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The map label for this gene is pepM

Identifier: 15672594

GI number: 15672594

Start: 600904

End: 601758

Strand: Direct

Name: pepM

Synonym: L883

Alternate gene names: 15672594

Gene position: 600904-601758 (Clockwise)

Preceding gene: 15672593

Following gene: 15672595

Centisome position: 25.4

GC content: 41.64

Gene sequence:

>855_bases
TTGATTACATTAAAATCGCAACGTGAAATCGAGCAAATGGAACGTAGTAGCAAAATTTTGGCAGATATCCACATTGGGCT
TCGTGAGTTGATTAAACCAGGGATTGACATGTGGGAAATTGAAGAATATGTCCGCAAAGTTTGTAAAGAAAAAAATGTTT
TACCTTTACAAATCGGAGTGGATGAAGGAAATTATAATCCATTCCCTTATGCTACTTGCTGCTGTTTGAATGATGAAGTG
GCTCACGCATTTCCACGTCATCAAATTCTTAAAGATGGTGATTTGATTAAGGTTGACATGGTTCTCGGGTTGGTTGAAGA
TGGTTCTGTTGATGTTTCAAAACTTAATTTTGACGATGCTGAATCAATGGTTAAATATCAAGAAGAATTCCGCGGTGGTG
TTGCTGACTCTTGCTGGGCTTATGCTGTAGGCCAAGTTTCTGACGAAGTAAAAAACTTGATGGATGTGACCCGCGAATGT
CTCTATCTTGGAATTGAACAAGCCAAAGTAGGCAACCGCATTGGTGATATTGGGGCAGCTATTCAAGAATACGCTGAAAG
TCGTGGCTATGGTGTCGTTCGTGACCTTGTAGGACATGGCGTTGGACCAACAATGCATGAAGAACCAATGGTGCCCCACT
ACGGACGTGCTGGACGTGGACTTCGTTTACGCGAAGGAATGGTCTTAACCATTGAACCAATGATTAACACTGGGGGTTGG
GAAATTGACCATGATGGTGAACGTGGTTACGTTACACTTGACGGAAGTTTATCATGTCAATACGAACACCAGTTTGTTAT
CACTAAAGATGGTCCTGTGATTTTGACTTCTCAAGGCGAAGAACGGACTTATTAA

Upstream 100 bases:

>100_bases
AATGAAAATTTATTTTTCGATGAAGTATGAGTCACTTCAAAACATTAATTTAAATAAAAAGAACTGAGTTTTTCTCAGAA
CTTTAGAAGGAAAGAATAAA

Downstream 100 bases:

>100_bases
GAAGTAAAAGGAAAAACAGATGAGAGGAAAGATGAAAAAAGTTATACAAAAAATTGCTGAACTAACGACAACTTTTATGA
CTTTTTTCAAAAGTTCAGAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MITLKSQREIEQMERSSKILADIHIGLRELIKPGIDMWEIEEYVRKVCKEKNVLPLQIGVDEGNYNPFPYATCCCLNDEV
AHAFPRHQILKDGDLIKVDMVLGLVEDGSVDVSKLNFDDAESMVKYQEEFRGGVADSCWAYAVGQVSDEVKNLMDVTREC
LYLGIEQAKVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPHYGRAGRGLRLREGMVLTIEPMINTGGW
EIDHDGERGYVTLDGSLSCQYEHQFVITKDGPVILTSQGEERTY

Sequences:

>Translated_284_residues
MITLKSQREIEQMERSSKILADIHIGLRELIKPGIDMWEIEEYVRKVCKEKNVLPLQIGVDEGNYNPFPYATCCCLNDEV
AHAFPRHQILKDGDLIKVDMVLGLVEDGSVDVSKLNFDDAESMVKYQEEFRGGVADSCWAYAVGQVSDEVKNLMDVTREC
LYLGIEQAKVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPHYGRAGRGLRLREGMVLTIEPMINTGGW
EIDHDGERGYVTLDGSLSCQYEHQFVITKDGPVILTSQGEERTY
>Mature_284_residues
MITLKSQREIEQMERSSKILADIHIGLRELIKPGIDMWEIEEYVRKVCKEKNVLPLQIGVDEGNYNPFPYATCCCLNDEV
AHAFPRHQILKDGDLIKVDMVLGLVEDGSVDVSKLNFDDAESMVKYQEEFRGGVADSCWAYAVGQVSDEVKNLMDVTREC
LYLGIEQAKVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPHYGRAGRGLRLREGMVLTIEPMINTGGW
EIDHDGERGYVTLDGSLSCQYEHQFVITKDGPVILTSQGEERTY

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=279, Percent_Identity=31.5412186379928, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI40385867, Length=281, Percent_Identity=26.3345195729537, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786364, Length=278, Percent_Identity=32.3741007194245, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71996291, Length=254, Percent_Identity=30.3149606299213, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323273, Length=253, Percent_Identity=31.2252964426877, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355531, Length=284, Percent_Identity=28.8732394366197, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24583427, Length=275, Percent_Identity=27.6363636363636, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 31800; Mature: 31800

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKSQREIEQMERSSKILADIHIGLRELIKPGIDMWEIEEYVRKVCKEKNVLPLQIGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEC
DEGNYNPFPYATCCCLNDEVAHAFPRHQILKDGDLIKVDMVLGLVEDGSVDVSKLNFDDA
CCCCCCCCCCEEEEECCCHHHHHCCHHHHCCCCCEEEEEHEEEEECCCCEEEEECCCCCH
ESMVKYQEEFRGGVADSCWAYAVGQVSDEVKNLMDVTRECLYLGIEQAKVGNRIGDIGAA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPHYGRAGRGLRLREGMVLTIEPMINTGGW
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCE
EIDHDGERGYVTLDGSLSCQYEHQFVITKDGPVILTSQGEERTY
EECCCCCCCEEEECCCEEEEEEEEEEEECCCCEEEECCCCCCCC
>Mature Secondary Structure
MITLKSQREIEQMERSSKILADIHIGLRELIKPGIDMWEIEEYVRKVCKEKNVLPLQIGV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEC
DEGNYNPFPYATCCCLNDEVAHAFPRHQILKDGDLIKVDMVLGLVEDGSVDVSKLNFDDA
CCCCCCCCCCEEEEECCCHHHHHCCHHHHCCCCCEEEEEHEEEEECCCCEEEEECCCCCH
ESMVKYQEEFRGGVADSCWAYAVGQVSDEVKNLMDVTRECLYLGIEQAKVGNRIGDIGAA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPHYGRAGRGLRLREGMVLTIEPMINTGGW
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCE
EIDHDGERGYVTLDGSLSCQYEHQFVITKDGPVILTSQGEERTY
EECCCCCCCEEEECCCEEEEEEEEEEEECCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192928; 11058132 [H]