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The map label for this gene is ppnK

Identifier: 15672345

GI number: 15672345

Start: 366739

End: 367551

Strand: Direct

Name: ppnK

Synonym: L166614

Alternate gene names: 15672345

Gene position: 366739-367551 (Clockwise)

Preceding gene: 30023987

Following gene: 15672346

Centisome position: 15.5

GC content: 37.88

Gene sequence:

>813_bases
ATGAACTTTGGTAAAAAAGTCTGGCTTATTGGAAATTCGAGTGAAAAATCCAAAAAAACGCTCAATAAATTGAGCAAGAT
TTTGAAAGCTGAACATTTCGTTTTTGATGATATTAATCCTGAAATTGTGATTTCAGTTGGAGGAGATGGGACGCTTTTAA
GAGCAATGCACATGTACGAATATCAGCTAGATCGTGTGCGATTTTTGGGTGTTCATACGGGGCATTTGGGCTTTTATACT
GATTTTACTGACGAAGATTTGTTTGAAGTCGTAGAGGCTCTTTATGATGAAAATCCAGCTCAAGCGATTCACTATCCACT
CATTCGTGTTCAAGTTAGTTTTACTGACGGATATCAAATTGTACGACATGTTCTCAATGAAGCAACGATTCGACGTGCAA
GTAAAACGATGGTTGGGGATGTTAGAATTTCTGATTATCTTTTTGAACGTTTTCGTGGGGACGGCTTGTCAATTTCAACT
CCAACAGGTTCAACGGCTTATAATAAATCAATTGGAGGAGCCGTTGTTCATCCGCGGGTAAAAGCAATGCAAGTTGCTGA
AATTGCAAGTTTGAACAATGTCGTTTATCGAACATTAGGTTCACCAATGATTGTAGCTGAGAAAGATACAATTACCGTTT
GTCCTGCGCCAGAGGATGATTATAGTTTGACTTTTGATCAGTTGACTTTTGAATACAAAAATATCAAATCAATTGAGTTT
TCTTTGGATGGAACAACGATTTCTTTTGCAAATTGTGCTCACACACCATTTTGGGAACGGGTCAGTAAGTCCTTTATTGG
GGAGGTTGAGTAA

Upstream 100 bases:

>100_bases
TTGGAAATGGGCGAAATCCGTGAGGATATTCAAGAAGCACAGTTACTTTTTGATAGTCCTCAGCAAAAACAAGACTTTAA
AAAAGAAAATGGTGAAGAAA

Downstream 100 bases:

>100_bases
TGCAGTTTGCTTTTAGAAACGAGATTGAAGGAAGCATGGTTAAATCAATGCTAAGCCGTCACGGTATTTCTAGGAGATTG
CTGACAAAAGTAAAATTTGA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLIRVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQVAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE

Sequences:

>Translated_270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLIRVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQVAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE
>Mature_270_residues
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYEYQLDRVRFLGVHTGHLGFYT
DFTDEDLFEVVEALYDENPAQAIHYPLIRVQVSFTDGYQIVRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSIST
PTGSTAYNKSIGGAVVHPRVKAMQVAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
SLDGTTISFANCAHTPFWERVSKSFIGEVE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=157, Percent_Identity=29.2993630573248, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320794, Length=192, Percent_Identity=31.7708333333333, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325068, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322509, Length=175, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_LACLA (Q9CIJ4)

Other databases:

- EMBL:   AE005176
- PIR:   C86670
- RefSeq:   NP_266519.1
- ProteinModelPortal:   Q9CIJ4
- SMR:   Q9CIJ4
- GeneID:   1113974
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L166614
- NMPDR:   fig|272623.1.peg.371
- HOGENOM:   HBG314020
- OMA:   TISFANC
- ProtClustDB:   PRK04885
- BioCyc:   LLAC272623:L166614-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30382; Mature: 30382

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYE
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHH
YQLDRVRFLGVHTGHLGFYTDFTDEDLFEVVEALYDENPAQAIHYPLIRVQVSFTDGYQI
HHHHHEEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCEEECEEEEEEEEECCCHHH
VRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSISTPTGSTAYNKSIGGAVVHPRV
HHHHHHHHHHHHHHHHEECCEEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCH
KAMQVAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEHHHEEEEECCCEEEEE
SLDGTTISFANCAHTPFWERVSKSFIGEVE
EECCCEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNFGKKVWLIGNSSEKSKKTLNKLSKILKAEHFVFDDINPEIVISVGGDGTLLRAMHMYE
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCHHHHHHHHHH
YQLDRVRFLGVHTGHLGFYTDFTDEDLFEVVEALYDENPAQAIHYPLIRVQVSFTDGYQI
HHHHHEEEEEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCEEECEEEEEEEEECCCHHH
VRHVLNEATIRRASKTMVGDVRISDYLFERFRGDGLSISTPTGSTAYNKSIGGAVVHPRV
HHHHHHHHHHHHHHHHEECCEEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCH
KAMQVAEIASLNNVVYRTLGSPMIVAEKDTITVCPAPEDDYSLTFDQLTFEYKNIKSIEF
HHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCEEEHHHEEEEECCCEEEEE
SLDGTTISFANCAHTPFWERVSKSFIGEVE
EECCCEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471