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The map label for this gene is eno

Identifier: 15672626

GI number: 15672626

Start: 633126

End: 634427

Strand: Direct

Name: eno

Synonym: L0007

Alternate gene names: 15672626

Gene position: 633126-634427 (Clockwise)

Preceding gene: 15672625

Following gene: 15672627

Centisome position: 26.76

GC content: 42.63

Gene sequence:

>1302_bases
ATGTCAATTATTACTGATATTTATGCTCGCGAAGTCCTTGACTCACGCGGTAACCCAACACTTGAAGTTGAAGTTTACAC
TGAAGACGGTGCATTCGGTCGCGGTATGGTACCTTCAGGTGCTTCTACTGGTGAACATGAAGCGGTTGAACTTCGTGATG
GCGACAAATCTCGCTACAACGGACTTGGTACTCAAAAAGCTGTTGACAACGTAAACAACATCATCGCTGAAGCTATCATC
GGTTATGAAGTTACTGACCAACAAGCTATTGACCGTGCAATGATCGCTCTTGACGGTACTGAAAACAAAGGTAAATTGGG
AGCTAACGCTATCCTTGGTGTTTCTATCGCTGCTGCTCGTGCTGCTGCTGATGAACTTGGTGTTCCACTTTACAACTACC
TTGGCGGATTCAACGCTAAAGTATTGCCAACTCCAATGATGAACATCATCAATGGTGGTTCTCACTCAGACGCTCCAATC
GCTTTCCAAGAATTCATGATCGTACCAGTTGGTGCACCTACATTCAAAGAAGCGCTTCGTTGGGGTGCTGAAATCTTCCA
CGCTCTTAAGAAAATTCTTAAAGCTCGTGGACTTGAAACAGCTGTCGGTGACGAAGGTGGATTCGCTCCTAAATTCGACG
GAACTGAAGACGGTGTAGAAACTATCCTTAAAGCAATCGAAGCAGCTGGTTACAAAGCTGGTGAAGATGGCGTTATGATC
GGTTTCGACTGTGCATCATCAGAATTCTACGAAAACGGTGTTTACGACTACACTAAATTCGAAGGTGAAGGCGGTAAAAA
ACTTTCAGCTTCTGAACAAGTTGACTACCTTGAAGAACTCGTTTCTAAATACCCAATCATCACTATTGAAGATGGTATGG
ACGAAAACGACTGGGATGGATGGAAAATCCTTACTGAACGTCTTGGTAAAAAAGTTCAACTCGTTGGTGACGACTTCTTC
GTTACAAACACTAAATACCTTGAACGTGGTATCCGTGAAAATGCTTCAAACGCTATCTTGATCAAAGTTAACCAAATCGG
TACTTTGACAGAAACTTTCGAAGCTATTGAAATGGCTAAAGAAGCTGGTTTCACAGCAATCGTTTCTCACCGTTCTGGTG
AAACTGAAGATTCAACAATCTCAGATATCGCTGTTGCAACTAACGCTGGTCAAATCAAAACTGGTTCACTTTCACGTACA
GACCGTATGGCTAAATACAACCAATTGCTTCGTATCGAAGACCAATTGGCTGAAGTGGCTCAATACAAAGGTCTTAAAGC
ATTCTACAACCTTAAAAAATAA

Upstream 100 bases:

>100_bases
AGCTTTGCGAAGATTTTCACTTGAAAAATCTTTCAAAAAATAGTAAAATCAAAGATGTATTAAGAGTGCAGACGCACTTA
AAAATAATAAGGAGACTAAA

Downstream 100 bases:

>100_bases
GGTTTAGATGGTTTTAATTAGAAATATCTGAATTTAATAAATGTCCTTTCTATGTTTAATTACAAAACATAGGGAGGGCA
TTTTTTGATGCAATTCGAAC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSIITDIYAREVLDSRGNPTLEVEVYTEDGAFGRGMVPSGASTGEHEAVELRDGDKSRYNGLGTQKAVDNVNNIIAEAII
GYEVTDQQAIDRAMIALDGTENKGKLGANAILGVSIAAARAAADELGVPLYNYLGGFNAKVLPTPMMNIINGGSHSDAPI
AFQEFMIVPVGAPTFKEALRWGAEIFHALKKILKARGLETAVGDEGGFAPKFDGTEDGVETILKAIEAAGYKAGEDGVMI
GFDCASSEFYENGVYDYTKFEGEGGKKLSASEQVDYLEELVSKYPIITIEDGMDENDWDGWKILTERLGKKVQLVGDDFF
VTNTKYLERGIRENASNAILIKVNQIGTLTETFEAIEMAKEAGFTAIVSHRSGETEDSTISDIAVATNAGQIKTGSLSRT
DRMAKYNQLLRIEDQLAEVAQYKGLKAFYNLKK

Sequences:

>Translated_433_residues
MSIITDIYAREVLDSRGNPTLEVEVYTEDGAFGRGMVPSGASTGEHEAVELRDGDKSRYNGLGTQKAVDNVNNIIAEAII
GYEVTDQQAIDRAMIALDGTENKGKLGANAILGVSIAAARAAADELGVPLYNYLGGFNAKVLPTPMMNIINGGSHSDAPI
AFQEFMIVPVGAPTFKEALRWGAEIFHALKKILKARGLETAVGDEGGFAPKFDGTEDGVETILKAIEAAGYKAGEDGVMI
GFDCASSEFYENGVYDYTKFEGEGGKKLSASEQVDYLEELVSKYPIITIEDGMDENDWDGWKILTERLGKKVQLVGDDFF
VTNTKYLERGIRENASNAILIKVNQIGTLTETFEAIEMAKEAGFTAIVSHRSGETEDSTISDIAVATNAGQIKTGSLSRT
DRMAKYNQLLRIEDQLAEVAQYKGLKAFYNLKK
>Mature_432_residues
SIITDIYAREVLDSRGNPTLEVEVYTEDGAFGRGMVPSGASTGEHEAVELRDGDKSRYNGLGTQKAVDNVNNIIAEAIIG
YEVTDQQAIDRAMIALDGTENKGKLGANAILGVSIAAARAAADELGVPLYNYLGGFNAKVLPTPMMNIINGGSHSDAPIA
FQEFMIVPVGAPTFKEALRWGAEIFHALKKILKARGLETAVGDEGGFAPKFDGTEDGVETILKAIEAAGYKAGEDGVMIG
FDCASSEFYENGVYDYTKFEGEGGKKLSASEQVDYLEELVSKYPIITIEDGMDENDWDGWKILTERLGKKVQLVGDDFFV
TNTKYLERGIRENASNAILIKVNQIGTLTETFEAIEMAKEAGFTAIVSHRSGETEDSTISDIAVATNAGQIKTGSLSRTD
RMAKYNQLLRIEDQLAEVAQYKGLKAFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=438, Percent_Identity=49.0867579908676, Blast_Score=413, Evalue=1e-115,
Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=49.4172494172494, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=49.4279176201373, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=49.4279176201373, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=435, Percent_Identity=45.0574712643678, Blast_Score=355, Evalue=6e-98,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=25.5131964809384, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=25.5131964809384, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=25.5131964809384, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=57.906976744186, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=50.4651162790698, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=50.4651162790698, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=297, Percent_Identity=38.7205387205387, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=48.8479262672811, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=48.8479262672811, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=48.8479262672811, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.8845265588915, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.1154734411085, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=48.735632183908, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=48.735632183908, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=48.735632183908, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=48.735632183908, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=48.735632183908, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=48.735632183908, Blast_Score=379, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO1_LACLA (Q9CHS7)

Other databases:

- EMBL:   AE005176
- PIR:   D86705
- RefSeq:   NP_266800.1
- ProteinModelPortal:   Q9CHS7
- SMR:   Q9CHS7
- GeneID:   1114264
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0007
- NMPDR:   fig|272623.1.peg.659
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   LLAC272623:L0007-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46912; Mature: 46781

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 342-342 BINDING 155-155 BINDING 164-164 BINDING 290-290 BINDING 317-317 BINDING 342-342 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIITDIYAREVLDSRGNPTLEVEVYTEDGAFGRGMVPSGASTGEHEAVELRDGDKSRYN
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHC
GLGTQKAVDNVNNIIAEAIIGYEVTDQQAIDRAMIALDGTENKGKLGANAILGVSIAAAR
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHH
AAADELGVPLYNYLGGFNAKVLPTPMMNIINGGSHSDAPIAFQEFMIVPVGAPTFKEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHH
WGAEIFHALKKILKARGLETAVGDEGGFAPKFDGTEDGVETILKAIEAAGYKAGEDGVMI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
GFDCASSEFYENGVYDYTKFEGEGGKKLSASEQVDYLEELVSKYPIITIEDGMDENDWDG
EEECCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
WKILTERLGKKVQLVGDDFFVTNTKYLERGIRENASNAILIKVNQIGTLTETFEAIEMAK
HHHHHHHCCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
EAGFTAIVSHRSGETEDSTISDIAVATNAGQIKTGSLSRTDRMAKYNQLLRIEDQLAEVA
HCCCEEEEECCCCCCCCCCHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QYKGLKAFYNLKK
HHCCCHHHHCCCC
>Mature Secondary Structure 
SIITDIYAREVLDSRGNPTLEVEVYTEDGAFGRGMVPSGASTGEHEAVELRDGDKSRYN
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHC
GLGTQKAVDNVNNIIAEAIIGYEVTDQQAIDRAMIALDGTENKGKLGANAILGVSIAAAR
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHH
AAADELGVPLYNYLGGFNAKVLPTPMMNIINGGSHSDAPIAFQEFMIVPVGAPTFKEALR
HHHHHHCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEEEEECCCHHHHHHHH
WGAEIFHALKKILKARGLETAVGDEGGFAPKFDGTEDGVETILKAIEAAGYKAGEDGVMI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEE
GFDCASSEFYENGVYDYTKFEGEGGKKLSASEQVDYLEELVSKYPIITIEDGMDENDWDG
EEECCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCH
WKILTERLGKKVQLVGDDFFVTNTKYLERGIRENASNAILIKVNQIGTLTETFEAIEMAK
HHHHHHHCCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
EAGFTAIVSHRSGETEDSTISDIAVATNAGQIKTGSLSRTDRMAKYNQLLRIEDQLAEVA
HCCCEEEEECCCCCCCCCCHHHEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
QYKGLKAFYNLKK
HHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471