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The map label for this gene is prfA

Identifier: 15672577

GI number: 15672577

Start: 585962

End: 587035

Strand: Direct

Name: prfA

Synonym: L0373

Alternate gene names: 15672577

Gene position: 585962-587035 (Clockwise)

Preceding gene: 15672576

Following gene: 15672578

Centisome position: 24.77

GC content: 36.59

Gene sequence:

>1074_bases
ATGTTTGATCAGTTAGAATCAATAGTTGGAAGATATGAAGAATTGGGTGAGTTATTGTCTGACCCTGAGGTGGTTTCGGA
TACAAAGCGTTTTATGGAATTATCGCGTGAAGAAGCAGATTTACGTGATAAAGTAGCGACTTATAATGAATACAAAAAAG
TTCTTGAAACAATTTCTGATAGTGAAGAAATGCTTGGCGAAGGTGGACTTGATGATGATATGAAAGAAATGCTCAAAGAA
GAACTTTCATCAGCAAAATCACAAAAAGAGCTTTTGGAAGAAGAAATCAAAATTTTGCTTTTGCCAAAAGACCCAAATGA
TGGAAAAAATATTATTTTAGAAATTCGTGGAGCTGCTGGTGGTGATGAAGCTGCACTTTTTGCTGGTGATTTGTTGAATA
TGTACCAACATTTTTCTGAATCACAAGGTTGGAAGTTTGAAATTATGGAAGCAAACATCACCGGAATTGGTGGATATAAA
GAGGTTTCAGCTTTAATTTCTGGTCCATCTGTTTATTCAAAATTGAAATATGAATCTGGTGCTCACCGAGTACAACGTGT
TCCTGTAACCGAAACACAAGGGCGGGTCCATACATCAACGGCTACTGTTCTTGTTATGCCTGAGGTTGAAGAATTTGAGA
TGACAATCGACCAAAAAGATTTACGTGTGGATATTTACCATGCCTCTGGTGCTGGTGGACAGAATGTCAATAAGGTTGCT
ACTGCGGTACGTATGGTCCATTTACCAACTGGGATTAAAGTGGAAATGCAAGAAGAACGGACGCAACAAAAAAATCGGGA
TAAAGCTATTAAATTGTTGAATACAAAAGTCTTTGATTACTATCAACAAATTGAATTGGATAAGCAAAATGCAGAACGTA
AATCAACTGTCGGAACTGGGGATCGTTCTGAGCGGATTAGAACTTACAATTTCCCACAAAACCGTGTGACTGATCACCGG
ATTGGCTTAACTTTACAAAAGTTGGATAGTATTTTATCTGGGAAAATGGATGAAGTGATTGATGCTTTGATTGTCTATGA
CCAAACTAAAAAATTAGAAGAATTAAATAAATAA

Upstream 100 bases:

>100_bases
AAAAAGACATGCTGCAAGATCTGACACAATGGGATTCGTTTGTCAATATGGCCAAAAATTTACCATTACAAACTAAAAAA
TAATTAAAGGAAATAGAGAA

Downstream 100 bases:

>100_bases
TGGATTTTGAATTTGAAGGTTTTGTTATTCGAGAAGTGGGACATGAAAATAGGAAAATGCAAACTTCAAAAAAGGTGAAT
AATGTTTCAACAGATGTGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDDMKEMLKE
ELSSAKSQKELLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIDQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNAERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK

Sequences:

>Translated_357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDDMKEMLKE
ELSSAKSQKELLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIDQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNAERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
>Mature_357_residues
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISDSEEMLGEGGLDDDMKEMLKE
ELSSAKSQKELLEEEIKILLLPKDPNDGKNIILEIRGAAGGDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYK
EVSALISGPSVYSKLKYESGAHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIDQKDLRVDIYHASGAGGQNVNKVA
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNAERKSTVGTGDRSERIRTYNFPQNRVTDHR
IGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=287, Percent_Identity=42.8571428571429, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI34577120, Length=351, Percent_Identity=37.8917378917379, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=43.1693989071038, Blast_Score=171, Evalue=7e-43,
Organism=Escherichia coli, GI1787462, Length=355, Percent_Identity=47.887323943662, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=34.5029239766082, Blast_Score=188, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=354, Percent_Identity=33.6158192090396, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=319, Percent_Identity=40.1253918495298, Blast_Score=229, Evalue=5e-61,
Organism=Drosophila melanogaster, GI19921226, Length=322, Percent_Identity=40.9937888198758, Blast_Score=224, Evalue=7e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_LACLA (Q9CHX3)

Other databases:

- EMBL:   AE005176
- PIR:   C86699
- RefSeq:   NP_266751.1
- ProteinModelPortal:   Q9CHX3
- SMR:   Q9CHX3
- GeneID:   1114215
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0373
- NMPDR:   fig|272623.1.peg.610
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   LLAC272623:L0373-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40405; Mature: 40405

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEEMLGEGGLDDDMKEMLKEELSSAKSQKELLEEEIKILLLPKDPNDGKNIILEIRGAAG
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCCC
GDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYKEVSALISGPSVYSKLKYESG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCC
AHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIDQKDLRVDIYHASGAGGQNVNKVA
CHHHEECCCCCCCCEEEECCEEEEEECCHHHHHHEECCCCCEEEEEECCCCCCCCHHHHH
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNAERKSTVGTG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
DRSERIRTYNFPQNRVTDHRIGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
CCCCCCEEECCCCCCCCHHHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFDQLESIVGRYEELGELLSDPEVVSDTKRFMELSREEADLRDKVATYNEYKKVLETISD
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SEEMLGEGGLDDDMKEMLKEELSSAKSQKELLEEEIKILLLPKDPNDGKNIILEIRGAAG
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCCC
GDEAALFAGDLLNMYQHFSESQGWKFEIMEANITGIGGYKEVSALISGPSVYSKLKYESG
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHCCC
AHRVQRVPVTETQGRVHTSTATVLVMPEVEEFEMTIDQKDLRVDIYHASGAGGQNVNKVA
CHHHEECCCCCCCCEEEECCEEEEEECCHHHHHHEECCCCCEEEEEECCCCCCCCHHHHH
TAVRMVHLPTGIKVEMQEERTQQKNRDKAIKLLNTKVFDYYQQIELDKQNAERKSTVGTG
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
DRSERIRTYNFPQNRVTDHRIGLTLQKLDSILSGKMDEVIDALIVYDQTKKLEELNK
CCCCCCEEECCCCCCCCHHHHCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471