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The map label for this gene is truB

Identifier: 15602621

GI number: 15602621

Start: 891013

End: 891936

Strand: Reverse

Name: truB

Synonym: PM0756

Alternate gene names: 15602621

Gene position: 891936-891013 (Counterclockwise)

Preceding gene: 15602622

Following gene: 15602618

Centisome position: 39.51

GC content: 43.61

Gene sequence:

>924_bases
ATGGCAAAGCCGCGCAAACGTGGACGTGATATTGACGGCGTTTTTTTGCTAGATAAGCCTCAAGGGATGAGCTCCAATGA
TATTATGCAAAAGGTCAAACGTGTGTTTCAAGCTAACAAAGCAGGACACACAGGTGCATTAGATCCTTTGGCAACGGGGA
TGTTGCCAATTTGCTTAGGTGAAGCCACGAAGTTTTCACAATTTTTGTTGGATGCGGATAAACGTTATCAAGTGACCGCG
AAACTTGGGGAAAGAACCGATACGTCCGATGCGGAAGGGCAAGTCGTTGAAACACGAGATGTGCAGGTAGATGTGCAGGA
CATTTTGGCTGCACTACCACATTTTCGTGGCAATTTAATGCAAGTTCCCACGATGTTTTCTGCGTTAAAACATCAAGGTA
AGCCTTTATATGAGTATGCACGTGCTGGCATAACAGTGGAACGAGAAGCTCGCCCGATCACGATTTTTGACTTGCAATTT
ATTGCGTACGATGCCCCTTATTTAACGCTGGAAGTACATTGTTCAAAAGGCACTTATATACGCACTTTAGTGGATGATTT
AGGCGAATATTTGGGCTGTGGTGCACATGTAACGGTGTTACGTCGAACCGCTGTTGCCAATTATCCTGTGGAGGCGATGA
TGAACTGGGACACTTTACAAGTTTTAGCTGCACAACAAGACCTCGCATTATTAGATCAGCACTTATTACCGACAGACAGT
GCGGTCTCCGCATTACCTGCATTGCATTTAAATCAAGAACAAAGTAAAGCCATTAGTTTTGGTCAACGTGTCAAGTTTGA
CAATCCAACTCAGCTTACTGGACAAGTTCGTTTATTTTCTGACACGCAACAATTTTTAGGTGTTGCCTTAGTCGATGAGC
ATAATGTCATTCGTCCGCAACGTTTGATGACACAAAATACGTAA

Upstream 100 bases:

>100_bases
GCTCTTTGATTGAAGGAATGCGAATGTCTAATTTAGTCAGCAATGTGATTCGCGAAGATAATGCGCGTCATATTGATGAC
GATGTTTCAGAGGAAAAGTA

Downstream 100 bases:

>100_bases
ATATTTGAGAAAAGAAAATCCACCGTACTTTTCACGATGAAGCTAAAGTGCGGTGGATTTTTTATTTATTTTTAAGTATG
AACGCTTACTTCACCGTCAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAKPRKRGRDIDGVFLLDKPQGMSSNDIMQKVKRVFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYQVTA
KLGERTDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLMQVPTMFSALKHQGKPLYEYARAGITVEREARPITIFDLQF
IAYDAPYLTLEVHCSKGTYIRTLVDDLGEYLGCGAHVTVLRRTAVANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDS
AVSALPALHLNQEQSKAISFGQRVKFDNPTQLTGQVRLFSDTQQFLGVALVDEHNVIRPQRLMTQNT

Sequences:

>Translated_307_residues
MAKPRKRGRDIDGVFLLDKPQGMSSNDIMQKVKRVFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYQVTA
KLGERTDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLMQVPTMFSALKHQGKPLYEYARAGITVEREARPITIFDLQF
IAYDAPYLTLEVHCSKGTYIRTLVDDLGEYLGCGAHVTVLRRTAVANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDS
AVSALPALHLNQEQSKAISFGQRVKFDNPTQLTGQVRLFSDTQQFLGVALVDEHNVIRPQRLMTQNT
>Mature_306_residues
AKPRKRGRDIDGVFLLDKPQGMSSNDIMQKVKRVFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDADKRYQVTAK
LGERTDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLMQVPTMFSALKHQGKPLYEYARAGITVEREARPITIFDLQFI
AYDAPYLTLEVHCSKGTYIRTLVDDLGEYLGCGAHVTVLRRTAVANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDSA
VSALPALHLNQEQSKAISFGQRVKFDNPTQLTGQVRLFSDTQQFLGVALVDEHNVIRPQRLMTQNT

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=220, Percent_Identity=35.4545454545455, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI4503337, Length=174, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI215599015, Length=174, Percent_Identity=31.0344827586207, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI2367200, Length=312, Percent_Identity=57.3717948717949, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553978, Length=292, Percent_Identity=24.6575342465753, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=31.6062176165803, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=293, Percent_Identity=25.938566552901, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364189, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364187, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364185, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364183, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI62471759, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17975520, Length=192, Percent_Identity=29.6875, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_PASMU (Q9CMQ7)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245693.1
- ProteinModelPortal:   Q9CMQ7
- SMR:   Q9CMQ7
- GeneID:   1244103
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0756
- NMPDR:   fig|272843.1.peg.756
- HOGENOM:   HBG397258
- OMA:   ERTISEP
- ProtClustDB:   PRK05033
- BioCyc:   PMUL272843:PM0756-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34153; Mature: 34021

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPRKRGRDIDGVFLLDKPQGMSSNDIMQKVKRVFQANKAGHTGALDPLATGMLPICLG
CCCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYQVTAKLGERTDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLM
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCEE
QVPTMFSALKHQGKPLYEYARAGITVEREARPITIFDLQFIAYDAPYLTLEVHCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEYLGCGAHVTVLRRTAVANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
AVSALPALHLNQEQSKAISFGQRVKFDNPTQLTGQVRLFSDTQQFLGVALVDEHNVIRPQ
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHEEEEECCCCCCCCH
RLMTQNT
HHHCCCC
>Mature Secondary Structure 
AKPRKRGRDIDGVFLLDKPQGMSSNDIMQKVKRVFQANKAGHTGALDPLATGMLPICLG
CCCCHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDADKRYQVTAKLGERTDTSDAEGQVVETRDVQVDVQDILAALPHFRGNLM
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEECCCEECHHHHHHHHHHHCCCEE
QVPTMFSALKHQGKPLYEYARAGITVEREARPITIFDLQFIAYDAPYLTLEVHCSKGTYI
HHHHHHHHHHHCCCHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCEEEEEEEECCCCCH
RTLVDDLGEYLGCGAHVTVLRRTAVANYPVEAMMNWDTLQVLAAQQDLALLDQHLLPTDS
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
AVSALPALHLNQEQSKAISFGQRVKFDNPTQLTGQVRLFSDTQQFLGVALVDEHNVIRPQ
HHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHEEEEECCCCCCCCH
RLMTQNT
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100