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The map label for this gene is pfl

Identifier: 15672646

GI number: 15672646

Start: 657418

End: 659781

Strand: Direct

Name: pfl

Synonym: L57408

Alternate gene names: 15672646

Gene position: 657418-659781 (Clockwise)

Preceding gene: 15672645

Following gene: 15672647

Centisome position: 27.79

GC content: 37.9

Gene sequence:

>2364_bases
ATGAAAACCGAAGTTACGGAAAATATCTTTGAACAAGCTTGGGATGGTTTTAAAGGAACCAACTGGCGCGATAAAGCAAG
CGTTACTCGCTTTGTACAAGAAAACTACAAACCATATGATGGTGATGAAAGCTTTCTTGCTGGGCCAACAGAACGTACAC
TTAAAGTAAAGAAAATTATTGAAGATACAAAAAATCACTACGAAGAAGTAGGATTTCCCTTCGATACTGACCGCGTAACC
TCTATTGATAAAATCCCTGCTGGATATATCGATGCTAATGATAAAGAACTTGAACTCATCTATGGGATGCAAAATAGCGA
ACTTTTCCGCTTGAATTTCATGCCAAGAGGTGGACTTCGTGTTGCTGAAAAGATTTTGACAGAACACGGTCTCTCAGTTG
ACCCAGGCTTGCATGATGTTTTGTCACAAACAATGACTTCTGTAAATGATGGAATCTTTCGTGCTTATACTTCAGCAATT
CGTAAAGCACGTCATGCTCATACTGTAACAGGTTTGCCAGATGCTTACTCTCGTGGACGTATCATTGGTGTCTATGCACG
TCTTGCCCTTTACGGTGCTGATTACCTTATGAAGGAAAAAGCAAAAGAATGGGATGCAATCACTGAAATTAACGAAGAAA
ACATTCGTCTTAAAGAAGAAATTAATATGCAATACCAAGCTTTGCAAGAAGTTGTAAACTTTGGTGCTTTATATGGTCTT
GATGTTTCACGTCCAGCTATGAACGTAAAAGAAGCAATCCAATGGGTTAACATCGCTTATATGGCAGTATGTCGTGTCAT
TAATGGAGCTGCAACTTCACTTGGACGTGTTCCAATCGTTCTTGATATCTTTGCAGAACGTGACCTTGCTCGTGGAACAT
TTACTGAACAAGAAATTCAAGAATTTGTTGATGATTTCGTTTTGAAGCTTCGTACAATGAAATTTGCGCGTGCAGCTGCT
TATGATGAACTTTATTCTGGTGACCCAACATTCATCACAACATCTATGGCTGGTATGGGTAATGACGGACGTCACCGTGT
CACTAAAATGGACTACCGTTTCTTGAACACACTTGATACAATCGGAAATGCTCCAGAACCAAACTTGACAGTCCTTTGGG
ATTCTAAACTTCCTTACTCATTCAAACGTTATTCAATGTCTATGAGCCACAAGCATTCTTCTATTCAATATGAAGGTGTT
GAAACAATGGCTAAAGATGGATATGGCGAAATGTCATGTATCTCTTGTTGTGTCTCACCACTTGATCCAGAAAATGAAGA
AGGACGTCATAACCTCCAATACTTTGGTGCGCGTGTAAACGTCTTGAAAGCAATGTTGACTGGTTTGAACGGTGGTTATG
ATGACGTTCATAAAGATTATAAAGTATTCGACATCGAACCTGTTCGTGACGAAATTCTTGACTATGATACAGTTATGGAA
AACTTTGACAAATCTCTCGACTGGTTGACTGATACTTATGTTGATGCAATGAATATCATTCATTACATGACTGATAAATA
TAACTATGAAGCAGTTCAAATGGCCTTCTTGCCTACTAAAGTTCGTGCTAACATGGGATTTGGTATCTGTGGATTCGCAA
ATACAGTTGATTCACTTTCAGCAATTAAATATGCTAAAGTTAAAACATTGCGTGATGAAAATGGCTATATCTACGATTAC
GAAGTAGAAGGTGATTTCCCTCGTTATGGTGAAGATGATGATCGTGCTGATGATATTGCTAAACTTGTCATGAAAATGTA
CCATGAAAAATTAGCTTCACACAAACTTTACAAAAATGCTGAAGCTACTGTTTCACTTTTGACAATTACATCTAACGTTG
CTTACTCTAAACAAACTGGTAATTCTCCAGTACATAAAGGAGTATTCCTCAATGAAGATGGTACAGTAAATAAATCTAAA
CTTGAATTCTTCTCACCAGGTGCTAACCCATCTAATAAAGCTAAGGGTGGTTGGTTGCAAAATCTTCGCTCATTGGCTAA
GTTGGAATTCAAAGATGCAAATGATGGTATTTCATTGACTACTCAAGTTTCACCTCGTGCACTTGGTAAAACTCGTGATG
AACAAGTGGATAACTTGGTTCAAATTCTTGATGGATACTTCACACCAGGTGCTTTGATTAATGGTACTGAATTTGCAGGT
CAACACGTTAACTTGAACGTAATGGACCTTAAAGATGTTTACGATAAAATCATGCGTGGTGAAGATGTTATCGTTCGTAT
CTCTGGTTACTGTGTCAATACTAAATACCTCACACCAGAACAAAAACAAGAATTAACTGAACGTGTCTTCCATGAAGTTC
TTTCAAACGATGATGAAGAAGTAATGCATACTTCAAACATCTAA

Upstream 100 bases:

>100_bases
AAAGAATCTGTAATTTCTCTTGAATTCTGTTTGCTATTCTCAAACTGTATGATATAATGAAGTTGCAATTTGAAACAGAA
AGAACAAAGGAGATTTCAAA

Downstream 100 bases:

>100_bases
TTCTTAAAATTTAATGAATATTCGGTCTGTCAGTTTTACTGACAGACTTTTTTTACGAAAAAATTAATCATAATAGTTAA
AAACTATTGTTTTTAGTTTA

Product: pyruvate-formate lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT
SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI
RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINEENIRLKEEINMQYQALQEVVNFGALYGL
DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFVLKLRTMKFARAAA
YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV
ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME
NFDKSLDWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY
EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK
LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG
QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI

Sequences:

>Translated_787_residues
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT
SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI
RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINEENIRLKEEINMQYQALQEVVNFGALYGL
DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFVLKLRTMKFARAAA
YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV
ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME
NFDKSLDWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY
EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK
LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG
QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI
>Mature_787_residues
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKIIEDTKNHYEEVGFPFDTDRVT
SIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLRVAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAI
RKARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMKEKAKEWDAITEINEENIRLKEEINMQYQALQEVVNFGALYGL
DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQEFVDDFVLKLRTMKFARAAA
YDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDTIGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGV
ETMAKDGYGEMSCISCCVSPLDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME
NFDKSLDWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLSAIKYAKVKTLRDENGYIYDY
EVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNAEATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSK
LEFFSPGANPSNKAKGGWLQNLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG
QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEEVMHTSNI

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain

Homologues:

Organism=Escherichia coli, GI1787131, Length=774, Percent_Identity=43.0232558139535, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI48994926, Length=788, Percent_Identity=40.8629441624365, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PFL_LACLA (O32797)

Other databases:

- EMBL:   AJ000326
- EMBL:   AE005176
- PIR:   H86707
- RefSeq:   NP_266820.1
- ProteinModelPortal:   O32797
- GeneID:   1114288
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L57408
- NMPDR:   fig|272623.1.peg.682
- HOGENOM:   HBG285825
- OMA:   KYAKVKT
- ProtClustDB:   CLSK869953
- BioCyc:   LLAC272623:L57408-MONOMER
- BRENDA:   2.3.1.54
- GO:   GO:0005737
- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- PIRSF:   PIRSF000379
- TIGRFAMs:   TIGR01255

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL

EC number: =2.3.1.54

Molecular weight: Translated: 89123; Mature: 89123

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: ACT_SITE 416-416 ACT_SITE 417-417

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKII
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
EDTKNHYEEVGFPFDTDRVTSIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLR
HHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHH
VAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAIRKARHAHTVTGLPDAYSRGR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
IIGVYARLALYGADYLMKEKAKEWDAITEINEENIRLKEEINMQYQALQEVVNFGALYGL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCC
DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHCCCCCHHHHH
EFVDDFVLKLRTMKFARAAAYDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
IGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGVETMAKDGYGEMSCISCCVSP
HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHCCC
LDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHH
NFDKSLDWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCEECCHHHHHHHHH
AIKYAKVKTLRDENGYIYDYEVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNA
HHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSKLEFFSPGANPSNKAKGGWLQ
CCEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCHHHH
NLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG
HHHHHHHEECCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCC
QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEE
CEEEEEEEEHHHHHHHHHCCCCEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHCCCCHH
VMHTSNI
HHHCCCC
>Mature Secondary Structure
MKTEVTENIFEQAWDGFKGTNWRDKASVTRFVQENYKPYDGDESFLAGPTERTLKVKKII
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
EDTKNHYEEVGFPFDTDRVTSIDKIPAGYIDANDKELELIYGMQNSELFRLNFMPRGGLR
HHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHH
VAEKILTEHGLSVDPGLHDVLSQTMTSVNDGIFRAYTSAIRKARHAHTVTGLPDAYSRGR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCC
IIGVYARLALYGADYLMKEKAKEWDAITEINEENIRLKEEINMQYQALQEVVNFGALYGL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHCCC
DVSRPAMNVKEAIQWVNIAYMAVCRVINGAATSLGRVPIVLDIFAERDLARGTFTEQEIQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHCCCHHCCCCCHHHHH
EFVDDFVLKLRTMKFARAAAYDELYSGDPTFITTSMAGMGNDGRHRVTKMDYRFLNTLDT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
IGNAPEPNLTVLWDSKLPYSFKRYSMSMSHKHSSIQYEGVETMAKDGYGEMSCISCCVSP
HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCHHHHHHHCCC
LDPENEEGRHNLQYFGARVNVLKAMLTGLNGGYDDVHKDYKVFDIEPVRDEILDYDTVME
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHH
NFDKSLDWLTDTYVDAMNIIHYMTDKYNYEAVQMAFLPTKVRANMGFGICGFANTVDSLS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHCCCCCEECCHHHHHHHHH
AIKYAKVKTLRDENGYIYDYEVEGDFPRYGEDDDRADDIAKLVMKMYHEKLASHKLYKNA
HHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EATVSLLTITSNVAYSKQTGNSPVHKGVFLNEDGTVNKSKLEFFSPGANPSNKAKGGWLQ
CCEEEEEEEECCCEECCCCCCCCCCCCEEECCCCCCCCCHHEEECCCCCCCCCCCCHHHH
NLRSLAKLEFKDANDGISLTTQVSPRALGKTRDEQVDNLVQILDGYFTPGALINGTEFAG
HHHHHHHEECCCCCCCEEEEEECCCHHHCCCHHHHHHHHHHHHHCCCCCCCEECCHHHCC
QHVNLNVMDLKDVYDKIMRGEDVIVRISGYCVNTKYLTPEQKQELTERVFHEVLSNDDEE
CEEEEEEEEHHHHHHHHHCCCCEEEEEECEEEECCCCCCHHHHHHHHHHHHHHHCCCCHH
VMHTSNI
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294449; 11337471