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The map label for this gene is thiE

Identifier: 15673244

GI number: 15673244

Start: 1293610

End: 1294257

Strand: Reverse

Name: thiE

Synonym: L0201

Alternate gene names: 15673244

Gene position: 1294257-1293610 (Counterclockwise)

Preceding gene: 15673245

Following gene: 15673243

Centisome position: 54.71

GC content: 35.8

Gene sequence:

>648_bases
ATGAAAAATAAAATTTTAGACCTGAGGGCATATTTTATTGCTGGCCCACAAGATTTTCCAAAACTTTCAATTGATGATGC
AATCGATAAAATTTCTGTAATCATAAAAAGTGGAGTAACGGTTTATCAATTTCGTGATAAGGGAACAATTTATAAAAATA
AAAATCAACGATTAGAAGTAGCAAAAAGACTACAAGAAGTAGCTCAGAAAGCGGCTGTTTCTTTTATTGTGAATGATGAT
GTTGAATTAGCGCGTGAATTGTCAGCTGACGGGATTCATGTCGGACAAGACGATGATTCTGTCAGTAAAATTCGTGAGCT
GATTGGCCAAGAAATGTGGGTAGGACTTTCTGTCAGTAATGATATGGAATTAGAAAGCGCTCAAAAGAGTGGGGCTGACT
ATTTGGGAATTGGTCCAATTTATCCAACAAATAGCAAGTCCGACGCAGCAGAACCAATTGGGGTTGACCATTTAAGAAAA
ATGCTTGAGCATAATCAATTACCAACTGTTGGAATTGGTGGAATTACTGAAAATTCACTGACAGAGCTTTCAAAAATTGG
TCTGGGTGGAGTTGCGGTAATTTCTTTGCTGACAGAATCCGAAAATTACAAAAATATGGTTCAAAAAATTAAGCAAAATA
TTAGATGA

Upstream 100 bases:

>100_bases
GAAGTGATGCCAAAAGCTAAATTGTTTATTACTGAAGCTATTTCACAAACAATCAATGTTGGTCACGGTCACGGACCGTT
AAACCATTGGGTGACAATTA

Downstream 100 bases:

>100_bases
AAAAGATGTTGTACCCTTAAAAATACAACAAAACAAAATGATTCCGTTTTCTTTTTATTATATAAATTAAACTTACACAA
ATGTAAGTTTTTTTTCTTTA

Product: thiamin-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKNKILDLRAYFIAGPQDFPKLSIDDAIDKISVIIKSGVTVYQFRDKGTIYKNKNQRLEVAKRLQEVAQKAAVSFIVNDD
VELARELSADGIHVGQDDDSVSKIRELIGQEMWVGLSVSNDMELESAQKSGADYLGIGPIYPTNSKSDAAEPIGVDHLRK
MLEHNQLPTVGIGGITENSLTELSKIGLGGVAVISLLTESENYKNMVQKIKQNIR

Sequences:

>Translated_215_residues
MKNKILDLRAYFIAGPQDFPKLSIDDAIDKISVIIKSGVTVYQFRDKGTIYKNKNQRLEVAKRLQEVAQKAAVSFIVNDD
VELARELSADGIHVGQDDDSVSKIRELIGQEMWVGLSVSNDMELESAQKSGADYLGIGPIYPTNSKSDAAEPIGVDHLRK
MLEHNQLPTVGIGGITENSLTELSKIGLGGVAVISLLTESENYKNMVQKIKQNIR
>Mature_215_residues
MKNKILDLRAYFIAGPQDFPKLSIDDAIDKISVIIKSGVTVYQFRDKGTIYKNKNQRLEVAKRLQEVAQKAAVSFIVNDD
VELARELSADGIHVGQDDDSVSKIRELIGQEMWVGLSVSNDMELESAQKSGADYLGIGPIYPTNSKSDAAEPIGVDHLRK
MLEHNQLPTVGIGGITENSLTELSKIGLGGVAVISLLTESENYKNMVQKIKQNIR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=197, Percent_Identity=25.8883248730964, Blast_Score=80, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325042, Length=152, Percent_Identity=34.8684210526316, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LACLA (Q9CG48)

Other databases:

- EMBL:   AE005176
- PIR:   F86782
- RefSeq:   NP_267418.1
- ProteinModelPortal:   Q9CG48
- SMR:   Q9CG48
- GeneID:   1114911
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0201
- NMPDR:   fig|272623.1.peg.1297
- HOGENOM:   HBG754477
- OMA:   CERYKTR
- ProtClustDB:   CLSK697654
- BioCyc:   LLAC272623:L0201-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23657; Mature: 23657

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKILDLRAYFIAGPQDFPKLSIDDAIDKISVIIKSGVTVYQFRDKGTIYKNKNQRLEV
CCCCCEEHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCHHHHH
AKRLQEVAQKAAVSFIVNDDVELARELSADGIHVGQDDDSVSKIRELIGQEMWVGLSVSN
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHEEEEECCC
DMELESAQKSGADYLGIGPIYPTNSKSDAAEPIGVDHLRKMLEHNQLPTVGIGGITENSL
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHH
TELSKIGLGGVAVISLLTESENYKNMVQKIKQNIR
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNKILDLRAYFIAGPQDFPKLSIDDAIDKISVIIKSGVTVYQFRDKGTIYKNKNQRLEV
CCCCCEEHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEECCCCHHHHH
AKRLQEVAQKAAVSFIVNDDVELARELSADGIHVGQDDDSVSKIRELIGQEMWVGLSVSN
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHEEEEECCC
DMELESAQKSGADYLGIGPIYPTNSKSDAAEPIGVDHLRKMLEHNQLPTVGIGGITENSL
CCHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHH
TELSKIGLGGVAVISLLTESENYKNMVQKIKQNIR
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471