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The map label for this gene is thrA [C]

Identifier: 15668751

GI number: 15668751

Start: 508398

End: 509819

Strand: Direct

Name: thrA [C]

Synonym: MJ0571

Alternate gene names: 15668751

Gene position: 508398-509819 (Clockwise)

Preceding gene: 15668748

Following gene: 15668752

Centisome position: 30.53

GC content: 35.3

Gene sequence:

>1422_bases
ATGACAACAGTAATGAAGTTTGGAGGAACTTCTGTAGGTTCTGGAGAAAGAATTAGGCATGTGGCGAAAATAGTAACAAA
GAGAAAAAAAGAGGATGATGATGTGGTTGTTGTAGTTTCAGCAATGAGTGAAGTAACTAACGCATTGGTGGAGATATCTC
AGCAAGCTTTAGATGTTAGAGATATCGCAAAAGTAGGAGATTTTATAAAATTTATTAGAGAGAAACACTACAAAGCTATA
GAAGAAGCTATAAAATCAGAAGAAATTAAAGAAGAAGTAAAAAAAATAATTGACAGCAGGATTGAAGAATTAGAGAAGGT
TTTAATTGGTGTAGCATACTTAGGGGAGCTTACACCAAAGTCAAGAGACTATATATTATCATTTGGAGAGAGGTTGTCCT
CACCAATATTAAGTGGAGCTATTAGAGATTTAGGAGAAAAGTCTATTGCTTTAGAAGGAGGAGAAGCAGGAATAATAACG
GATAACAACTTTGGAAGTGCAAGAGTTAAAAGATTAGAGGTTAAAGAGAGATTGTTACCATTATTAAAAGAGGGCATTAT
TCCAGTGGTTACAGGATTTATAGGAACCACTGAAGAGGGTTATATAACAACCTTAGGAAGAGGCGGAAGTGATTACTCAG
CCGCTTTAATTGGTTATGGCTTAGATGCAGATATTATTGAAATTTGGACAGATGTTTCTGGAGTTTATACAACAGACCCA
AGATTAGTTCCTACTGCAAGAAGAATTCCAAAACTTAGTTACATAGAGGCTATGGAATTAGCATACTTTGGAGCTAAGGT
TTTGCATCCAAGAACTATAGAGCCAGCTATGGAGAAGGGCATTCCAATATTGGTAAAGAATACATTTGAGCCAGAGAGTG
AAGGAACTTTAATAACCAACGATATGGAGATGAGTGATAGCATAGTTAAAGCTATATCAACAATAAAAAATGTCGCTTTA
ATAAACATATTTGGAGCTGGGATGGTTGGAGTTAGTGGAACAGCGGCAAGGATATTCAAAGCTTTAGGAGAAGAGGAAGT
TAATGTAATTTTAATAAGCCAAGGTTCCTCTGAAACAAATATATCCCTCGTTGTAAGTGAGGAGGATGTTGATAAAGCAT
TAAAAGCATTAAAGAGAGAGTTTGGGGATTTTGGAAAGAAGAGCTTTTTAAACAATAACTTAATTAGGGATGTGAGTGTT
GATAAAGATGTTTGTGTTATTTCAGTTGTAGGGGCTGGAATGAGAGGAGCTAAAGGCATAGCTGGAAAGATATTCACTGC
TGTTTCTGAAAGCGGGGCAAATATAAAGATGATAGCTCAAGGTTCATCAGAAGTGAATATATCATTTGTTATTGATGAAA
AGGATTTGTTGAATTGTGTTAGAAAATTGCACGAGAAGTTTATTGAAAAAACTAACAGCTAA

Upstream 100 bases:

>100_bases
TAACTCCTAACTCCCCAATAAAGAAAAATTTTAAATATACATGGTCTAAAATTTTGTGTGGTTAATCTTATCAATTATAA
AATAATTTTTGGTGTAAGTT

Downstream 100 bases:

>100_bases
AATCTTTTATTTTTTATTTTTAATTTTTAGGGTGTTAATATGGATGAAAATATTAAAAAGGCAGAGTATTATTATAAAAA
AGGTGTAGAAGTTGGAAATA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAI
EEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIIT
DNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDP
RLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDMEMSDSIVKAISTIKNVAL
INIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSV
DKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS

Sequences:

>Translated_473_residues
MTTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAI
EEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIIT
DNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDP
RLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDMEMSDSIVKAISTIKNVAL
INIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSV
DKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS
>Mature_472_residues
TTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAIE
EAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITD
NNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPR
LVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDMEMSDSIVKAISTIKNVALI
NIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSVD
KDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=471, Percent_Identity=36.3057324840764, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1790455, Length=473, Percent_Identity=30.6553911205074, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790376, Length=384, Percent_Identity=27.6041666666667, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320893, Length=506, Percent_Identity=32.2134387351779, Blast_Score=227, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK_METJA (Q57991)

Other databases:

- EMBL:   L77117
- PIR:   C64371
- RefSeq:   NP_247550.1
- PDB:   2HMF
- PDB:   3C1M
- PDB:   3C1N
- PDB:   3C20
- PDBsum:   2HMF
- PDBsum:   3C1M
- PDBsum:   3C1N
- PDBsum:   3C20
- ProteinModelPortal:   Q57991
- SMR:   Q57991
- GeneID:   1451436
- GenomeReviews:   L77117_GR
- KEGG:   mja:MJ_0571
- NMPDR:   fig|243232.1.peg.587
- TIGR:   MJ0571
- HOGENOM:   HBG724395
- OMA:   GSSETNI
- ProtClustDB:   PRK06291
- BioCyc:   MJAN243232:MJ_0571-MONOMER
- BRENDA:   2.7.2.4
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00656
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 51393; Mature: 51262

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVR
CCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
DIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLP
CHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
LLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDP
HHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHEEECCCCHHHHHHHHCCCCEEECCC
RLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITN
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEC
DMEMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETN
CHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
ISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGI
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCCCC
AGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS
HHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVR
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
DIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLP
CHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
LLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDP
HHHHCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHEEECCCCHHHHHHHHCCCCEEECCC
RLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITN
CCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEC
DMEMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETN
CHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCC
ISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGI
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCCCC
AGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS
HHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087