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The map label for this gene is pi348

Identifier: 15673403

GI number: 15673403

Start: 1449659

End: 1450084

Strand: Reverse

Name: pi348

Synonym: L0301

Alternate gene names: 15673403

Gene position: 1450084-1449659 (Counterclockwise)

Preceding gene: 15673404

Following gene: 15673402

Centisome position: 61.3

GC content: 35.45

Gene sequence:

>426_bases
ATGATTAACAATGTCACTCTAGTAGGGCGAATTACTAAAGAACCTGAACTTAGATATACACCACAAAATAAAGCAGTTGC
CACTTTTACTCTTGCAGTTAATCGAGCATTTAAAAATGCTAATGGAGAAAGAGAAGCTGACTTTATCAATTGTGTTATTT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATCGGAGTTACTGGTAGTATTCAAACTCGA
AACTACGAGAACCAACAAGGTCAACGAGTTTATGTAACAGAAGTCATTGCAAACAATTTCCAAGTACTTGAAAAAAGCAA
TCAAGCAAATGGTGAACGAGTTAGTAATCCAGCTGCAAAACCACAAAATAATGATTCTTTTGGAAGTGATCCAATGGAAA
TTTCAGATGATGACCTACCATTTTAA

Upstream 100 bases:

>100_bases
GGGGCAAGTGTTGATGAACAAAATGATTGGCTAAAAATGCCACTTGATGAAGCATACAATGATATCGAAAAATTCGTAGA
TATAAAAAAGGAAGAACAAA

Downstream 100 bases:

>100_bases
TTAACAACCAGGTGCAGCGTGCGTAACAAATGCTTAAATTCGAGGGGATAGGCAATGCGCAACATCCCCCAGCCTTTAAT
TTGAAAAATAAAACTTGAAA

Product: single strand binding helix destabilising protein

Products: NA

Alternate protein names: SSB 2; Helix-destabilizing protein 2 [H]

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYVTEVIANNFQVLEKSNQANGERVSNPAAKPQNNDSFGSDPMEISDDDLPF

Sequences:

>Translated_141_residues
MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYVTEVIANNFQVLEKSNQANGERVSNPAAKPQNNDSFGSDPMEISDDDLPF
>Mature_141_residues
MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENQQGQRVYVTEVIANNFQVLEKSNQANGERVSNPAAKPQNNDSFGSDPMEISDDDLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Escherichia coli, GI1790494, Length=100, Percent_Identity=37, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 15702; Mature: 15702

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGEREADFINCVIWGKSAEN
CCCCEEEEEEECCCCCEEECCCCCEEEEEEEEEEHHHHCCCCCCCCCEEEEEEECCCHHH
LANWTHKGQLIGVTGSIQTRNYENQQGQRVYVTEVIANNFQVLEKSNQANGERVSNPAAK
HHCCCCCCEEEEEECCEEECCCCCCCCCEEEEEEEHHCCEEEEECCCCCCCCCCCCCCCC
PQNNDSFGSDPMEISDDDLPF
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MINNVTLVGRITKEPELRYTPQNKAVATFTLAVNRAFKNANGEREADFINCVIWGKSAEN
CCCCEEEEEEECCCCCEEECCCCCEEEEEEEEEEHHHHCCCCCCCCCEEEEEEECCCHHH
LANWTHKGQLIGVTGSIQTRNYENQQGQRVYVTEVIANNFQVLEKSNQANGERVSNPAAK
HHCCCCCCEEEEEECCEEECCCCCCCCCEEEEEEEHHCCEEEEECCCCCCCCCCCCCCCC
PQNNDSFGSDPMEISDDDLPF
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]