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The map label for this gene is pmrB

Identifier: 15672108

GI number: 15672108

Start: 129376

End: 130572

Strand: Reverse

Name: pmrB

Synonym: L129379

Alternate gene names: 15672108

Gene position: 130572-129376 (Counterclockwise)

Preceding gene: 15672122

Following gene: 15672103

Centisome position: 5.52

GC content: 39.35

Gene sequence:

>1197_bases
ATGAAAGAATCATGGCAACGAAATTTAATTGTCCTTTGGTTTGGGACATTTATGACGGGGATAGGTTCAAGTCTTATCGC
CCCTTTTATATCACTTTACATCAGTACACTTGGCCATTACTCAAAAACGGAATTAAACATCTGGTCAGGCTTGGTCTTCT
CATCCACCTTTATTGTTTTAGCAATCGTTTCTCCCCTTTGGGGAAGATTAGCTGACCAGAAAGGTCGGAAATTGATGCTC
TTACGCGCTTCTCTTGGGATGGCAATTTCAATTTCATTAATGGCTTTTGTTACAGCTGCTTGGCAATTGCTTATTTTACG
CATGCTACTTGGGGCCTTTTCTGGTTTTGTCTCTAATTCAATGGCCTTAATGGCCAGTTCTGCACCAAAGGAAAAATCAG
GGGCCGTCTTATCGCTCCTTACAACAGGATCTGTTGCTGGAACTCTTATCGGTCCAATCATTGGCGGAATACTTGTTAAT
ATAACTGGTTATCGTCGCGTTTTTAGCGTCACAGGAATTATTATGTTTCTCGTCTTTTTCCTTGCTTTATTTTTTGTCAA
AGAAATATTTAAGCCCGTTGAGAAGAAAAACATGCTTTCCTATAAACAAGTTTGGGAAGTTGTTACCCACCCAGCCATTA
TTTGGGGCATGTTTTTTGCAACCCTCATTACTCAAATGACCAATCAATCGATTAATCCAGTTCTTTCACTCTATGTCCAA
GAACTTATGCATGGTCAAGGAAACATTACTTTCATGGCCGGAATTGTCGCTGCTGCCCCAGGAATCGTTACATTAGTGGC
TGCTCCATTCTTAGGTCGTCTAGGTGATAAAGTTGGTCAAAGAAAAATCCTTGGTTTTGGACTTTTCTTTTCACTAATCG
TCTTCTCAATCACAGCAATGACAACTAATGTTTGGTTTTTAATTGTTATGAGACTACTTGTCGGAGTCTCTGATGCGGCA
ATTCTCCCTTCAGTTCAAGCCATCTTAGCAAAAGAATCTCCTCAAGCTATTACTGGTCGAATTTTCTCTTATAATCAATC
TGCTCAATCAATTGGTGCTTTTGCAGGTCCCTTACTTGGTTCAGCTATTGCTGGCTTTATTGACTATCGCTATGTCTTTG
TGGGTTCAGCAATTCTGGTTGTCTTTAATTTAATAAACTACTTTACACATACTAAATCACTTGCTGTAAAAAACTAA

Upstream 100 bases:

>100_bases
ATTCTCAATCTTTTTCAAGCAATGATTCGTCATTGACAAACTACGAATAATAGTTTTATAATATTATTATGAATACATCA
AGAAAGAAGGGCATTCATTC

Downstream 100 bases:

>100_bases
TTATAAAAAAGAACTTCAAATTTGAAGTTCTTTTTTAGCTTCTTCTATTTGTTTTTTAACCGATTCAAATCCTGTACCAC
CTAGAGAATTTCTTCTTGAA

Product: multidrug resistance efflux pump

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLML
LRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVN
ITGYRRVFSVTGIIMFLVFFLALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQ
ELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAMTTNVWFLIVMRLLVGVSDAA
ILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVVFNLINYFTHTKSLAVKN

Sequences:

>Translated_398_residues
MKESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLML
LRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVN
ITGYRRVFSVTGIIMFLVFFLALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQ
ELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAMTTNVWFLIVMRLLVGVSDAA
ILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVVFNLINYFTHTKSLAVKN
>Mature_398_residues
MKESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVLAIVSPLWGRLADQKGRKLML
LRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNSMALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVN
ITGYRRVFSVTGIIMFLVFFLALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQ
ELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAMTTNVWFLIVMRLLVGVSDAA
ILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAGPLLGSAIAGFIDYRYVFVGSAILVVFNLINYFTHTKSLAVKN

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=382, Percent_Identity=46.3350785340314, Blast_Score=330, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146993, Length=378, Percent_Identity=24.3386243386243, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43230; Mature: 43230

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVL
CCCCHHCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNS
HHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIMFLVFF
HHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAM
HHHCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTNVWFLIVMRLLVGVSDAAILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAGPLLG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
SAIAGFIDYRYVFVGSAILVVFNLINYFTHTKSLAVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKESWQRNLIVLWFGTFMTGIGSSLIAPFISLYISTLGHYSKTELNIWSGLVFSSTFIVL
CCCCHHCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
AIVSPLWGRLADQKGRKLMLLRASLGMAISISLMAFVTAAWQLLILRMLLGAFSGFVSNS
HHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MALMASSAPKEKSGAVLSLLTTGSVAGTLIGPIIGGILVNITGYRRVFSVTGIIMFLVFF
HHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALFFVKEIFKPVEKKNMLSYKQVWEVVTHPAIIWGMFFATLITQMTNQSINPVLSLYVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ELMHGQGNITFMAGIVAAAPGIVTLVAAPFLGRLGDKVGQRKILGFGLFFSLIVFSITAM
HHHCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTNVWFLIVMRLLVGVSDAAILPSVQAILAKESPQAITGRIFSYNQSAQSIGAFAGPLLG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
SAIAGFIDYRYVFVGSAILVVFNLINYFTHTKSLAVKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA