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The map label for this gene is purD

Identifier: 15673495

GI number: 15673495

Start: 1553256

End: 1554497

Strand: Reverse

Name: purD

Synonym: L153005

Alternate gene names: 15673495

Gene position: 1554497-1553256 (Counterclockwise)

Preceding gene: 15673499

Following gene: 15673494

Centisome position: 65.71

GC content: 38.0

Gene sequence:

>1242_bases
ATGATGAAAATTTTGGTAATTGGTTCTGGCGGCCGCGAACATGCCTTAGCAAAAAAATTTATGGAAAGTCCTCAAGTTGA
AGAAGTCTTTGTAGCTCCAGGCAATTCAGGAATGGAAAAAGATGGAATTCAAATAGTTGATATTTCCGAACTATCTAATG
ATAAATTAGTTAAATTTGCTCAAAATCAAAACATTGGACTAACATTTGTTGGGCCAGAAACAGCTTTGATGAATGGCGTC
GTCGATGCTTTTATAAAAGCAGAATTACCAATTTTTGGGCCAAATAAAATGGCCGCAGAGCTAGAAGGTTCTAAAGATTT
TGCAAAATCAATTATGAAAAAATATGGGGTTCCGACGGCTGATTATGCTACTTTTGATAGCTTAGAGCCTGCTCTTGCAT
ATCTAGATGAAAAAGGAGTTCCTCTTGTGATTAAAGCTGATGGGCTCGCTGCTGGAAAGGGTGTGACGGTTGCCTTTGAT
ATTGAGACCGCTAAGTCAGCTCTGGCCGATATTTTTTCTGGTAGTCAAGGAAAAGTTGTGATTGAGGAATTTCTTGATGG
TGAAGAATTTTCTTTATTTAGTTTTATCCATGACGGGAAAATATATCCGATGCCGATTGCTCAAGACCACAAACGAGCTT
TTGATGGAGATAAAGGCCCTAACACAGGGGGAATGGGGGCTTACTCACCGGTCCTACATATTTCTAAGGAGGTTGTAAAT
GAGGCTCTGGAAAAAGTTGTTAAACCAACAGTAGCTGGAATGATAGAAGAAGGCAAGTCATTTACAGGCGTTCTTTATGC
AGGTTTAATTTTGACAGAAGACGGAGTCAAAACAATTGAATTTAATGCACGCTTTGGGGATCCTGAAACCCAAGTTGTTC
TCCCCCGTTTAAAAAGTGACTTAGCTCAAGCAATTATTGATATTTTAGCTGGAAATGAGCCAACATTAGAATGGCTTGAG
TCTGGTGTGACTTTGGGTGTGGTTGTTGCTGCAGAAGGCTATCCTAGTCAAGCAAAACTAGGACTTATTCTTCCAGAAAT
TCCTGAAGGTTTAAATGTTTATTATGCAGGAGTTTCCAAAAATGAAAATAATCAATTAATTTCTAGTGGAGGCCGAGTTT
ATCTTGTTTCTGAAACGGGAGAAGATGTAAAAAGTACACAAAAATTGCTTTATGAAAAACTTGATAAATTGGAAAATGAT
GGTTTCTTTTATCGTCATGATATTGGTTCACGAGCAATATAA

Upstream 100 bases:

>100_bases
CTTTGACTTTTATTATTCGTTCAAGATTTGTTGAATAATAAATAAAATAGCTGAATACACAAGTCTGTGTATATAAAAAG
CGTTTGGGAATATCGGAGAA

Downstream 100 bases:

>100_bases
AAATTTTGGTTAAGAAAAAAAGAAGAGGAAAAATGGCAGAAGTAGCAATTATCATGGGTTCGAGCTCAGATTGGGCGACA
ATGAAAGAAACAGCAAAAAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MMKILVIGSGGREHALAKKFMESPQVEEVFVAPGNSGMEKDGIQIVDISELSNDKLVKFAQNQNIGLTFVGPETALMNGV
VDAFIKAELPIFGPNKMAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFD
IETAKSALADIFSGSQGKVVIEEFLDGEEFSLFSFIHDGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVLHISKEVVN
EALEKVVKPTVAGMIEEGKSFTGVLYAGLILTEDGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGNEPTLEWLE
SGVTLGVVVAAEGYPSQAKLGLILPEIPEGLNVYYAGVSKNENNQLISSGGRVYLVSETGEDVKSTQKLLYEKLDKLEND
GFFYRHDIGSRAI

Sequences:

>Translated_413_residues
MMKILVIGSGGREHALAKKFMESPQVEEVFVAPGNSGMEKDGIQIVDISELSNDKLVKFAQNQNIGLTFVGPETALMNGV
VDAFIKAELPIFGPNKMAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFD
IETAKSALADIFSGSQGKVVIEEFLDGEEFSLFSFIHDGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVLHISKEVVN
EALEKVVKPTVAGMIEEGKSFTGVLYAGLILTEDGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGNEPTLEWLE
SGVTLGVVVAAEGYPSQAKLGLILPEIPEGLNVYYAGVSKNENNQLISSGGRVYLVSETGEDVKSTQKLLYEKLDKLEND
GFFYRHDIGSRAI
>Mature_413_residues
MMKILVIGSGGREHALAKKFMESPQVEEVFVAPGNSGMEKDGIQIVDISELSNDKLVKFAQNQNIGLTFVGPETALMNGV
VDAFIKAELPIFGPNKMAAELEGSKDFAKSIMKKYGVPTADYATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFD
IETAKSALADIFSGSQGKVVIEEFLDGEEFSLFSFIHDGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVLHISKEVVN
EALEKVVKPTVAGMIEEGKSFTGVLYAGLILTEDGVKTIEFNARFGDPETQVVLPRLKSDLAQAIIDILAGNEPTLEWLE
SGVTLGVVVAAEGYPSQAKLGLILPEIPEGLNVYYAGVSKNENNQLISSGGRVYLVSETGEDVKSTQKLLYEKLDKLEND
GFFYRHDIGSRAI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=43.1603773584906, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=43.1603773584906, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=43.1603773584906, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=43.1603773584906, Blast_Score=320, Evalue=1e-87,
Organism=Escherichia coli, GI1790438, Length=425, Percent_Identity=48.4705882352941, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=37.5886524822695, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=38.9140271493213, Blast_Score=285, Evalue=7e-78,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=37.5, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=37.5, Blast_Score=260, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44420; Mature: 44420

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKILVIGSGGREHALAKKFMESPQVEEVFVAPGNSGMEKDGIQIVDISELSNDKLVKFA
CEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEHHHCCCCCEEEEE
QNQNIGLTFVGPETALMNGVVDAFIKAELPIFGPNKMAAELEGSKDFAKSIMKKYGVPTA
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
DYATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFDIETAKSALADIFSGSQGKVV
CCCCHHCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEE
IEEFLDGEEFSLFSFIHDGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVLHISKEVVN
HHHHCCCCCEEEEEEEECCEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
EALEKVVKPTVAGMIEEGKSFTGVLYAGLILTEDGVKTIEFNARFGDPETQVVLPRLKSD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCCEEEHHHHHHH
LAQAIIDILAGNEPTLEWLESGVTLGVVVAAEGYPSQAKLGLILPEIPEGLNVYYAGVSK
HHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCC
NENNQLISSGGRVYLVSETGEDVKSTQKLLYEKLDKLENDGFFYRHDIGSRAI
CCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MMKILVIGSGGREHALAKKFMESPQVEEVFVAPGNSGMEKDGIQIVDISELSNDKLVKFA
CEEEEEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEHHHCCCCCEEEEE
QNQNIGLTFVGPETALMNGVVDAFIKAELPIFGPNKMAAELEGSKDFAKSIMKKYGVPTA
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCC
DYATFDSLEPALAYLDEKGVPLVIKADGLAAGKGVTVAFDIETAKSALADIFSGSQGKVV
CCCCHHCCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEE
IEEFLDGEEFSLFSFIHDGKIYPMPIAQDHKRAFDGDKGPNTGGMGAYSPVLHISKEVVN
HHHHCCCCCEEEEEEEECCEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
EALEKVVKPTVAGMIEEGKSFTGVLYAGLILTEDGVKTIEFNARFGDPETQVVLPRLKSD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCCEEEHHHHHHH
LAQAIIDILAGNEPTLEWLESGVTLGVVVAAEGYPSQAKLGLILPEIPEGLNVYYAGVSK
HHHHHHHHHCCCCCHHHHHHCCCEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCC
NENNQLISSGGRVYLVSETGEDVKSTQKLLYEKLDKLENDGFFYRHDIGSRAI
CCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797284; 11337471 [H]