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The map label for this gene is mptN

Identifier: 15668801

GI number: 15668801

Start: 549689

End: 550564

Strand: Direct

Name: mptN

Synonym: MJ0620

Alternate gene names: 15668801

Gene position: 549689-550564 (Clockwise)

Preceding gene: 15668798

Following gene: 15668804

Centisome position: 33.01

GC content: 29.22

Gene sequence:

>876_bases
ATGAAACTTGGTATAATTACCATAGAAAGAGATGCTGTAGTCAATGATTTAATAAAATCCTGTGAAAAGTATGAAGTAGA
TTATAAAGTTATAACCCCTTCAAATATTGTAGCTGGATTTAATTTGGATTTTAAATTAAAATATTACAAGTCATTTTTAG
ATGAATTAGATTGCTGTTTTGTTAGAAATCTTGGCTGGGATAGTTTTTTTAGATTTGATGTCTTAAAGTATTTAAATCAT
TATATTCCCGTTATAAATCCTCCAGATGGAATAGATAGGGCATCAAATAAGTTTTTAACCTCTGTATTTCTTGAATTAAA
TAATCTCCCACAACCAAAGACTGTTGTTACTGAAAGTATAAATGAAGCAATTGTCTGGATAGATAAATTTGAAGAAGCAG
TTTTAAAACCAATATTTGGATGTGGTGGGGAGGGAATTGTTAGGGTTAAAAAAGAACTGCCAATATCTACTAAGTTAAAG
ATTTTAAATGAATTTAAAGAAAAATATAATACCTTCTATATCCAGGAATTTATAAAACCAGTAAGAAATGAACATAGAGA
TATAAGGGCTTTTGTAGTTGATGATGAGGTCGTTGCGGCAATGTATAGGATTGGAGGAGAAAATTGGAAAAACAATGTTT
CTCAGGGAGGAAGAGTTGAGAAATGTGAAATAACTGAAGAAATTGAAAAATTGGCTTTAAAAGCAAAAAATGCTCTTGGT
TTATTTTATGCTGGAGTTGATTTAATTGAGTCAGAAGATGGTTTAAAAGTTTTAGAAGTAAATTCAACACCATCTTGGAT
TGGATTATCTAAGGTCTCTGAGGTTAATATAGCCGATAAACTCTTAGAAAAAATAATTCAATATGTTAAATCTTAA

Upstream 100 bases:

>100_bases
TTTACTCTATGCAATAAAGATAAAACTACATAACATACTTATAATTAATTGTTATATACTTCATTAATATTTAACTGTTT
AGTTATATAAGGGGATAGTA

Downstream 100 bases:

>100_bases
TATATTATTCATTTATTTTCTTTGCAATTATTTCTAATGCTTTCATAAACGCATCCTTTTCTGGAATAATAACAGCTACA
GGGACATTAACGATTTTTTC

Product: ribosomal protein S6 modification protein 1 RimK

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCFVRNLGWDSFFRFDVLKYLNH
YIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESINEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLK
ILNEFKEKYNTFYIQEFIKPVRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALG
LFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS

Sequences:

>Translated_291_residues
MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCFVRNLGWDSFFRFDVLKYLNH
YIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESINEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLK
ILNEFKEKYNTFYIQEFIKPVRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALG
LFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS
>Mature_291_residues
MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCFVRNLGWDSFFRFDVLKYLNH
YIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESINEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLK
ILNEFKEKYNTFYIQEFIKPVRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALG
LFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1787076, Length=210, Percent_Identity=29.0476190476191, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789316, Length=188, Percent_Identity=30.8510638297872, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MPTN_METJA (Q58037)

Other databases:

- EMBL:   L77117
- PIR:   D64377
- RefSeq:   NP_247604.1
- ProteinModelPortal:   Q58037
- GeneID:   1451486
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0620
- NMPDR:   fig|243232.1.peg.641
- TIGR:   MJ0620
- HOGENOM:   HBG679591
- OMA:   ITNIHQN
- ProtClustDB:   CLSK876269
- BioCyc:   MetaCyc:MONOMER-15293
- BioCyc:   MJAN243232:MJ_0620-MONOMER
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 33278; Mature: 33278

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCF
CCEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEHHHHHHHHHHHHHHH
VRNLGWDSFFRFDVLKYLNHYIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESI
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLKILNEFKEKYNTFYIQEFIKP
HHHEEHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALG
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHCHHE
LFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS
EHHHCCHHEECCCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCF
CCEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCEEHHHHHHHHHHHHHHH
VRNLGWDSFFRFDVLKYLNHYIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESI
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
NEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLKILNEFKEKYNTFYIQEFIKP
HHHEEHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALG
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHCHHE
LFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS
EHHHCCHHEECCCCCEEEEECCCCCCCCHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087