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The map label for this gene is adaA

Identifier: 15672514

GI number: 15672514

Start: 519022

End: 519582

Strand: Direct

Name: adaA

Synonym: L0149

Alternate gene names: 15672514

Gene position: 519022-519582 (Clockwise)

Preceding gene: 15672513

Following gene: 15672515

Centisome position: 21.94

GC content: 34.05

Gene sequence:

>561_bases
TTGGAAATTACTGACGAAACTTGGGAAATCATTAAAAATAATGATAAAAACTTTGATAATAAGTTATGGTATGGCGTAGC
AACGACCAAAATATTTTGTCGTCCTTCCTGCGTCTCTAGACTACCAAAAAGAGAGAATGTAAGTATTTTTCAAGCAGCTG
AGCAAGCCTTAGAAGAAGGTTATCGTCCTTGCAAAAGATGTCGACCAATGGATAAAATTATACCAAATGAGGTTTGGGTA
GAAGAGATTGATTTACTTTTAAAAAATCATTATGATGAGGACTTAAGTCTTGAGGAGCTTGGACAAAGACTTCATGGTTC
TTCGTCTTATCTTCGCCATATTTATAAAAAAATAAAAGGGCTAACTCCACAGCAAGAACTAACAAGAATAAGACTTGAGC
AAGCAAGAATACGATTGCTTAAAGGAAATGAAGCAATCTCTGAAATTGCGCGTGACGTTGGAATGATGAATACTCCTTAT
TTTATAAAATCTTTTAAAAAGCGCTATGGACTTGCTCCCAATCAATACCGTAAAGCTTATAATATAAATTCCAAAAAATA
G

Upstream 100 bases:

>100_bases
ACCGATGGTTCCATGACAGGATACGCTGGTGGACTAGAGAAGAAAAAAGCTCTTTTGACACTTGAGCAAGCACATTTAAA
AAAATAATTGGAGTTTTCAT

Downstream 100 bases:

>100_bases
GAGAAAAAACTCTTATTTTTTTATCCCTAAAATTGCTTAATTTATCCCAGACTTTTGGTCAGTACTTTTGATAGATTGAG
TTAAAATAGCAATATGAAAA

Product: methylphosphotriester-DNA alkyltransferase

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MEITDETWEIIKNNDKNFDNKLWYGVATTKIFCRPSCVSRLPKRENVSIFQAAEQALEEGYRPCKRCRPMDKIIPNEVWV
EEIDLLLKNHYDEDLSLEELGQRLHGSSSYLRHIYKKIKGLTPQQELTRIRLEQARIRLLKGNEAISEIARDVGMMNTPY
FIKSFKKRYGLAPNQYRKAYNINSKK

Sequences:

>Translated_186_residues
MEITDETWEIIKNNDKNFDNKLWYGVATTKIFCRPSCVSRLPKRENVSIFQAAEQALEEGYRPCKRCRPMDKIIPNEVWV
EEIDLLLKNHYDEDLSLEELGQRLHGSSSYLRHIYKKIKGLTPQQELTRIRLEQARIRLLKGNEAISEIARDVGMMNTPY
FIKSFKKRYGLAPNQYRKAYNINSKK
>Mature_186_residues
MEITDETWEIIKNNDKNFDNKLWYGVATTKIFCRPSCVSRLPKRENVSIFQAAEQALEEGYRPCKRCRPMDKIIPNEVWV
EEIDLLLKNHYDEDLSLEELGQRLHGSSSYLRHIYKKIKGLTPQQELTRIRLEQARIRLLKGNEAISEIARDVGMMNTPY
FIKSFKKRYGLAPNQYRKAYNINSKK

Specific function: Methylphosphotriester-DNA alkyltransferase is one of two genes required for the adaptive response of bacteria to alkylating agents. It accepts a methyl group from methylphospho-triesters and then acts as a transcriptional activator of the ada operon [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=182, Percent_Identity=30.2197802197802, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR016220 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 21943; Mature: 21943

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITDETWEIIKNNDKNFDNKLWYGVATTKIFCRPSCVSRLPKRENVSIFQAAEQALEEG
CCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHEECCHHHHHCCCCCCHHHHHHHHHHHHHH
YRPCKRCRPMDKIIPNEVWVEEIDLLLKNHYDEDLSLEELGQRLHGSSSYLRHIYKKIKG
HHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
LTPQQELTRIRLEQARIRLLKGNEAISEIARDVGMMNTPYFIKSFKKRYGLAPNQYRKAY
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
NINSKK
CCCCCC
>Mature Secondary Structure
MEITDETWEIIKNNDKNFDNKLWYGVATTKIFCRPSCVSRLPKRENVSIFQAAEQALEEG
CCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHEECCHHHHHCCCCCCHHHHHHHHHHHHHH
YRPCKRCRPMDKIIPNEVWVEEIDLLLKNHYDEDLSLEELGQRLHGSSSYLRHIYKKIKG
HHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHC
LTPQQELTRIRLEQARIRLLKGNEAISEIARDVGMMNTPYFIKSFKKRYGLAPNQYRKAY
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHH
NINSKK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]