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The map label for this gene is pyrF

Identifier: 15673327

GI number: 15673327

Start: 1381946

End: 1382659

Strand: Reverse

Name: pyrF

Synonym: L181692

Alternate gene names: 15673327

Gene position: 1382659-1381946 (Counterclockwise)

Preceding gene: 15673328

Following gene: 15673326

Centisome position: 58.45

GC content: 40.9

Gene sequence:

>714_bases
ATGCAAGAAAATAGACCTGTCATTGCCCTTGATTTCCCTGAATTCTCAGACGTAAAAGATTTTCTCGAAAAATTTGATCC
GTCAGAACAATTGTATATTAAACTAGGAATGGAACTTTTTTACACGGCTGGACCCCAAGTCGTTTACTATGTAAAATCGC
TCGGCCACAGTGTATTCCTTGATTTAAAACTCCATGATATTCCAAACACCGTTGAATCCTCAATGCATGTTTTAGCACGT
TTGGGAGTGGATATGGTTAATGTTCACGCCGCTGGTGGTGTTGAAATGATGGTCGCGGCTAAACGCGGTTTAGAGGCTGG
AACGCCAGTTGGACGGCAAAGGCCAAAATTAATTGCTGTCACACAATTAACCTCAACTTCTGAGGAAATTATGCAAAATG
ACCAAAAAATTATGACTAGTCTTGAAGAATCGGTTATTAATTACGCACAAAAAACCGCTCAAGCAGGACTTGACGGTGTC
GTTTGTTCGGCACATGAAGTTGAAAAAATTAAAGCAGCGACATCGAAAGAATTCATTTGTCTCACACCGGGAATTCGCCC
AGAAGGTGCAAGTAAAGGCGACCAAAAACGAGTAATGACACCTAAAGAAGCAAGAACAATTGGTTCAGATTATATTGTTG
TCGGCCGTCCAATTACCCAAGCAAAAGATCCAGTAGCTAGCTATCATGCGATAAAAGCAGAATGGAATCGATAA

Upstream 100 bases:

>100_bases
TAAAATGCGACCAAAGCGAGCATTTTATGGATAGCTAAAAGAATTGTCAGCGGAGCTGATAATTCTGTCATTAAATATTT
AGAAAAAGGAAGTAGAAAAA

Downstream 100 bases:

>100_bases
AATGAAAAAATTACTTCACTTTTTGGTTGTGTTACTTGGAATATTTGTAGCTTTTATACTGGCTCGATTATTCATAGAGA
TTTATACGATTGTTGGTGAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MQENRPVIALDFPEFSDVKDFLEKFDPSEQLYIKLGMELFYTAGPQVVYYVKSLGHSVFLDLKLHDIPNTVESSMHVLAR
LGVDMVNVHAAGGVEMMVAAKRGLEAGTPVGRQRPKLIAVTQLTSTSEEIMQNDQKIMTSLEESVINYAQKTAQAGLDGV
VCSAHEVEKIKAATSKEFICLTPGIRPEGASKGDQKRVMTPKEARTIGSDYIVVGRPITQAKDPVASYHAIKAEWNR

Sequences:

>Translated_237_residues
MQENRPVIALDFPEFSDVKDFLEKFDPSEQLYIKLGMELFYTAGPQVVYYVKSLGHSVFLDLKLHDIPNTVESSMHVLAR
LGVDMVNVHAAGGVEMMVAAKRGLEAGTPVGRQRPKLIAVTQLTSTSEEIMQNDQKIMTSLEESVINYAQKTAQAGLDGV
VCSAHEVEKIKAATSKEFICLTPGIRPEGASKGDQKRVMTPKEARTIGSDYIVVGRPITQAKDPVASYHAIKAEWNR
>Mature_237_residues
MQENRPVIALDFPEFSDVKDFLEKFDPSEQLYIKLGMELFYTAGPQVVYYVKSLGHSVFLDLKLHDIPNTVESSMHVLAR
LGVDMVNVHAAGGVEMMVAAKRGLEAGTPVGRQRPKLIAVTQLTSTSEEIMQNDQKIMTSLEESVINYAQKTAQAGLDGV
VCSAHEVEKIKAATSKEFICLTPGIRPEGASKGDQKRVMTPKEARTIGSDYIVVGRPITQAKDPVASYHAIKAEWNR

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=239, Percent_Identity=45.1882845188285, Blast_Score=196, Evalue=1e-51,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_LACLA (Q9CFW9)

Other databases:

- EMBL:   AF174425
- EMBL:   AE005176
- PIR:   A86793
- RefSeq:   NP_267501.1
- ProteinModelPortal:   Q9CFW9
- SMR:   Q9CFW9
- GeneID:   1114995
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L181692
- NMPDR:   fig|272623.1.peg.1380
- HOGENOM:   HBG625253
- OMA:   RTIGSDY
- ProtClustDB:   PRK00230
- BioCyc:   LLAC272623:L181692-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26106; Mature: 26106

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 63-63 BINDING 11-11 BINDING 34-34 BINDING 124-124 BINDING 186-186 BINDING 195-195 BINDING 215-215 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENRPVIALDFPEFSDVKDFLEKFDPSEQLYIKLGMELFYTAGPQVVYYVKSLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECHHHEECCCCCEEHHHHHCCCEEEE
DLKLHDIPNTVESSMHVLARLGVDMVNVHAAGGVEMMVAAKRGLEAGTPVGRQRPKLIAV
EEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCEEEE
TQLTSTSEEIMQNDQKIMTSLEESVINYAQKTAQAGLDGVVCSAHEVEKIKAATSKEFIC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCEEE
LTPGIRPEGASKGDQKRVMTPKEARTIGSDYIVVGRPITQAKDPVASYHAIKAEWNR
ECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHEEEECCCC
>Mature Secondary Structure
MQENRPVIALDFPEFSDVKDFLEKFDPSEQLYIKLGMELFYTAGPQVVYYVKSLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEEEECHHHEECCCCCEEHHHHHCCCEEEE
DLKLHDIPNTVESSMHVLARLGVDMVNVHAAGGVEMMVAAKRGLEAGTPVGRQRPKLIAV
EEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCCCCCCCCCCCCCCEEEE
TQLTSTSEEIMQNDQKIMTSLEESVINYAQKTAQAGLDGVVCSAHEVEKIKAATSKEFIC
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCEEE
LTPGIRPEGASKGDQKRVMTPKEARTIGSDYIVVGRPITQAKDPVASYHAIKAEWNR
ECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10742196; 11337471