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The map label for this gene is pmrA

Identifier: 15672648

GI number: 15672648

Start: 660575

End: 661792

Strand: Direct

Name: pmrA

Synonym: L60571

Alternate gene names: 15672648

Gene position: 660575-661792 (Clockwise)

Preceding gene: 15672647

Following gene: 15672649

Centisome position: 27.92

GC content: 38.51

Gene sequence:

>1218_bases
GTGAATCGCACGAAAACAGTTAATTGGAAAAGGAATTTATTTATAACATGGATTGGCTGCTTTTTTGTAGGTTCATCATT
TTCACTCGTCATGCCCTTTCTCCCCTTGTATATTCAAGGACTGGGTGTAAGCGGTGGGAATGTTGAACTTTACTCAGGGT
TAGCTTTTTCTTTGCCAGCCTTAGCTTCAGGGTTAGTTGCCCCCATTTGGGGACGTCTAGCTGATGAGCATGGGCGTAAA
GTGATGATGGTACGTGCCAGTATCGTCATGACCCTAACAATGGGAGGAATAGCTTTTGCCCCTAATGTTTGGTGGTTACT
TGGATTACGTTTGCTAATGGGATTTTTCTCAGGCTATATCCCTAACTCAACAGCAATGATTGCTTCACAAGCTCCAAAAG
ATAAATCAGGCTATGCCCTTGGAACATTAGCAACAGCAATGGTTTCTGGAACACTGATTGGTCCTTCACTTGGTGGGTTA
TTAGCTGAGTGGTTTGGCATGGCAAATGTCTTTTTGATTGTCGGCGCTCTATTAGCCTTAGCAACTTTACTAACCATTTT
CTTTGTTCATGAAAACTTTGAACCAATTGCTAAAGGTGAAATGTTATCTAGTAAAGAAATTATTAATAAAGTCAGTAACA
AACAAATTCTTTTTGGATTATTAGTTACAACATTTATTATTCAAATCACCTCGCAATCCATTGAACCATTTGTGACCTTG
TATATCAAAACCCTGACGACAAGCACGAACAATTTGATGTTCATTTCAGGTTTAATTGTGTCAGCAGTTGGATTGTCGGC
AATGTTATCATCAAGTTTTCTAGGACGTTTAGGAGATAAATACGGTTCACATCGTTTAATTTTGATTGGACTAGTTTTTA
CATTTATTATTTATTTACCAATGGCCTTTGTGCAAAGCCCACTCCAACTTGGGATATTACGCTTCCTTCTTGGTTTTGGA
ACTGGTGCTTTGACGCCGTCAGTAAATTCGTTGCTTTCAAAAATTACTCCCAAAGAAGGTGTCAGCAGAATTTTTGCTTA
CGCACAAATGTGTTCTAATTTAGGAATGGTAACCGGTCCATTAGTCGGATCAGCAATTGCAGGTTATATCAGTTATCGAG
CAGCAATTGTAGGGACAAGTCTTTTTGTCATTGTTAATATTATTTGGTCCTTTATTAATTTCCGAAAATATCTGCGGAAA
CGGAGTATTATGGAATGA

Upstream 100 bases:

>100_bases
AAAAAGTCGCTGATGTCATTCTGGATAATTTTGGAACGATTGAAGCACTAAAAAAACAAATCCAGCGAGAACTAGCTAGG
ATAGAAGAACAGAAATAGAG

Downstream 100 bases:

>100_bases
GAGTAAAAATTACATTAATTTGTAGTTCTTGTGGGAACAAAAATTATATTAGTAGCAAAAATAAAGCAACCCATCCGGAA
AAAGTTGAAACAATGAAGTT

Product: multidrug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNRTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
LAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSKEIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMLSSSFLGRLGDKYGSHRLILIGLVFTFIIYLPMAFVQSPLQLGILRFLLGFG
TGALTPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME

Sequences:

>Translated_405_residues
MNRTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
LAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSKEIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMLSSSFLGRLGDKYGSHRLILIGLVFTFIIYLPMAFVQSPLQLGILRFLLGFG
TGALTPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME
>Mature_405_residues
MNRTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRK
VMMVRASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYIPNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGL
LAEWFGMANVFLIVGALLALATLLTIFFVHENFEPIAKGEMLSSKEIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
YIKTLTTSTNNLMFISGLIVSAVGLSAMLSSSFLGRLGDKYGSHRLILIGLVFTFIIYLPMAFVQSPLQLGILRFLLGFG
TGALTPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGPLVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRK
RSIME

Specific function: Efflux pump for various substrates

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family

Homologues:

Organism=Escherichia coli, GI1787291, Length=400, Percent_Identity=44.5, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=25.5102040816327, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PMRA_LACLA (P58120)

Other databases:

- EMBL:   AE005176
- PIR:   B86708
- RefSeq:   NP_266822.1
- ProteinModelPortal:   P58120
- GeneID:   1114290
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L60571
- NMPDR:   fig|272623.1.peg.684
- HOGENOM:   HBG404070
- OMA:   HENFEPI
- ProtClustDB:   CLSK697341
- BioCyc:   LLAC272623:L60571-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958
- PRINTS:   PR01035

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 43860; Mature: 43860

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd643a84)-; HASH(0xcbdb3f0)-; HASH(0xcd661b4)-; HASH(0xc8bc954)-; HASH(0xd50a050)-; HASH(0xd6a7e50)-; HASH(0xd2f4f10)-; HASH(0xd828f20)-; HASH(0xc0d2aec)-; HASH(0xcdf0c1c)-; HASH(0xcea55f0)-; HASH(0xd6c8098)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPA
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
LASGLVAPIWGRLADEHGRKVMMVRASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYI
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCC
PNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGLLAEWFGMANVFLIVGALLAL
CCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATLLTIFFVHENFEPIAKGEMLSSKEIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
HHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
YIKTLTTSTNNLMFISGLIVSAVGLSAMLSSSFLGRLGDKYGSHRLILIGLVFTFIIYLP
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAFVQSPLQLGILRFLLGFGTGALTPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGP
HHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRKRSIME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MNRTKTVNWKRNLFITWIGCFFVGSSFSLVMPFLPLYIQGLGVSGGNVELYSGLAFSLPA
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHH
LASGLVAPIWGRLADEHGRKVMMVRASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYI
HHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHCCC
PNSTAMIASQAPKDKSGYALGTLATAMVSGTLIGPSLGGLLAEWFGMANVFLIVGALLAL
CCCCEEHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ATLLTIFFVHENFEPIAKGEMLSSKEIINKVSNKQILFGLLVTTFIIQITSQSIEPFVTL
HHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
YIKTLTTSTNNLMFISGLIVSAVGLSAMLSSSFLGRLGDKYGSHRLILIGLVFTFIIYLP
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
MAFVQSPLQLGILRFLLGFGTGALTPSVNSLLSKITPKEGVSRIFAYAQMCSNLGMVTGP
HHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
LVGSAIAGYISYRAAIVGTSLFVIVNIIWSFINFRKYLRKRSIME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11337471