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The map label for this gene is ackA

Identifier: 15602569

GI number: 15602569

Start: 819907

End: 821112

Strand: Direct

Name: ackA

Synonym: PM0704

Alternate gene names: 15602569

Gene position: 819907-821112 (Clockwise)

Preceding gene: 15602564

Following gene: 15602570

Centisome position: 36.32

GC content: 40.46

Gene sequence:

>1206_bases
ATGTCTCAAAAATTAGTCCTAATCTTAAACTGTGGTAGTTCTTCATTAAAATTCTCTATTTTGGACCCGCAAACTGGTGA
AGAAAAATTGTCTGGTTTAGCCGAAGCATTTCATTTAGATGATGCGCGTATTAAATGGAAACTCCATGGTGAAAAAGGTA
ACGCTGATTTAGGTGCAGGTGCTGCACATAGTGAAGCACTTAACTTTATTGTAAATAGTATTTTCCCATTAGATCCATCT
TTAAAAGAAGACATTGTTGCGATCGGTCACCGTATCGTTCATGGCGGTGAAAAATTCACGTCTTCTGTCGTCATTACGGA
TGAAGTGGTTCAAGGCATTAAAGACGCTGTTCAATTCGCCCCATTACACAACCCAGCACATTTAATCGGTATTGAAGAAG
CCTTCAAAATGTTCCCTCATTTAAAAGATAAAAATGTGGCTGTCTTTGATACCGCTTTCCATCAAACCATGCCTGAGGAA
GCTTATTTATATGCGCTACCTTATTCTTTATATAGAGAACATGGTGTACGTCGTTATGGCGCACATGGTACAAGTCATTT
CTTTGTTAGCCAGCAAGCCGCTGAACGTCTAAATGTTCCGGCAGAACAAGTAAATGTCATTACCTGTCACTTAGGTAATG
GTGCCTCTATCGCGGCAGTACGTCATGGACAATGTATTGACACCTCTATGGGCTTAACGCCATTAGAAGGTTTAGTCATG
GGAACACGTTCTGGTGATATTGATCCAGCAATCGTCTTTTATTTACACGACAACCTTGGTTTATCTGTTGAAGAGATCAA
TACATTATTAACCAAAAAATCAGGCTTACTCGGTTTAACCGAAGTCACAAGCGACTGTCGTTATGCAGAAGATAACTATG
ACAAAGAAGCGTCAGCAAAACGTGCATTAGACGTATTTAGCTATCGTTTAGCAAAATATATTGGTTCTTACATGGCGGTT
ATCGGTGAACGTTTAGATGCTATCGTCTTCACGGGTGGTATTGGTGAAAACTCATCTTTAGTACGCGAGTTAACCTTAAA
CCATTTAAAACTGTTCGGTTATCAAGTGGATTCAGATAAAAACAAAGCGGCTCGCTTTGGTCATGAAGGGGTGATTACAG
CAGACAATACGCCTGTTGCCATGGTGATCCCAACGAATGAAGAACTTGTTATTGCACAAGATACCGCGCGCCTTTGCATC
GCGTAA

Upstream 100 bases:

>100_bases
CGAAAGGTGTATCCTATGCCTATCAGATTTTCATCTGCCTATTTGTTCAATATTTGAGCAAATAACTTTTTTTAACTTAA
AATCAAATAATAGGTCTCTT

Downstream 100 bases:

>100_bases
AGATCAATTAACTGCCGATTCATCGGCAGTTTCTTTTTGTATAAATTATCAACAAATTAGGGTTTATTATGTCACGTACA
ATTATCCTTATCCCGATTAG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAGAAHSEALNFIVNSIFPLDPS
LKEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFAPLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEE
AYLYALPYSLYREHGVRRYGAHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVM
GTRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAKRALDVFSYRLAKYIGSYMAV
IGERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDKNKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCI
A

Sequences:

>Translated_401_residues
MSQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAGAAHSEALNFIVNSIFPLDPS
LKEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFAPLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEE
AYLYALPYSLYREHGVRRYGAHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVM
GTRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAKRALDVFSYRLAKYIGSYMAV
IGERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDKNKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCI
A
>Mature_400_residues
SQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAGAAHSEALNFIVNSIFPLDPSL
KEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFAPLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEEA
YLYALPYSLYREHGVRRYGAHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVMG
TRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAKRALDVFSYRLAKYIGSYMAVI
GERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDKNKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCIA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=72.1105527638191, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI145693184, Length=397, Percent_Identity=44.8362720403023, Blast_Score=329, Evalue=2e-91,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_PASMU (P57866)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245641.1
- PDB:   1LP2
- PDBsum:   1LP2
- ProteinModelPortal:   P57866
- SMR:   P57866
- PRIDE:   P57866
- GeneID:   1244051
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0704
- NMPDR:   fig|272843.1.peg.704
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- BioCyc:   PMUL272843:PM0704-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43691; Mature: 43560

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAG
CCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
AAHSEALNFIVNSIFPLDPSLKEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFA
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCHHHHCEEEEEHHHHHHHHHHHHHC
PLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEEAYLYALPYSLYREHGVRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCEEEEECCHHHHHHCCCHHHC
AHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCCEECCCCCCCCCCCEEE
GTRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAK
ECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RALDVFSYRLAKYIGSYMAVIGERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCIA
CHHHHCCCCCEEEECCCCEEEEEECCCCEEEEECCCHHCCC
>Mature Secondary Structure 
SQKLVLILNCGSSSLKFSILDPQTGEEKLSGLAEAFHLDDARIKWKLHGEKGNADLGAG
CCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
AAHSEALNFIVNSIFPLDPSLKEDIVAIGHRIVHGGEKFTSSVVITDEVVQGIKDAVQFA
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCHHHHCEEEEEHHHHHHHHHHHHHC
PLHNPAHLIGIEEAFKMFPHLKDKNVAVFDTAFHQTMPEEAYLYALPYSLYREHGVRRYG
CCCCCHHEEEHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCEEEEECCHHHHHHCCCHHHC
AHGTSHFFVSQQAAERLNVPAEQVNVITCHLGNGASIAAVRHGQCIDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCCEECCCCCCCCCCCEEE
GTRSGDIDPAIVFYLHDNLGLSVEEINTLLTKKSGLLGLTEVTSDCRYAEDNYDKEASAK
ECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCHHHHH
RALDVFSYRLAKYIGSYMAVIGERLDAIVFTGGIGENSSLVRELTLNHLKLFGYQVDSDK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCC
NKAARFGHEGVITADNTPVAMVIPTNEELVIAQDTARLCIA
CHHHHCCCCCEEEECCCCEEEEEECCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100