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The map label for this gene is hemB

Identifier: 15607063

GI number: 15607063

Start: 1485819

End: 1486811

Strand: Direct

Name: hemB

Synonym: aq_2109

Alternate gene names: 15607063

Gene position: 1485819-1486811 (Clockwise)

Preceding gene: 15607062

Following gene: 15607064

Centisome position: 95.78

GC content: 48.24

Gene sequence:

>993_bases
ATGATTGTAGGAAGATTTCCCAGTTCAAGACCGAGAAGGCTCAGGAGAAACGAAAACATAAGGAGGCTCGTGAGGGAAAC
CCACCTTACCCTTGACGACCTAATATACCCCATATTCGTGAGGTGGGGCGAGAACGTAGTTGAAGAAGTTCCCTCCATGC
CCGGCGTTTACAGGTACAGTGTAGACAAGGTAGTTGACGCGGTAAAGGAAGTGAGAGATTTAGGAATTCCTGCGATAATT
CTCTTCGGTATTCCCGAACATAAAGATGAAGTTGGTTCGGACACGTGGAGTGAAGAGGGGATAATTCAGAGAGCTTTGAG
GAAGATAAAGAAGGAAGTTCCGGACATATACGTGATTACGGACGTTTGTTTTTGTGAATACACAACTCACGGACACTGCG
GAGTTCTTCACGACCACGACGTGGAAAACGACCTCACCCTTGAAAACTTAAAAAAGCAGGTGGTTTCCCATGCGGAAAAC
GGGGCGGACATGGTGGCACCTTCGGGAATGATGGACGGAATGGTAAAGGTTATAAGGGAAGCTCTAGACGAAGCTGGCTT
TAAACACATACCTATAATGGCATACTCCGCCAAATTTGCCTCCGCTTACTACGGACCCTTCAGGGAAGCCGCGGAGAGTG
CTCCCGCCTTTGGAGACAGGAGAACTTATCAAATGGATCCTGCAAACTCAAGGGAAGCCCTAAAAGAGGTCCTGATGGAC
GTTGAAGAGGGTGCAGATGTAGTTATGGTAAAACCCGCCCTCGCTTACCTCGACATAATAGCGAAGGTCAAGGAAAACGT
TCTGGTTCCCGTGTGCGCTTACAACGTGAGCGGTGAGTACTCCCTTATAAAGGCCGCGGGAAAACTTGGGTGGATTGACG
AGAAGAAGGTTATGTGGGAAACACTCCTTTCCATAAAGAGAGCAGGAGCGGATATGATAATAACTTACTTTGCGAAGGAC
GCGGCGAGAATGATCATTGAGGGTGAGGTATGA

Upstream 100 bases:

>100_bases
GAAGATGCCTACATAACCGCTCTTCTTTTCCTAAAGTACGTTTACCCGAACAGGGAGTACAGACTAAAGGATCTCCCGAT
TTTCCTTTAAATTAATACTT

Downstream 100 bases:

>100_bases
AAAAAGTCGGATTTATTTACGACGATATTTACTTGAAGCACGACTGGCCGGAACACCCGGAAAACAAAAACAGGCTTATC
TCAATCTTAGAACACGTGGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MIVGRFPSSRPRRLRRNENIRRLVRETHLTLDDLIYPIFVRWGENVVEEVPSMPGVYRYSVDKVVDAVKEVRDLGIPAII
LFGIPEHKDEVGSDTWSEEGIIQRALRKIKKEVPDIYVITDVCFCEYTTHGHCGVLHDHDVENDLTLENLKKQVVSHAEN
GADMVAPSGMMDGMVKVIREALDEAGFKHIPIMAYSAKFASAYYGPFREAAESAPAFGDRRTYQMDPANSREALKEVLMD
VEEGADVVMVKPALAYLDIIAKVKENVLVPVCAYNVSGEYSLIKAAGKLGWIDEKKVMWETLLSIKRAGADMIITYFAKD
AARMIIEGEV

Sequences:

>Translated_330_residues
MIVGRFPSSRPRRLRRNENIRRLVRETHLTLDDLIYPIFVRWGENVVEEVPSMPGVYRYSVDKVVDAVKEVRDLGIPAII
LFGIPEHKDEVGSDTWSEEGIIQRALRKIKKEVPDIYVITDVCFCEYTTHGHCGVLHDHDVENDLTLENLKKQVVSHAEN
GADMVAPSGMMDGMVKVIREALDEAGFKHIPIMAYSAKFASAYYGPFREAAESAPAFGDRRTYQMDPANSREALKEVLMD
VEEGADVVMVKPALAYLDIIAKVKENVLVPVCAYNVSGEYSLIKAAGKLGWIDEKKVMWETLLSIKRAGADMIITYFAKD
AARMIIEGEV
>Mature_330_residues
MIVGRFPSSRPRRLRRNENIRRLVRETHLTLDDLIYPIFVRWGENVVEEVPSMPGVYRYSVDKVVDAVKEVRDLGIPAII
LFGIPEHKDEVGSDTWSEEGIIQRALRKIKKEVPDIYVITDVCFCEYTTHGHCGVLHDHDVENDLTLENLKKQVVSHAEN
GADMVAPSGMMDGMVKVIREALDEAGFKHIPIMAYSAKFASAYYGPFREAAESAPAFGDRRTYQMDPANSREALKEVLMD
VEEGADVVMVKPALAYLDIIAKVKENVLVPVCAYNVSGEYSLIKAAGKLGWIDEKKVMWETLLSIKRAGADMIITYFAKD
AARMIIEGEV

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=296, Percent_Identity=42.2297297297297, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI87081728, Length=314, Percent_Identity=53.5031847133758, Blast_Score=341, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6321398, Length=307, Percent_Identity=39.7394136807818, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI21358291, Length=289, Percent_Identity=41.1764705882353, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_AQUAE (O67876)

Other databases:

- EMBL:   AE000657
- PIR:   H70480
- RefSeq:   NP_214445.1
- ProteinModelPortal:   O67876
- SMR:   O67876
- GeneID:   1193865
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_2109
- NMPDR:   fig|224324.1.peg.1460
- HOGENOM:   HBG285270
- OMA:   ADLCFCE
- PhylomeDB:   O67876
- ProtClustDB:   PRK09283
- BioCyc:   AAEO224324:AQ_2109-MONOMER
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 37091; Mature: 37091

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 198-198 ACT_SITE 251-251 BINDING 208-208 BINDING 220-220 BINDING 277-277 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGRFPSSRPRRLRRNENIRRLVRETHLTLDDLIYPIFVRWGENVVEEVPSMPGVYRYS
CEECCCCCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VDKVVDAVKEVRDLGIPAIILFGIPEHKDEVGSDTWSEEGIIQRALRKIKKEVPDIYVIT
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
DVCFCEYTTHGHCGVLHDHDVENDLTLENLKKQVVSHAENGADMVAPSGMMDGMVKVIRE
HHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ALDEAGFKHIPIMAYSAKFASAYYGPFREAAESAPAFGDRRTYQMDPANSREALKEVLMD
HHHHCCCCCCCEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHH
VEEGADVVMVKPALAYLDIIAKVKENVLVPVCAYNVSGEYSLIKAAGKLGWIDEKKVMWE
HHCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHH
TLLSIKRAGADMIITYFAKDAARMIIEGEV
HHHHHHHCCCHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MIVGRFPSSRPRRLRRNENIRRLVRETHLTLDDLIYPIFVRWGENVVEEVPSMPGVYRYS
CEECCCCCCCCHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VDKVVDAVKEVRDLGIPAIILFGIPEHKDEVGSDTWSEEGIIQRALRKIKKEVPDIYVIT
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
DVCFCEYTTHGHCGVLHDHDVENDLTLENLKKQVVSHAENGADMVAPSGMMDGMVKVIRE
HHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ALDEAGFKHIPIMAYSAKFASAYYGPFREAAESAPAFGDRRTYQMDPANSREALKEVLMD
HHHHCCCCCCCEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHH
VEEGADVVMVKPALAYLDIIAKVKENVLVPVCAYNVSGEYSLIKAAGKLGWIDEKKVMWE
HHCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHH
TLLSIKRAGADMIITYFAKDAARMIIEGEV
HHHHHHHCCCHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320