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The map label for this gene is busAB

Identifier: 15673433

GI number: 15673433

Start: 1472729

End: 1474450

Strand: Reverse

Name: busAB

Synonym: L72477

Alternate gene names: 15673433

Gene position: 1474450-1472729 (Counterclockwise)

Preceding gene: 15673434

Following gene: 15673432

Centisome position: 62.33

GC content: 36.53

Gene sequence:

>1722_bases
ATGATTGATTTAGCTATTGGACAAGTACCCATAGCAAACTGGGTGTCATCGGCAACAGATTGGATTACTAGTACTTTTAG
TTCAGGATTTGATGTGATTCAAAAATCAGGAACGGTATTGATGAACGGTATTACAGGTGCTTTAACTGCTGTTCCATTCT
GGTTGATGATTGCTGTTGTTACTATCCTTGCCATTTTGGTTTCAGGTAAAAAAATTGCCTTTCCTTTATTCACTTTCATC
GGTCTTTCTTTAATTGCTAACCAGGGCTTATGGTCAGATTTGATGAGTACAATCACACTTGTCTTACTTTCAAGTTTATT
GTCAATCATCATTGGAGTTCCTTTAGGGATTTGGATGGCCAAATCTGATTTAGTGGCTAAGATCGTTCAACCGATTTTGG
ACTTTATGCAAACGATGCCTGGTTTCGTTTATTTGATTCCAGCGGTTGCATTCTTTGGAATAGGAGTTGTTCCTGGGGTA
TTTGCATCTGTTATCTTTGCACTCCCACCAACAGTCAGAATGACTAACTTGGGAATTCGTCAAGTTTCAACGGAATTAGT
TGAAGCGGCAGATTCATTTGGTTCAACTGCCCGTCAAAAGCTTTTTAAATTGGAATTTCCTTTGGCTAAAGGAACAATTA
TGGCTGGTGTTAACCAAACGATTATGTTAGCTCTTTCTATGGTTGTTATCGCTTCTATGATTGGGGCGCCTGGCTTAGGT
CGTGGTGTACTTGCAGCTGTTCAATCGGCTGATATTGGTAAAGGGTTTGTAAGTGGGATTTCATTAGTTATTTTGGCTAT
TATTATTGACCGCTTTACACAAAAACTTAATGTTTCTCCTCTTGAAAAACAGGGAAATCCTACAGTTAAAAAATGGAAAC
GTGGAATTGCCTTAGTTTCATTGTTAGCTTTAATTATTGGTGCCTTTTCAGGAATGTCTTTTGGGAAAACAGCCTCTGAT
AAAAAAGTTGACTTAGTTTATATGAACTGGGATTCAGAAGTTGCATCAATCAATGTTTTGACTCAAGCGATGAAAGAACA
CGGCTTTGATGTTAAAACGACAGCTTTAGATAATGCGGTGGCTTGGCAAACAGTAGCCAATGGTCAAGCAGATGGAATGG
TTTCTGCTTGGTTACCAAACACGCATAAAACACAATGGCAAAAATATGGTAAATCTGTTGATTTGTTAGGTCCTAACCTT
AAAGGGGCTAAAGTTGGGTTTGTTGTTCCAAGTTATATGAATGTTAATTCAATTGAAGATTTAACAAATCAAGCGAATAA
AACAATCACAGGTATTGAACCAGGTGCAGGTGTAATGGCTGCTAGTGAAAAAACGCTTAATAGCTATGATAATCTTAAAG
ATTGGAAACTTGTTCCTTCATCATCAGGTGCAATGACTGTAGCTCTTGGTGAAGCAATCAAACAACATAAAGATATTGTC
ATTACTGGTTGGTCTCCTCATTGGATGTTTAACAAATATGATTTGAAATATTTGGCTGATCCAAAAGGAACAATGGGAAC
TTCAGAAAATATTAATACCATTGTTCGTAAAGGTTTGAAGAAAGAAAATCCAGAAGCTTATAAAGTTTTAGATAAATTTA
ATTGGACAACTAAGGATATGGAAGCTGTCATGCTTGATATTCAAAATGGTAAAACACCAGAAGAAGCTGCCAAAAATTGG
ATTAAAGATCATCAAAAAGAAGTTGATAAATGGTTTAAATAA

Upstream 100 bases:

>100_bases
TCCTTAAAGGTGTATTGATTCGAGGAAGTGTTTTAGAGGCCTTAGCTGATATTCCTGATGAGGACGAAGTAGAGGAGATT
GAAAAAGAGGAGGAGAATAA

Downstream 100 bases:

>100_bases
TCAACGAAGATTTAGATTTAATGAAAACTTTCTAGCATTATAGTTAGAAGGTTTTTTATTATTTCATTCTAAGTTTATTA
TTTTTAGATTTATAACATTT

Product: betaine ABC transporter permease and substrate binding protein

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MIDLAIGQVPIANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKIAFPLFTFI
GLSLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSDLVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPTVKKWKRGIALVSLLALIIGAFSGMSFGKTASD
KKVDLVYMNWDSEVASINVLTQAMKEHGFDVKTTALDNAVAWQTVANGQADGMVSAWLPNTHKTQWQKYGKSVDLLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASEKTLNSYDNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWMFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLDKFNWTTKDMEAVMLDIQNGKTPEEAAKNW
IKDHQKEVDKWFK

Sequences:

>Translated_573_residues
MIDLAIGQVPIANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKIAFPLFTFI
GLSLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSDLVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPTVKKWKRGIALVSLLALIIGAFSGMSFGKTASD
KKVDLVYMNWDSEVASINVLTQAMKEHGFDVKTTALDNAVAWQTVANGQADGMVSAWLPNTHKTQWQKYGKSVDLLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASEKTLNSYDNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWMFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLDKFNWTTKDMEAVMLDIQNGKTPEEAAKNW
IKDHQKEVDKWFK
>Mature_573_residues
MIDLAIGQVPIANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVVTILAILVSGKKIAFPLFTFI
GLSLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMAKSDLVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGV
FASVIFALPPTVRMTNLGIRQVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPTVKKWKRGIALVSLLALIIGAFSGMSFGKTASD
KKVDLVYMNWDSEVASINVLTQAMKEHGFDVKTTALDNAVAWQTVANGQADGMVSAWLPNTHKTQWQKYGKSVDLLGPNL
KGAKVGFVVPSYMNVNSIEDLTNQANKTITGIEPGAGVMAASEKTLNSYDNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
ITGWSPHWMFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLDKFNWTTKDMEAVMLDIQNGKTPEEAAKNW
IKDHQKEVDKWFK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG2113

COG function: function code E; ABC-type proline/glycine betaine transport systems, periplasmic components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=284, Percent_Identity=46.830985915493, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1788451, Length=193, Percent_Identity=29.0155440414508, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788449, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1786564, Length=175, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 61952; Mature: 61952

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLAIGQVPIANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
TILAILVSGKKIAFPLFTFIGLSLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSDLVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGVFASVIFALPPTVRMTNLGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCHHH
QVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPTVKKWKRGIALVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LLALIIGAFSGMSFGKTASDKKVDLVYMNWDSEVASINVLTQAMKEHGFDVKTTALDNAV
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCHH
AWQTVANGQADGMVSAWLPNTHKTQWQKYGKSVDLLGPNLKGAKVGFVVPSYMNVNSIED
HHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCEECCCCCCCCCEEEEEECCCCCCHHHHH
LTNQANKTITGIEPGAGVMAASEKTLNSYDNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
HHHHHCCEECCCCCCCCCEECCHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHCCCEE
ITGWSPHWMFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLDKFNWTTKDM
EECCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHH
EAVMLDIQNGKTPEEAAKNWIKDHQKEVDKWFK
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDLAIGQVPIANWVSSATDWITSTFSSGFDVIQKSGTVLMNGITGALTAVPFWLMIAVV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHH
TILAILVSGKKIAFPLFTFIGLSLIANQGLWSDLMSTITLVLLSSLLSIIIGVPLGIWMA
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
KSDLVAKIVQPILDFMQTMPGFVYLIPAVAFFGIGVVPGVFASVIFALPPTVRMTNLGIR
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHCHHH
QVSTELVEAADSFGSTARQKLFKLEFPLAKGTIMAGVNQTIMLALSMVVIASMIGAPGLG
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RGVLAAVQSADIGKGFVSGISLVILAIIIDRFTQKLNVSPLEKQGNPTVKKWKRGIALVS
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LLALIIGAFSGMSFGKTASDKKVDLVYMNWDSEVASINVLTQAMKEHGFDVKTTALDNAV
HHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHCCHH
AWQTVANGQADGMVSAWLPNTHKTQWQKYGKSVDLLGPNLKGAKVGFVVPSYMNVNSIED
HHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCEECCCCCCCCCEEEEEECCCCCCHHHHH
LTNQANKTITGIEPGAGVMAASEKTLNSYDNLKDWKLVPSSSGAMTVALGEAIKQHKDIV
HHHHHCCEECCCCCCCCCEECCHHHHHHHCCCCCCEECCCCCCCEEHHHHHHHHHCCCEE
ITGWSPHWMFNKYDLKYLADPKGTMGTSENINTIVRKGLKKENPEAYKVLDKFNWTTKDM
EECCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHH
EAVMLDIQNGKTPEEAAKNWIKDHQKEVDKWFK
HHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]