Click here to switch to the map view.

The map label for this gene is aroK

Identifier: 15607112

GI number: 15607112

Start: 1532112

End: 1532618

Strand: Direct

Name: aroK

Synonym: aq_2177

Alternate gene names: 15607112

Gene position: 1532112-1532618 (Clockwise)

Preceding gene: 15607111

Following gene: 15607114

Centisome position: 98.76

GC content: 42.6

Gene sequence:

>507_bases
ATGAGGATTTACCTTATAGGCTTTATGTGCTCCGGGAAAAGCACAGTTGGTTCCCTCCTCTCCCGCTCTTTAAACATTCC
CTTCTATGACGTTGACGAAGAGGTGCAGAAAAGGGAAGGACTTTCAATCCCCCAAATCTTTGAAAAGAAAGGGGAAGCTT
ACTTCAGAAAGCTTGAGTTTGAAGTCTTAAAGGATTTATCCGAAAAAGAAAACGTAGTCATTTCCACGGGAGGAGGACTC
GGGGCAAACGAAGAAGCATTAAACTTTATGAAGAGTAGAGGCACAACCGTTTTCATAGATATCCCCTTTGAGGTTTTCCT
TGAAAGGTGTAAGGACTCAAAGGAGAGACCCCTTTTAAAGAGACCTCTCGATGAGATAAAAAACTTATTTGAAGAGAGGA
GAAAGATTTATTCAAAGGCGGACATAAAGGTGAAGGGGGAAAAACCCCCCGAAGAGGTTGTAAAGGAAATTTTATTATCC
CTTGAAGGGAACGCCCTTGGTGGGTGA

Upstream 100 bases:

>100_bases
CAAAACACCAGAGGATAGCAAATAAGTACGGACTTTCGTGTAAGGACTGCCACTACGGAATTACAGAGTACAGAGGATAC
GAGGAGGGAACAACTGAACA

Downstream 100 bases:

>100_bases
AGAGGGAACTGCGTAAGTTCTCTTCGGCATCCTTCCGGCAAGGTAAGCAAGTCTTCCGGCGATTGTTGCGTACTTCATAG
CCACGGCCATCTTTATAGGA

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MRIYLIGFMCSGKSTVGSLLSRSLNIPFYDVDEEVQKREGLSIPQIFEKKGEAYFRKLEFEVLKDLSEKENVVISTGGGL
GANEEALNFMKSRGTTVFIDIPFEVFLERCKDSKERPLLKRPLDEIKNLFEERRKIYSKADIKVKGEKPPEEVVKEILLS
LEGNALGG

Sequences:

>Translated_168_residues
MRIYLIGFMCSGKSTVGSLLSRSLNIPFYDVDEEVQKREGLSIPQIFEKKGEAYFRKLEFEVLKDLSEKENVVISTGGGL
GANEEALNFMKSRGTTVFIDIPFEVFLERCKDSKERPLLKRPLDEIKNLFEERRKIYSKADIKVKGEKPPEEVVKEILLS
LEGNALGG
>Mature_168_residues
MRIYLIGFMCSGKSTVGSLLSRSLNIPFYDVDEEVQKREGLSIPQIFEKKGEAYFRKLEFEVLKDLSEKENVVISTGGGL
GANEEALNFMKSRGTTVFIDIPFEVFLERCKDSKERPLLKRPLDEIKNLFEERRKIYSKADIKVKGEKPPEEVVKEILLS
LEGNALGG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=167, Percent_Identity=36.5269461077844, Blast_Score=110, Evalue=4e-26,
Organism=Escherichia coli, GI1786587, Length=165, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_AQUAE (O67925)

Other databases:

- EMBL:   AE000657
- PIR:   A70487
- RefSeq:   NP_214494.1
- PDB:   2PT5
- PDBsum:   2PT5
- ProteinModelPortal:   O67925
- SMR:   O67925
- GeneID:   1193914
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2177
- NMPDR:   fig|224324.1.peg.1509
- HOGENOM:   HBG335815
- OMA:   MSISAFF
- PhylomeDB:   O67925
- ProtClustDB:   CLSK2748574
- BioCyc:   AAEO224324:AQ_2177-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19080; Mature: 19080

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 32-32 BINDING 56-56 BINDING 78-78 BINDING 116-116 BINDING 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIYLIGFMCSGKSTVGSLLSRSLNIPFYDVDEEVQKREGLSIPQIFEKKGEAYFRKLEF
CEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EVLKDLSEKENVVISTGGGLGANEEALNFMKSRGTTVFIDIPFEVFLERCKDSKERPLLK
HHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCHHH
RPLDEIKNLFEERRKIYSKADIKVKGEKPPEEVVKEILLSLEGNALGG
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIYLIGFMCSGKSTVGSLLSRSLNIPFYDVDEEVQKREGLSIPQIFEKKGEAYFRKLEF
CEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EVLKDLSEKENVVISTGGGLGANEEALNFMKSRGTTVFIDIPFEVFLERCKDSKERPLLK
HHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCCHHH
RPLDEIKNLFEERRKIYSKADIKVKGEKPPEEVVKEILLSLEGNALGG
CHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320