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The map label for this gene is dacB [H]

Identifier: 15602581

GI number: 15602581

Start: 841385

End: 842848

Strand: Direct

Name: dacB [H]

Synonym: PM0716

Alternate gene names: 15602581

Gene position: 841385-842848 (Clockwise)

Preceding gene: 15602578

Following gene: 15602582

Centisome position: 37.27

GC content: 39.69

Gene sequence:

>1464_bases
ATGTATAAAATATCTTCTTTTTTTACTGCCACGCTTTTACGCCTTTTCAAACGAACGATACTTATATGTAGTTTACTCGT
CAGTCATTTCAGTTATGCTGAGATTGATCTTGCTGAAATCAACGCTCATTTACCCGAAGGGACCAGCCTAGGTTTTGTGG
CTAAAAACTTAAATCGTAATGAAAAAATTGTAGAACACCAAGCCAGTACTTTTATGTTACCCGCCAGTACACAAAAAGTG
CTCACCGCCTTAACCGCCAAACTCGTACTCAGTGACCAATTTCAATTTGAAACCAGTCTATTAACCAATGGAAAAATAAA
AAACAATGAAATGCATGGTGATCTCATCGTACGTTTTACAGGTGACCCCGATCTCACCAGTGGTCAGCTTTACCAACTTT
TCGCCCAATTAAAAAAACAGCGTATCCAAAAAATCAGTGGTGATATTATTTTAGATACCTCAGTATTTAGCAGTCATGAT
CGTGGTTTAGGTTGGATCTGGAATGACTTAACCATGTGTTTCAATGCACCGCCTGCTGCGGTAAACATTGATAATAACTG
TTTTTATGTTGAGTTAGATGCGAATTATGCACCGGGTGAGCAAGCGAAGATCCATGTTCCTGCGCAATATCCTGTACAGG
TTTTTGGTCAAGTCTATATTTCAACGCCTAAAGAGGCTGGGTATTGCCAACTTGATGCCACCGTACTTGATAATAATCGT
TACCAGTTAAAAGGATGCTTAGCACGCCAAAACAAACCCTTTGGTCTGAGTTTTGCGATCCAAGATCCGAATGCCTATGC
GGCAACCATTATTCAGCGACACCTCAAAAAATTAGGAATCGCTTTTAATGGTAAAATCAAACAGCCTTATCAGGCGCAAA
AAGGACAATTATTAGCACAGCATCTCTCTAAGCCCTTACCTGATTTAATCAAAAAAATGATGAAAAAATCAGATAACCAA
ATTGCCGATGCCTTATTCCGTACTATCGCTTATCAATATTATCAGCGCCCCGCTTCATTTCCTTTGGCTAGCCAAGCCAT
GCGACAGATTTTACAAACCAAAGCAGGAATTAAATTTGGTCATGCCATTATTGCAGATGGCTCCGGCTTATCACGTCATA
ATTTACTCTCTGCTGACATTTTATTACAAGCATTGGAATACATTGCAAAACATGAAGCACAGTTACAGCTCATGGATACT
TTTCCAATTGCGGCTGTGGATGGCACATTAACGGGACGAGGTTCTCTCATTCATGAACCCTTAGCGAAAAATTTAATTGC
CAAAACAGGCGCTCTCAAAGGTGTGTATAATCTCGCCGGCTTTATGACCAATAAAAAAGGGGAAAAAGTTGCTTTTGTGC
AATTTATTAATGGCTATTCTACTGGCGACTTTGAGCGCAAAACAAAACGGGCGCCTTTAGTTCAATTTGAAAGCCAAATC
TACAATCGCCTTTATGAACACTAA

Upstream 100 bases:

>100_bases
AATAAAAAAATAAGTGTTTAACGAGAAAAGGATTTACTCTCGAGGGTTCAAATACTATTATTGTTCGACAATAAAAAAAT
AGCAACTGCTCGAGCCAATT

Downstream 100 bases:

>100_bases
ATTAAAGCTAACGCATACTGAAGTACTACGCTAACCACTGCCTGTGTGTGAATCGAAAAGAGAGCACATCGTCTGCGTGC
CTTTCTCCTCGTTGTGTATA

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MYKISSFFTATLLRLFKRTILICSLLVSHFSYAEIDLAEINAHLPEGTSLGFVAKNLNRNEKIVEHQASTFMLPASTQKV
LTALTAKLVLSDQFQFETSLLTNGKIKNNEMHGDLIVRFTGDPDLTSGQLYQLFAQLKKQRIQKISGDIILDTSVFSSHD
RGLGWIWNDLTMCFNAPPAAVNIDNNCFYVELDANYAPGEQAKIHVPAQYPVQVFGQVYISTPKEAGYCQLDATVLDNNR
YQLKGCLARQNKPFGLSFAIQDPNAYAATIIQRHLKKLGIAFNGKIKQPYQAQKGQLLAQHLSKPLPDLIKKMMKKSDNQ
IADALFRTIAYQYYQRPASFPLASQAMRQILQTKAGIKFGHAIIADGSGLSRHNLLSADILLQALEYIAKHEAQLQLMDT
FPIAAVDGTLTGRGSLIHEPLAKNLIAKTGALKGVYNLAGFMTNKKGEKVAFVQFINGYSTGDFERKTKRAPLVQFESQI
YNRLYEH

Sequences:

>Translated_487_residues
MYKISSFFTATLLRLFKRTILICSLLVSHFSYAEIDLAEINAHLPEGTSLGFVAKNLNRNEKIVEHQASTFMLPASTQKV
LTALTAKLVLSDQFQFETSLLTNGKIKNNEMHGDLIVRFTGDPDLTSGQLYQLFAQLKKQRIQKISGDIILDTSVFSSHD
RGLGWIWNDLTMCFNAPPAAVNIDNNCFYVELDANYAPGEQAKIHVPAQYPVQVFGQVYISTPKEAGYCQLDATVLDNNR
YQLKGCLARQNKPFGLSFAIQDPNAYAATIIQRHLKKLGIAFNGKIKQPYQAQKGQLLAQHLSKPLPDLIKKMMKKSDNQ
IADALFRTIAYQYYQRPASFPLASQAMRQILQTKAGIKFGHAIIADGSGLSRHNLLSADILLQALEYIAKHEAQLQLMDT
FPIAAVDGTLTGRGSLIHEPLAKNLIAKTGALKGVYNLAGFMTNKKGEKVAFVQFINGYSTGDFERKTKRAPLVQFESQI
YNRLYEH
>Mature_487_residues
MYKISSFFTATLLRLFKRTILICSLLVSHFSYAEIDLAEINAHLPEGTSLGFVAKNLNRNEKIVEHQASTFMLPASTQKV
LTALTAKLVLSDQFQFETSLLTNGKIKNNEMHGDLIVRFTGDPDLTSGQLYQLFAQLKKQRIQKISGDIILDTSVFSSHD
RGLGWIWNDLTMCFNAPPAAVNIDNNCFYVELDANYAPGEQAKIHVPAQYPVQVFGQVYISTPKEAGYCQLDATVLDNNR
YQLKGCLARQNKPFGLSFAIQDPNAYAATIIQRHLKKLGIAFNGKIKQPYQAQKGQLLAQHLSKPLPDLIKKMMKKSDNQ
IADALFRTIAYQYYQRPASFPLASQAMRQILQTKAGIKFGHAIIADGSGLSRHNLLSADILLQALEYIAKHEAQLQLMDT
FPIAAVDGTLTGRGSLIHEPLAKNLIAKTGALKGVYNLAGFMTNKKGEKVAFVQFINGYSTGDFERKTKRAPLVQFESQI
YNRLYEH

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=476, Percent_Identity=41.3865546218487, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 54373; Mature: 54373

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKISSFFTATLLRLFKRTILICSLLVSHFSYAEIDLAEINAHLPEGTSLGFVAKNLNRN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHHHCCCCC
EKIVEHQASTFMLPASTQKVLTALTAKLVLSDQFQFETSLLTNGKIKNNEMHGDLIVRFT
HHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEC
GDPDLTSGQLYQLFAQLKKQRIQKISGDIILDTSVFSSHDRGLGWIWNDLTMCFNAPPAA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCE
VNIDNNCFYVELDANYAPGEQAKIHVPAQYPVQVFGQVYISTPKEAGYCQLDATVLDNNR
EEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCC
YQLKGCLARQNKPFGLSFAIQDPNAYAATIIQRHLKKLGIAFNGKIKQPYQAQKGQLLAQ
EEEEHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHH
HLSKPLPDLIKKMMKKSDNQIADALFRTIAYQYYQRPASFPLASQAMRQILQTKAGIKFG
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEC
HAIIADGSGLSRHNLLSADILLQALEYIAKHEAQLQLMDTFPIAAVDGTLTGRGSLIHEP
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHH
LAKNLIAKTGALKGVYNLAGFMTNKKGEKVAFVQFINGYSTGDFERKTKRAPLVQFESQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHH
YNRLYEH
HHHHHCC
>Mature Secondary Structure
MYKISSFFTATLLRLFKRTILICSLLVSHFSYAEIDLAEINAHLPEGTSLGFVAKNLNRN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHHHCCCCC
EKIVEHQASTFMLPASTQKVLTALTAKLVLSDQFQFETSLLTNGKIKNNEMHGDLIVRFT
HHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEEC
GDPDLTSGQLYQLFAQLKKQRIQKISGDIILDTSVFSSHDRGLGWIWNDLTMCFNAPPAA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCE
VNIDNNCFYVELDANYAPGEQAKIHVPAQYPVQVFGQVYISTPKEAGYCQLDATVLDNNR
EEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCEEEEECCCCCCEEEEEEEEECCCC
YQLKGCLARQNKPFGLSFAIQDPNAYAATIIQRHLKKLGIAFNGKIKQPYQAQKGQLLAQ
EEEEHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHH
HLSKPLPDLIKKMMKKSDNQIADALFRTIAYQYYQRPASFPLASQAMRQILQTKAGIKFG
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEC
HAIIADGSGLSRHNLLSADILLQALEYIAKHEAQLQLMDTFPIAAVDGTLTGRGSLIHEP
CEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCHHHHH
LAKNLIAKTGALKGVYNLAGFMTNKKGEKVAFVQFINGYSTGDFERKTKRAPLVQFESQI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHH
YNRLYEH
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]