Click here to switch to the map view.

The map label for this gene is purK

Identifier: 15673493

GI number: 15673493

Start: 1551581

End: 1552630

Strand: Reverse

Name: purK

Synonym: L151330

Alternate gene names: 15673493

Gene position: 1552630-1551581 (Counterclockwise)

Preceding gene: 15673494

Following gene: 15673491

Centisome position: 65.63

GC content: 36.57

Gene sequence:

>1050_bases
ATGATGGCAATTGCTGCTCAATACATGGGACATAAAGTCATTACACTTGACCCAAATCCAAACTGTTCTGCGGCAAAAGT
ATCTGATGAACTGATTGTTGCACCTTATGATGACGTTGAAAATTTATTAAGACTGGCCTATGCTTGTGACGTCATTACTT
ATGAGTTTGAAAATGTATCAGCAAAAGCTTTGCACGAAATTGAAGGCTGTGTAAGGATTCCTCAAGGAATACGTCTGTTA
GAGATTACGCAAAACCGTCGCTTTGAAAAAGAATTTTTGACCAACGAAGCTAAAGTTAATGTTGCACCTTGGCAACTCGT
AGATTCAGCGGAAAAACTCCCAGAAACAGTAACTAGAAAACAAGTTTTAAAAACAACAACTGGAGGCTATGACGGTCATG
GACAAGTGGTTTTAAATACTGATGAAAAATTGTCAGCTGCAAAATCACTGACAGAACTTTCAGAATGTGTTTTAGAAGAT
TTTATTTCTTTTGAACGTGAAATTTCTGTTATTATTAGTGGAAATGGTCACGAATATGTGGTTTTTCCTCTAGCTGAAAA
CGAACATAGAGAAAACATCTTACATCAAACGATTTCTCCGGCTCGTATATCTGCTGAAATTACAGAGAATGCTTATAAAA
TTGCGACCTCAATTGCGGAAAAATTAGAGCTATCTGGCGTGCTTTGTGTGGAAATGTTCTTAACAGCAGATGGTCAAATC
TATGTCAATGAACTAGCACCAAGACCACATAATAGTGGCCACTTTACCATTGAAGCTTGTGATTTCAACCAATTTGATTT
GCATATTAAAGGAATTTTAGGAGAAGACCTACCAGAACCTAAACTTTTGAAACCTGCCATTATGCTGAACGTTTTAGGGC
AACACGTTGAGGCAGTTAAGAAACTTAATCATGAGCATGCCGATTGGCATCAACATGATTATGGAAAAGCAGATGCTAAA
CATAACCGAAAAATGGGACATGTTACGATCTTAACAAATGATTTTGACCAAACGATTAATGAAATTAAAGCAACTAAAAT
TTGGGATTAA

Upstream 100 bases:

>100_bases
TCAAAAGTTGTCGGATTTCAAATAATGAAGGGTAGGAAGACTTGTGATAAAGAATACAAAGCAAACAATTGGTATTATTG
GTGGAGGTCAGCTTGGACAG

Downstream 100 bases:

>100_bases
TAAAAATACTTGGTAAAAGCCAAGTATTTTTTTTATTTGATTTAGGCATGATAAAATGACAACATTTCCCCTGAAAAATA
GAACAAAACAGTAAGATAAG

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MMAIAAQYMGHKVITLDPNPNCSAAKVSDELIVAPYDDVENLLRLAYACDVITYEFENVSAKALHEIEGCVRIPQGIRLL
EITQNRRFEKEFLTNEAKVNVAPWQLVDSAEKLPETVTRKQVLKTTTGGYDGHGQVVLNTDEKLSAAKSLTELSECVLED
FISFEREISVIISGNGHEYVVFPLAENEHRENILHQTISPARISAEITENAYKIATSIAEKLELSGVLCVEMFLTADGQI
YVNELAPRPHNSGHFTIEACDFNQFDLHIKGILGEDLPEPKLLKPAIMLNVLGQHVEAVKKLNHEHADWHQHDYGKADAK
HNRKMGHVTILTNDFDQTINEIKATKIWD

Sequences:

>Translated_349_residues
MMAIAAQYMGHKVITLDPNPNCSAAKVSDELIVAPYDDVENLLRLAYACDVITYEFENVSAKALHEIEGCVRIPQGIRLL
EITQNRRFEKEFLTNEAKVNVAPWQLVDSAEKLPETVTRKQVLKTTTGGYDGHGQVVLNTDEKLSAAKSLTELSECVLED
FISFEREISVIISGNGHEYVVFPLAENEHRENILHQTISPARISAEITENAYKIATSIAEKLELSGVLCVEMFLTADGQI
YVNELAPRPHNSGHFTIEACDFNQFDLHIKGILGEDLPEPKLLKPAIMLNVLGQHVEAVKKLNHEHADWHQHDYGKADAK
HNRKMGHVTILTNDFDQTINEIKATKIWD
>Mature_349_residues
MMAIAAQYMGHKVITLDPNPNCSAAKVSDELIVAPYDDVENLLRLAYACDVITYEFENVSAKALHEIEGCVRIPQGIRLL
EITQNRRFEKEFLTNEAKVNVAPWQLVDSAEKLPETVTRKQVLKTTTGGYDGHGQVVLNTDEKLSAAKSLTELSECVLED
FISFEREISVIISGNGHEYVVFPLAENEHRENILHQTISPARISAEITENAYKIATSIAEKLELSGVLCVEMFLTADGQI
YVNELAPRPHNSGHFTIEACDFNQFDLHIKGILGEDLPEPKLLKPAIMLNVLGQHVEAVKKLNHEHADWHQHDYGKADAK
HNRKMGHVTILTNDFDQTINEIKATKIWD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=276, Percent_Identity=31.8840579710145, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788155, Length=351, Percent_Identity=23.6467236467236, Blast_Score=78, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=371, Percent_Identity=30.4582210242588, Blast_Score=139, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006140
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C; PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39160; Mature: 39160

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAIAAQYMGHKVITLDPNPNCSAAKVSDELIVAPYDDVENLLRLAYACDVITYEFENVS
CCEEEEHHHCCEEEEECCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHEEEECCCCC
AKALHEIEGCVRIPQGIRLLEITQNRRFEKEFLTNEAKVNVAPWQLVDSAEKLPETVTRK
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
QVLKTTTGGYDGHGQVVLNTDEKLSAAKSLTELSECVLEDFISFEREISVIISGNGHEYV
HHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
VFPLAENEHRENILHQTISPARISAEITENAYKIATSIAEKLELSGVLCVEMFLTADGQI
EEECCCCCHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCEE
YVNELAPRPHNSGHFTIEACDFNQFDLHIKGILGEDLPEPKLLKPAIMLNVLGQHVEAVK
EEEECCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
KLNHEHADWHQHDYGKADAKHNRKMGHVTILTNDFDQTINEIKATKIWD
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMAIAAQYMGHKVITLDPNPNCSAAKVSDELIVAPYDDVENLLRLAYACDVITYEFENVS
CCEEEEHHHCCEEEEECCCCCCCCEECCCCEEEECHHHHHHHHHHHHHHHHEEEECCCCC
AKALHEIEGCVRIPQGIRLLEITQNRRFEKEFLTNEAKVNVAPWQLVDSAEKLPETVTRK
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
QVLKTTTGGYDGHGQVVLNTDEKLSAAKSLTELSECVLEDFISFEREISVIISGNGHEYV
HHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEE
VFPLAENEHRENILHQTISPARISAEITENAYKIATSIAEKLELSGVLCVEMFLTADGQI
EEECCCCCHHHHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCEE
YVNELAPRPHNSGHFTIEACDFNQFDLHIKGILGEDLPEPKLLKPAIMLNVLGQHVEAVK
EEEECCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
KLNHEHADWHQHDYGKADAKHNRKMGHVTILTNDFDQTINEIKATKIWD
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA