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The map label for this gene is greA

Identifier: 15672618

GI number: 15672618

Start: 625972

End: 626442

Strand: Direct

Name: greA

Synonym: L0140

Alternate gene names: 15672618

Gene position: 625972-626442 (Clockwise)

Preceding gene: 15672617

Following gene: 15672623

Centisome position: 26.46

GC content: 35.46

Gene sequence:

>471_bases
ATGGAAAAAACATTCCCAATGACCCAAGAAGGGCTTGATAAATTAAAATTAGAACTTGAAAATTTAAAACTTGTGAAACG
TCCAGAAGTCATTGACCGTATCAAAGTTGCTCGTAGCTATGGTGACCTTTCAGAAAACTCAGAGTACGAAGCAGCTAAAG
ATGAACAAGCCTTTATCGAAGGACGTATTTCAACTGTTGAAACAATGATTCGTTATGCTGAAATTGTTGATAACGCAAAA
ATTGCTAAAGACGAAGTAGCACTTGGTAAAAACGTAACTTTTGTTGAAGTTGGCGAAACTGACGAAGAATCTTATCAAAT
TGTTGGTACTGCTGAAGCTGATCCATTTTCAGGAAAAATTTCTAATGAATCACCAATTGCTCGAGTTTTGATTGGAAAAA
AAGTTGGAGACATCGTAAATGTACCACTTCCAGTTGGCGAAATGACAGTTAAAATTGTAAAAGTTGACTAA

Upstream 100 bases:

>100_bases
ATGTTCAAAAATATGTGAATGATAAGATAAGTAGCTAAGCTATCATTAATTTAGATTTGATTAAAAGAGAAAAAAGTTTA
TAAAAAAGGAAAGAAATATA

Downstream 100 bases:

>100_bases
TTATTTTAGAATATAATAAGTAAAAAAGCCGTCAGTTGATGTGCACCCCAAAAGTTAGACTTTTTATCCAAGTAGAAATG
GAAAGTCAACGAAAAACTAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MEKTFPMTQEGLDKLKLELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFSGKISNESPIARVLIGKKVGDIVNVPLPVGEMTVKIVKVD

Sequences:

>Translated_156_residues
MEKTFPMTQEGLDKLKLELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFSGKISNESPIARVLIGKKVGDIVNVPLPVGEMTVKIVKVD
>Mature_156_residues
MEKTFPMTQEGLDKLKLELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIEGRISTVETMIRYAEIVDNAK
IAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFSGKISNESPIARVLIGKKVGDIVNVPLPVGEMTVKIVKVD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=155, Percent_Identity=43.2258064516129, Blast_Score=123, Evalue=5e-30,
Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=34.8684210526316, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_LACLA (Q9CHT2)

Other databases:

- EMBL:   AE005176
- PIR:   D86704
- RefSeq:   NP_266792.1
- ProteinModelPortal:   Q9CHT2
- SMR:   Q9CHT2
- GeneID:   1114256
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0140
- NMPDR:   fig|272623.1.peg.651
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   LLAC272623:L0140-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17274; Mature: 17274

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTFPMTQEGLDKLKLELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIE
CCCCCCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
GRISTVETMIRYAEIVDNAKIAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFSGKI
HHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCC
SNESPIARVLIGKKVGDIVNVPLPVGEMTVKIVKVD
CCCCCHHHHHHCCCCCCEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MEKTFPMTQEGLDKLKLELENLKLVKRPEVIDRIKVARSYGDLSENSEYEAAKDEQAFIE
CCCCCCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
GRISTVETMIRYAEIVDNAKIAKDEVALGKNVTFVEVGETDEESYQIVGTAEADPFSGKI
HHHHHHHHHHHHHHHHCCCHHCHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCC
SNESPIARVLIGKKVGDIVNVPLPVGEMTVKIVKVD
CCCCCHHHHHHCCCCCCEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471