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The map label for this gene is spoT [H]

Identifier: 15602785

GI number: 15602785

Start: 1085617

End: 1087740

Strand: Reverse

Name: spoT [H]

Synonym: PM0920

Alternate gene names: 15602785

Gene position: 1087740-1085617 (Counterclockwise)

Preceding gene: 15602786

Following gene: 15602784

Centisome position: 48.18

GC content: 40.21

Gene sequence:

>2124_bases
TTGTATCTTTTTGAAAGTCTAAATCGCATTATTGAGGCGTATTTACCGCCAGAACAAATCGAACTTGTTAAACGTGCGTA
TATTATTGCTCGTGATGCGCATGAGGGACAATCGCGTTCTAGTGGTGAACCTTATATCACTCATCCTGTCGCAGTCGCAT
CGATTATTGCTGAAATGCGTTTAGATCATGAAGCTGTGATGGCGGCATTATTGCATGATGTAATTGAAGACACCCCTTAT
ACAGAAGAGCAATTGAAAACAGAGTTTGGTGCCAGTGTGGCAGAAATTGTAGAAGGCGTATCAAAACTCGATAAGTTGAA
ATTCCGGACACGTAAAGAGGCGGCAGCTGAAAACTTTCGCAAAATGATTTTAGCCATGACACGAGATATTCGTGTGGTGT
TAATTAAGCTTGCTGATAGAACGCACAATATGCGTACACTCGGCTCGCTACGTCCAGATAAACGCCGCCGTATTGCAAAA
GAAACGCTTGAAATTTATAGTCCTTTGGCTCATCGTTTAGGGATTGAACATATCAAAAACGAGTTGGAAGACTTAGGCTT
TGAAGCCATGCACCCACAACGTTATGCGGTGTTGCAAAAAGTGATTCAGGTGGCGCGTGGTAATCGTAAAGACATGATTC
AGCGTATTACCAATGAAATTAAAGGACGATTAGAAGATGTGGGCATTAAAGCTCGTGTTTTTGGGCGGGAAAAACATCTT
TATGCCATTTATCAAAAAATGAAACTGAAAGATCAACAGTTTCATTCTATTATGGATATCTATGCATTCCGTGCTGTCGT
TAAAGATATTGATACTTGCTATCGTGTGCTTGGGCAAATGCATGGGTTATATAAACCTCGTCCTGGTCGTGTTAAAGATT
ACATCGCGGTGCCAAAAGCAAATGGCTACCAGTCATTACATACCTCAATGATCGGTCCACATGGTGTTCCCGTTGAAGTA
CAAATTCGTACTGAAGAAATGGATCAAATGGCGGAAATGGGGGTAGCAGCTCATTGGGCATATAAAGAAGGTGGCAAAAA
TGACAGCACGACAGTACAAATTCGAGCTCAACGTTGGTTACAAAGCTTAATTGAATTGCAACAAAGTGTGGGAAATTCTT
TTGAGTTTATCGAGAGTGTCAAATCTGAATTTTTCCCAAAAGAGATTTATGTTTTCACACCGAAAGGGCGTATTGTGGAA
TTGCCAGTTGGTGCAACGCCTGTCGATTTTGCTTATGCGGTACACACGGATATCGGTCATAGCTGTATTGCGGCTAATGT
TGATCGTAAATCTTACCCTTTATCACAAGCCTTAACTTCAGGACAAACGGTTGACATCATTACTTTACCAAATTCTCAAC
CGAGCGCGGGATGGTTAAATTTTGTGGTGACAGCGAAAGCACGAACCAATATTCGTAATTTCCTTAAAAATTTACGTGGT
GGTGAGGCGATAACATTAGGTAAACGTCAGTTGACACGTGCGTTGGCACCGACGAAGTTAGAAGATCTTGATAAAAATAG
AGTACAAAATTTATTAACTGAGCTGAAATTAGCCACATTCGATGATTTATTAATGGAAATTGGTCTAGGTAATCAAATGA
GTGCGGTAATCGCTTATCGCTTGCTAGGAGAAGCCATTGAAATTGATACGGATGGTGATCTTACAAACAATAAAGCGAAT
TTTGAAATTAAAAGTGCTGGTGGATTACTTACCACGTTTGCCCAATGTTGTCACCCAATTCCAGGCGATCCTATTGTTGC
TTATACGAGCTCCGGCAAGGGCTTAGTGATTCATCATGAAGCTTGCGCGAATTTAAAACATCGTAAAGACAATCCTGAAC
ACTATATGCCAGTGGAATGGGAGCAAAGCGATAATATGATCGATTTTGAAGCGGAATTGCGTATTGAACTTATCAATCAG
CAAGGCGCTTTACCAAATTTAACGACACTGATTTCGTCTTTAGGCAGTAATATTCAAAGTATTTGGACGGAAGAACAAGA
AGGGCGTTTATATCAAATCGTCGTGTTGTTGACCGTAAAAGACACTAAGCATTTAGCGAATATTATTCGTAAAATCAAAG
CAATTCCGGGTTTTGTTAATATTGAACGTAATATCAATCAATGA

Upstream 100 bases:

>100_bases
CTCGGAATTACACGCTGTTTCTTTATTATCTAATCAGTAAAAGTGCGGTCATTTTGCACAACGTTTTCAGAAAATCACAT
TGAACTAAGTTAGGTGTCCC

Downstream 100 bases:

>100_bases
CCATACAATTCCTCGATGCAGTCCCTTTAACGACACTTTCTGGTGTTGGGGCTGCCATTTCTGACAAGCTTGGGCGGATT
GGTATTCACAATCTCCAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 707; Mature: 707

Protein sequence:

>707_residues
MYLFESLNRIIEAYLPPEQIELVKRAYIIARDAHEGQSRSSGEPYITHPVAVASIIAEMRLDHEAVMAALLHDVIEDTPY
TEEQLKTEFGASVAEIVEGVSKLDKLKFRTRKEAAAENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYAVLQKVIQVARGNRKDMIQRITNEIKGRLEDVGIKARVFGREKHL
YAIYQKMKLKDQQFHSIMDIYAFRAVVKDIDTCYRVLGQMHGLYKPRPGRVKDYIAVPKANGYQSLHTSMIGPHGVPVEV
QIRTEEMDQMAEMGVAAHWAYKEGGKNDSTTVQIRAQRWLQSLIELQQSVGNSFEFIESVKSEFFPKEIYVFTPKGRIVE
LPVGATPVDFAYAVHTDIGHSCIAANVDRKSYPLSQALTSGQTVDIITLPNSQPSAGWLNFVVTAKARTNIRNFLKNLRG
GEAITLGKRQLTRALAPTKLEDLDKNRVQNLLTELKLATFDDLLMEIGLGNQMSAVIAYRLLGEAIEIDTDGDLTNNKAN
FEIKSAGGLLTTFAQCCHPIPGDPIVAYTSSGKGLVIHHEACANLKHRKDNPEHYMPVEWEQSDNMIDFEAELRIELINQ
QGALPNLTTLISSLGSNIQSIWTEEQEGRLYQIVVLLTVKDTKHLANIIRKIKAIPGFVNIERNINQ

Sequences:

>Translated_707_residues
MYLFESLNRIIEAYLPPEQIELVKRAYIIARDAHEGQSRSSGEPYITHPVAVASIIAEMRLDHEAVMAALLHDVIEDTPY
TEEQLKTEFGASVAEIVEGVSKLDKLKFRTRKEAAAENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYAVLQKVIQVARGNRKDMIQRITNEIKGRLEDVGIKARVFGREKHL
YAIYQKMKLKDQQFHSIMDIYAFRAVVKDIDTCYRVLGQMHGLYKPRPGRVKDYIAVPKANGYQSLHTSMIGPHGVPVEV
QIRTEEMDQMAEMGVAAHWAYKEGGKNDSTTVQIRAQRWLQSLIELQQSVGNSFEFIESVKSEFFPKEIYVFTPKGRIVE
LPVGATPVDFAYAVHTDIGHSCIAANVDRKSYPLSQALTSGQTVDIITLPNSQPSAGWLNFVVTAKARTNIRNFLKNLRG
GEAITLGKRQLTRALAPTKLEDLDKNRVQNLLTELKLATFDDLLMEIGLGNQMSAVIAYRLLGEAIEIDTDGDLTNNKAN
FEIKSAGGLLTTFAQCCHPIPGDPIVAYTSSGKGLVIHHEACANLKHRKDNPEHYMPVEWEQSDNMIDFEAELRIELINQ
QGALPNLTTLISSLGSNIQSIWTEEQEGRLYQIVVLLTVKDTKHLANIIRKIKAIPGFVNIERNINQ
>Mature_707_residues
MYLFESLNRIIEAYLPPEQIELVKRAYIIARDAHEGQSRSSGEPYITHPVAVASIIAEMRLDHEAVMAALLHDVIEDTPY
TEEQLKTEFGASVAEIVEGVSKLDKLKFRTRKEAAAENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYAVLQKVIQVARGNRKDMIQRITNEIKGRLEDVGIKARVFGREKHL
YAIYQKMKLKDQQFHSIMDIYAFRAVVKDIDTCYRVLGQMHGLYKPRPGRVKDYIAVPKANGYQSLHTSMIGPHGVPVEV
QIRTEEMDQMAEMGVAAHWAYKEGGKNDSTTVQIRAQRWLQSLIELQQSVGNSFEFIESVKSEFFPKEIYVFTPKGRIVE
LPVGATPVDFAYAVHTDIGHSCIAANVDRKSYPLSQALTSGQTVDIITLPNSQPSAGWLNFVVTAKARTNIRNFLKNLRG
GEAITLGKRQLTRALAPTKLEDLDKNRVQNLLTELKLATFDDLLMEIGLGNQMSAVIAYRLLGEAIEIDTDGDLTNNKAN
FEIKSAGGLLTTFAQCCHPIPGDPIVAYTSSGKGLVIHHEACANLKHRKDNPEHYMPVEWEQSDNMIDFEAELRIELINQ
QGALPNLTTLISSLGSNIQSIWTEEQEGRLYQIVVLLTVKDTKHLANIIRKIKAIPGFVNIERNINQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=67.2316384180791, Blast_Score=1002, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=694, Percent_Identity=31.9884726224784, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI25143118, Length=141, Percent_Identity=34.7517730496454, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79832; Mature: 79832

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLFESLNRIIEAYLPPEQIELVKRAYIIARDAHEGQSRSSGEPYITHPVAVASIIAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKTEFGASVAEIVEGVSKLDKLKFRTRKEAAAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYSPLAHRLGIEHIKN
HHHHHHHCCHHHHHEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEDLGFEAMHPQRYAVLQKVIQVARGNRKDMIQRITNEIKGRLEDVGIKARVFGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHH
YAIYQKMKLKDQQFHSIMDIYAFRAVVKDIDTCYRVLGQMHGLYKPRPGRVKDYIAVPKA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEEMDQMAEMGVAAHWAYKEGGKNDSTTVQIRAQRWL
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHEEHHHCCCCCCCCEEEEHHHHHH
QSLIELQQSVGNSFEFIESVKSEFFPKEIYVFTPKGRIVELPVGATPVDFAYAVHTDIGH
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCHHEEEECCCCC
SCIAANVDRKSYPLSQALTSGQTVDIITLPNSQPSAGWLNFVVTAKARTNIRNFLKNLRG
CCEEECCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCC
GEAITLGKRQLTRALAPTKLEDLDKNRVQNLLTELKLATFDDLLMEIGLGNQMSAVIAYR
CCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGEAIEIDTDGDLTNNKANFEIKSAGGLLTTFAQCCHPIPGDPIVAYTSSGKGLVIHHE
HHHHHEEECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEECH
ACANLKHRKDNPEHYMPVEWEQSDNMIDFEAELRIELINQQGALPNLTTLISSLGSNIQS
HHHCCCCCCCCCCCCCCCEECCCCCEEEEEHHEEEEEECCCCCCCHHHHHHHHHCCHHHH
IWTEEQEGRLYQIVVLLTVKDTKHLANIIRKIKAIPGFVNIERNINQ
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC
>Mature Secondary Structure
MYLFESLNRIIEAYLPPEQIELVKRAYIIARDAHEGQSRSSGEPYITHPVAVASIIAEMR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCCCCCCCCCEEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKTEFGASVAEIVEGVSKLDKLKFRTRKEAAAENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYSPLAHRLGIEHIKN
HHHHHHHCCHHHHHEEHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
ELEDLGFEAMHPQRYAVLQKVIQVARGNRKDMIQRITNEIKGRLEDVGIKARVFGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHH
YAIYQKMKLKDQQFHSIMDIYAFRAVVKDIDTCYRVLGQMHGLYKPRPGRVKDYIAVPKA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCC
NGYQSLHTSMIGPHGVPVEVQIRTEEMDQMAEMGVAAHWAYKEGGKNDSTTVQIRAQRWL
CCHHHHHHHHCCCCCCCEEEEECHHHHHHHHHCCHHHEEHHHCCCCCCCCEEEEHHHHHH
QSLIELQQSVGNSFEFIESVKSEFFPKEIYVFTPKGRIVELPVGATPVDFAYAVHTDIGH
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCCHHEEEECCCCC
SCIAANVDRKSYPLSQALTSGQTVDIITLPNSQPSAGWLNFVVTAKARTNIRNFLKNLRG
CCEEECCCCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCC
GEAITLGKRQLTRALAPTKLEDLDKNRVQNLLTELKLATFDDLLMEIGLGNQMSAVIAYR
CCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGEAIEIDTDGDLTNNKANFEIKSAGGLLTTFAQCCHPIPGDPIVAYTSSGKGLVIHHE
HHHHHEEECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCEEEEECH
ACANLKHRKDNPEHYMPVEWEQSDNMIDFEAELRIELINQQGALPNLTTLISSLGSNIQS
HHHCCCCCCCCCCCCCCCEECCCCCEEEEEHHEEEEEECCCCCCCHHHHHHHHHCCHHHH
IWTEEQEGRLYQIVVLLTVKDTKHLANIIRKIKAIPGFVNIERNINQ
HHCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]