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The map label for this gene is eno

Identifier: 15672258

GI number: 15672258

Start: 274176

End: 275444

Strand: Direct

Name: eno

Synonym: L0008

Alternate gene names: 15672258

Gene position: 274176-275444 (Clockwise)

Preceding gene: 15672256

Following gene: 15672259

Centisome position: 11.59

GC content: 37.98

Gene sequence:

>1269_bases
ATGACAGTCACAATCGAAAACATCCACGCAAGAGAAATTTTTGATTCTAGAGGAAATCCAACCGTTGAGGTAGATGTTAG
ATTAACAGATGGAACATTAGGACGCGCAGCAGTACCCTCAGGAGCATCAACTGGAGACCGTGAAGCAGTTGAATTAAGAG
ATGGTGGAGACCGTCTTCAAGGGAAAGGCGTTTCTAAAGCCGTCGCAAATGTCAATGGCGAAATTTATGAAGCCTTGAAA
GGACAATCACCATTTAATCAAGCAAAATTAGATCATTTGATGATTGAGCTTGACGGGACAAAAAATAAATCTCGTTTAGG
GGCTAATGCAATTTTAGGGGTTTCAATGGCCATTTCTCGTGCGGCAGCGAATAGTGAAAAAATTCCACTCTATCGCTATC
TTGGTGGAGTTGATTTGGAACTTCCACAACCATTTTTCAATGTCATTAATGGTGGAGTACATGCCGATTCAGGAATTGAC
GTTCAAGAATTTTTGATTACACCAGTCAAACGGGAAAGTTTTCGCGATGGTTTAGAAAAAATTGCAAATATCTACCATAC
TCTGAAGAAAATTTTAGCTGACAAAGGGCTAGAAACAGCCGTAGGTGATGAAGGTGGCTTTGCCCCTAAACTTGGTTCTA
CTGAAAATGCAATTGCTACACTTTATCAAGCAATTGAAAGTGCAGGATATGTTCCTGGTGAAGAAATTGCGATTGCCATT
GACCCAGCTTCAAGTGAATTTTATGATGATAAAGAAAAAGTTTATCGTTTTGAAGGGCAAAAATTGACTTCTAAAGAACT
ATTGACTTACTATGAAAATTTAGTTGAAAAATATCCAGCACTTATTTCTATTGAAGATGGATTTTCTGAACATGACTGGG
AGGGCTTTGCAGCTCAAACGAAAGCTCAAGGTCAAAAAATTCAATTAGTAGGTGATGATATTTTTGTGACAAATCCTGAA
ATTTTCAAAGAAGGAATTAAAAAAGGTGTAGCAAATGCTATTTTGATTAAACTTAATCAAATCGGAACAGTAACTGAAGC
GATTGAAGCCATTAGTTTAGCGAGAAAAGCAGGCTATAAGACAATGATTTCTCATCGTTCAGGTGAAACAGTTGATAGCT
ACATTGCCGATTTTGCGGTAGCCATGCATGCTGGCCAAATAAAAACAGGTTCAATGGCTCGAAGTGAACGGGTTGAAAAA
TATAATCAATTCTTGCGAATTGAAGAAGAATTAGGAAAAGATGTAGCTCTTGCTAGTTTTCCAGGATAG

Upstream 100 bases:

>100_bases
ATCTAATGGCGCCTACAAAGCAAAGTCTTTTTTTGCCTTGTAGTCTGCCATTTTTTCTTTGACTTACAAGGCATTAAAGG
CAAAGAAAAGGAGATTTATT

Downstream 100 bases:

>100_bases
ATAAAAATAAAAAAACATTAATTTTTATAATTAATGTTTTTTTATTTTTAAGAATGTGAATTTATAAAGGAGAAGAGGGG
ATTCGAACCCCCGATACGCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTVTIENIHAREIFDSRGNPTVEVDVRLTDGTLGRAAVPSGASTGDREAVELRDGGDRLQGKGVSKAVANVNGEIYEALK
GQSPFNQAKLDHLMIELDGTKNKSRLGANAILGVSMAISRAAANSEKIPLYRYLGGVDLELPQPFFNVINGGVHADSGID
VQEFLITPVKRESFRDGLEKIANIYHTLKKILADKGLETAVGDEGGFAPKLGSTENAIATLYQAIESAGYVPGEEIAIAI
DPASSEFYDDKEKVYRFEGQKLTSKELLTYYENLVEKYPALISIEDGFSEHDWEGFAAQTKAQGQKIQLVGDDIFVTNPE
IFKEGIKKGVANAILIKLNQIGTVTEAIEAISLARKAGYKTMISHRSGETVDSYIADFAVAMHAGQIKTGSMARSERVEK
YNQFLRIEEELGKDVALASFPG

Sequences:

>Translated_422_residues
MTVTIENIHAREIFDSRGNPTVEVDVRLTDGTLGRAAVPSGASTGDREAVELRDGGDRLQGKGVSKAVANVNGEIYEALK
GQSPFNQAKLDHLMIELDGTKNKSRLGANAILGVSMAISRAAANSEKIPLYRYLGGVDLELPQPFFNVINGGVHADSGID
VQEFLITPVKRESFRDGLEKIANIYHTLKKILADKGLETAVGDEGGFAPKLGSTENAIATLYQAIESAGYVPGEEIAIAI
DPASSEFYDDKEKVYRFEGQKLTSKELLTYYENLVEKYPALISIEDGFSEHDWEGFAAQTKAQGQKIQLVGDDIFVTNPE
IFKEGIKKGVANAILIKLNQIGTVTEAIEAISLARKAGYKTMISHRSGETVDSYIADFAVAMHAGQIKTGSMARSERVEK
YNQFLRIEEELGKDVALASFPG
>Mature_421_residues
TVTIENIHAREIFDSRGNPTVEVDVRLTDGTLGRAAVPSGASTGDREAVELRDGGDRLQGKGVSKAVANVNGEIYEALKG
QSPFNQAKLDHLMIELDGTKNKSRLGANAILGVSMAISRAAANSEKIPLYRYLGGVDLELPQPFFNVINGGVHADSGIDV
QEFLITPVKRESFRDGLEKIANIYHTLKKILADKGLETAVGDEGGFAPKLGSTENAIATLYQAIESAGYVPGEEIAIAID
PASSEFYDDKEKVYRFEGQKLTSKELLTYYENLVEKYPALISIEDGFSEHDWEGFAAQTKAQGQKIQLVGDDIFVTNPEI
FKEGIKKGVANAILIKLNQIGTVTEAIEAISLARKAGYKTMISHRSGETVDSYIADFAVAMHAGQIKTGSMARSERVEKY
NQFLRIEEELGKDVALASFPG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=51.1627906976744, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=47.906976744186, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=47.906976744186, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=47.6744186046512, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=42.6229508196721, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=416, Percent_Identity=55.2884615384615, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=48.2598607888631, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=48.2598607888631, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32563855, Length=269, Percent_Identity=39.7769516728625, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=46.6512702078522, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=46.6512702078522, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=46.6512702078522, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=45.0346420323326, Blast_Score=332, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6321968, Length=427, Percent_Identity=44.4964871194379, Blast_Score=309, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=47.7958236658933, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=47.7958236658933, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=47.7958236658933, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=47.7958236658933, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=47.7958236658933, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=47.7958236658933, Blast_Score=352, Evalue=3e-97,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO2_LACLA (Q9CIT0)

Other databases:

- EMBL:   AE005176
- PIR:   D86659
- RefSeq:   NP_266432.1
- ProteinModelPortal:   Q9CIT0
- SMR:   Q9CIT0
- GeneID:   1113886
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0008
- NMPDR:   fig|272623.1.peg.284
- HOGENOM:   HBG726599
- OMA:   NVNTEIN
- ProtClustDB:   PRK00077
- BioCyc:   LLAC272623:L0008-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45809; Mature: 45678

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 337-337 BINDING 154-154 BINDING 163-163 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTIENIHAREIFDSRGNPTVEVDVRLTDGTLGRAAVPSGASTGDREAVELRDGGDRLQ
CEEEEECCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GKGVSKAVANVNGEIYEALKGQSPFNQAKLDHLMIELDGTKNKSRLGANAILGVSMAISR
CCCHHHHHHHCCHHHHHHHCCCCCCCHHHHEEEEEEECCCCCCHHCCCHHHHHHHHHHHH
AAANSEKIPLYRYLGGVDLELPQPFFNVINGGVHADSGIDVQEFLITPVKRESFRDGLEK
HHCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
IANIYHTLKKILADKGLETAVGDEGGFAPKLGSTENAIATLYQAIESAGYVPGEEIAIAI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DPASSEFYDDKEKVYRFEGQKLTSKELLTYYENLVEKYPALISIEDGFSEHDWEGFAAQT
CCCCCCCCCCHHHHHECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
KAQGQKIQLVGDDIFVTNPEIFKEGIKKGVANAILIKLNQIGTVTEAIEAISLARKAGYK
HCCCCEEEEECCCEEECCHHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHHHHHHCHH
TMISHRSGETVDSYIADFAVAMHAGQIKTGSMARSERVEKYNQFLRIEEELGKDVALASF
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
PG
CC
>Mature Secondary Structure 
TVTIENIHAREIFDSRGNPTVEVDVRLTDGTLGRAAVPSGASTGDREAVELRDGGDRLQ
EEEEECCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GKGVSKAVANVNGEIYEALKGQSPFNQAKLDHLMIELDGTKNKSRLGANAILGVSMAISR
CCCHHHHHHHCCHHHHHHHCCCCCCCHHHHEEEEEEECCCCCCHHCCCHHHHHHHHHHHH
AAANSEKIPLYRYLGGVDLELPQPFFNVINGGVHADSGIDVQEFLITPVKRESFRDGLEK
HHCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
IANIYHTLKKILADKGLETAVGDEGGFAPKLGSTENAIATLYQAIESAGYVPGEEIAIAI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
DPASSEFYDDKEKVYRFEGQKLTSKELLTYYENLVEKYPALISIEDGFSEHDWEGFAAQT
CCCCCCCCCCHHHHHECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
KAQGQKIQLVGDDIFVTNPEIFKEGIKKGVANAILIKLNQIGTVTEAIEAISLARKAGYK
HCCCCEEEEECCCEEECCHHHHHHHHHHHCCCEEEEEEHHCCHHHHHHHHHHHHHHHCHH
TMISHRSGETVDSYIADFAVAMHAGQIKTGSMARSERVEKYNQFLRIEEELGKDVALASF
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCC
PG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471