTTGTTGACCTTCAAGAAAGCACAATCAAAGCCAACGCACGTTTC
TTTGAATCAGAAGCAGATGTACCACGTAAAGCAATTTCAATGGGACTTGCTTCAATTATGAAATCAAAAAATATTGTTTT
GATGGCTTATGGTAAAGAAAAAGCTGAAGCAATCAAAGGAATGGTTGAAGGAGAAGTGACAACAGAGCTTCCAGCATCAA
TTCTTCAAAACCACGCTAATGTTACTGTTATCGCTGATGAAGCAGCTGTATCATTGTTGAGCAAATAA

Upstream 100 bases:

>100_bases
ATTTTTTGAAGACAATTTTTAGTTGACATGGAGATAATTTATGATATAATGAAAGTGTAGAAAAATTGATATATACCAAT
TTAGAAATGGAGCATATAAA

Downstream 100 bases:

>100_bases
AATAAAAAAAAGTCCTGACAGGACTTTTTATTTACTGACAGAAATTCTGTCAGTATTTTTTTATCTTTTTTTATACTTAC
GGTGATTATCAACAACTTCT

Product: glucosamine-6-P isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKVIVVKNQLEGGKIGLDLLKKSMANGAKTLGLATGSTPVEFYNQIVNSDLDFTDMVSVNLDEYVGLDGSNDQSYRYFMT
KHLFGEKPFKENFLPNGKAADLEAEAKHYDQIIEENPIDWQILGIGQNGHIGFNEPGTPAEITTHVVDLQESTIKANARF
FESEADVPRKAISMGLASIMKSKNIVLMAYGKEKAEAIKGMVEGEVTTELPASILQNHANVTVIADEAAVSLLSK

Sequences:

>Translated_235_residues
MKVIVVKNQLEGGKIGLDLLKKSMANGAKTLGLATGSTPVEFYNQIVNSDLDFTDMVSVNLDEYVGLDGSNDQSYRYFMT
KHLFGEKPFKENFLPNGKAADLEAEAKHYDQIIEENPIDWQILGIGQNGHIGFNEPGTPAEITTHVVDLQESTIKANARF
FESEADVPRKAISMGLASIMKSKNIVLMAYGKEKAEAIKGMVEGEVTTELPASILQNHANVTVIADEAAVSLLSK
>Mature_235_residues
MKVIVVKNQLEGGKIGLDLLKKSMANGAKTLGLATGSTPVEFYNQIVNSDLDFTDMVSVNLDEYVGLDGSNDQSYRYFMT
KHLFGEKPFKENFLPNGKAADLEAEAKHYDQIIEENPIDWQILGIGQNGHIGFNEPGTPAEITTHVVDLQESTIKANARF
FESEADVPRKAISMGLASIMKSKNIVLMAYGKEKAEAIKGMVEGEVTTELPASILQNHANVTVIADEAAVSLLSK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=209, Percent_Identity=36.3636363636364, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI19923881, Length=208, Percent_Identity=34.6153846153846, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1786893, Length=216, Percent_Identity=32.8703703703704, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789530, Length=200, Percent_Identity=26.5, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI48994958, Length=237, Percent_Identity=25.3164556962025, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=211, Percent_Identity=36.4928909952607, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581960, Length=244, Percent_Identity=34.8360655737705, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19920764, Length=244, Percent_Identity=34.8360655737705, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_LACLA (Q9CFA8)

Other databases:

- EMBL:   AE005176
- PIR:   E86821
- RefSeq:   NP_267729.1
- ProteinModelPortal:   Q9CFA8
- SMR:   Q9CFA8
- GeneID:   1115233
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L14408
- NMPDR:   fig|272623.1.peg.1615
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   CLSK697812
- BioCyc:   LLAC272623:L14408-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 25620; Mature: 25620

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 62-62 ACT_SITE 128-128 ACT_SITE 130-130 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIVVKNQLEGGKIGLDLLKKSMANGAKTLGLATGSTPVEFYNQIVNSDLDFTDMVSVN
CEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHEEEEC
LDEYVGLDGSNDQSYRYFMTKHLFGEKPFKENFLPNGKAADLEAEAKHYDQIIEENPIDW
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCE
QILGIGQNGHIGFNEPGTPAEITTHVVDLQESTIKANARFFESEADVPRKAISMGLASIM
EEEEECCCCCCCCCCCCCCHHHHHEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KSKNIVLMAYGKEKAEAIKGMVEGEVTTELPASILQNHANVTVIADEAAVSLLSK
CCCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHCC
>Mature Secondary Structure
MKVIVVKNQLEGGKIGLDLLKKSMANGAKTLGLATGSTPVEFYNQIVNSDLDFTDMVSVN
CEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHEEEEC
LDEYVGLDGSNDQSYRYFMTKHLFGEKPFKENFLPNGKAADLEAEAKHYDQIIEENPIDW
HHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCE
QILGIGQNGHIGFNEPGTPAEITTHVVDLQESTIKANARFFESEADVPRKAISMGLASIM
EEEEECCCCCCCCCCCCCCHHHHHEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
KSKNIVLMAYGKEKAEAIKGMVEGEVTTELPASILQNHANVTVIADEAAVSLLSK
CCCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471