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The map label for this gene is purH

Identifier: 15602087

GI number: 15602087

Start: 246443

End: 248044

Strand: Direct

Name: purH

Synonym: PM0222

Alternate gene names: 15602087

Gene position: 246443-248044 (Clockwise)

Preceding gene: 15602086

Following gene: 15602088

Centisome position: 10.92

GC content: 43.51

Gene sequence:

>1602_bases
ATGCAACCTAATCGTCCTATTCGCCAAGCATTACTGAGTGTTTCTGATAAAACAGGTATTGTTGAGTTCGCTCAAGCTCT
TGTTCAACGTGGTGTGAAATTACTTTCAACAGGTGGTACTGCCAAATTATTAGCTGACCACGGCTTAGCGGTAACAGAAG
TCTCAGATTACACAGGCTTTCCAGAAATGATGGATGGACGCGTGAAGACATTACATCCCAAAGTACATGGTGGTATTTTA
GGGCGTCGTGGTACAGATGATGAGGTGATGAGCCAACAGGGTATCGAAGGTATCGATATGGTGGTGGTGAATTTATACCC
TTTTGCTGCAACGGTTGCGAAACCAAATTGCAGTTTAGAAGAGGCAGTGGAAAATATTGATATTGGCGGACCAACAATGG
TGCGCTCAGCCGCGAAAAATCATCAAGATGTTGCAATTGTCGTTAATAATAGCGATTTCAATGCGATTCTGGCTGAAATG
GATCAACACCAAAATAGCCTAACGTTGGAAACTCGCTTTGATCTTGCGATTAAAGCGTTTGAACATACTGCACAATATGA
TGCCATGATTGCGAACTATTTTGGTCAATTAGTCAAACCTTATTTTGTTGCAGAGGAAGAAGATGCCGAGGCGAAGTGCG
GTCAGTTTCCACGTACTTTAAATTTAAACTTTATCCGTAAACAAACCATGCGTTATGGTGAGAATGGGCATCAAAAAGCC
GCTTTTTATGTGGAGCAAGACGTAAAAGAAGCTTCTGTATCCACCGCAAAACAATTACAAGGTAAAGCCCTTTCTTATAA
CAATATCGCTGATACCGATGCTGCGCTTGAATGTGTGAAAAGCTTTGATGAGCCGGCTTGTGTCATTGTGAAACATGCTA
ATCCTTGTGGTGTGGCATTAGGCGCAGACATTTTAGCGGCTTATAACCGGGCTTATCAGACTGACCCGACTTCCGCTTTC
GGTGGCATTATTGCCTTTAACCGTGAGTTAGATGCCAAAACCGCGCAAACGATTATTGATCGTCAATTCGTGGAAGTGAT
TATTGCTCCAACAGTTGCTGAAGAGGCAAAAGCTTTATTGAAAGCAAAGAAAAATGTGCGAGTGTTAGAATGTGGTGAGT
GGTCAGGTACGCAACAACGCCTAGATGTAAAGCGTGTCAATGGCGGTTTATTGGTTCAAGAAGCTGATTTAGGCATGGTC
GATTTAGCCGACTTAAAAGTGGTGAGCAAACGTCAGCCGACAGAGCAAGAATTAAAAGATTTGTTATTCTGCTGGAAAGT
GGCGAAATTTGTTAAATCGAATGCCATCGTGTATGCCAAAGATAACCAAACTATTGGTATTGGTGCGGGGCAAATGAGCC
GTGTCTATTCGGCTAAAATTGCGGGCATTAAAGCGCAAGATGAAGGACTGGATGTGGCAGGTTGTGTGATGGCGTCTGAT
GCGTTCTTCCCATTCCGTGATGGAATTGATGCTGCTGCCAAAGTAGGGATTCAATGCGTGATTCATCCAGGTGGTTCAAT
GCGTGATCAAGAAGTAATCGACGCCGCCGATGAACATAATATGGTGATGGTATTGACCGGAATGCGTCACTTTAGACACT
AG

Upstream 100 bases:

>100_bases
GTTTTTTTATTGAAAATCGTTACACAAGCGCAAACGTTTGCGTTATAATGCGACCTTTCTTTTATGACTTACTTTCAACG
CTTAATTTTAAGGATTTTCC

Downstream 100 bases:

>100_bases
GATGTGCAATGTCATTTTTGACATTAAGGTAAATCAAGATGAAAAAAAGTCTTTTTATTTTATTGGGCTATTCCGCCGTT
TTGCTATTGAGCGAAATACT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MQPNRPIRQALLSVSDKTGIVEFAQALVQRGVKLLSTGGTAKLLADHGLAVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGTDDEVMSQQGIEGIDMVVVNLYPFAATVAKPNCSLEEAVENIDIGGPTMVRSAAKNHQDVAIVVNNSDFNAILAEM
DQHQNSLTLETRFDLAIKAFEHTAQYDAMIANYFGQLVKPYFVAEEEDAEAKCGQFPRTLNLNFIRKQTMRYGENGHQKA
AFYVEQDVKEASVSTAKQLQGKALSYNNIADTDAALECVKSFDEPACVIVKHANPCGVALGADILAAYNRAYQTDPTSAF
GGIIAFNRELDAKTAQTIIDRQFVEVIIAPTVAEEAKALLKAKKNVRVLECGEWSGTQQRLDVKRVNGGLLVQEADLGMV
DLADLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLDVAGCVMASD
AFFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Sequences:

>Translated_533_residues
MQPNRPIRQALLSVSDKTGIVEFAQALVQRGVKLLSTGGTAKLLADHGLAVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGTDDEVMSQQGIEGIDMVVVNLYPFAATVAKPNCSLEEAVENIDIGGPTMVRSAAKNHQDVAIVVNNSDFNAILAEM
DQHQNSLTLETRFDLAIKAFEHTAQYDAMIANYFGQLVKPYFVAEEEDAEAKCGQFPRTLNLNFIRKQTMRYGENGHQKA
AFYVEQDVKEASVSTAKQLQGKALSYNNIADTDAALECVKSFDEPACVIVKHANPCGVALGADILAAYNRAYQTDPTSAF
GGIIAFNRELDAKTAQTIIDRQFVEVIIAPTVAEEAKALLKAKKNVRVLECGEWSGTQQRLDVKRVNGGLLVQEADLGMV
DLADLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLDVAGCVMASD
AFFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
>Mature_533_residues
MQPNRPIRQALLSVSDKTGIVEFAQALVQRGVKLLSTGGTAKLLADHGLAVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGTDDEVMSQQGIEGIDMVVVNLYPFAATVAKPNCSLEEAVENIDIGGPTMVRSAAKNHQDVAIVVNNSDFNAILAEM
DQHQNSLTLETRFDLAIKAFEHTAQYDAMIANYFGQLVKPYFVAEEEDAEAKCGQFPRTLNLNFIRKQTMRYGENGHQKA
AFYVEQDVKEASVSTAKQLQGKALSYNNIADTDAALECVKSFDEPACVIVKHANPCGVALGADILAAYNRAYQTDPTSAF
GGIIAFNRELDAKTAQTIIDRQFVEVIIAPTVAEEAKALLKAKKNVRVLECGEWSGTQQRLDVKRVNGGLLVQEADLGMV
DLADLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLDVAGCVMASD
AFFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=491, Percent_Identity=39.918533604888, Blast_Score=285, Evalue=6e-77,
Organism=Escherichia coli, GI1790439, Length=533, Percent_Identity=76.172607879925, Blast_Score=836, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=521, Percent_Identity=34.9328214971209, Blast_Score=248, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI71985574, Length=352, Percent_Identity=28.125, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=60, Percent_Identity=51.6666666666667, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=35.9756097560976, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=35.6854838709677, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24649832, Length=495, Percent_Identity=38.1818181818182, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PASMU (P57828)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245159.1
- ProteinModelPortal:   P57828
- SMR:   P57828
- GeneID:   1243569
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0222
- NMPDR:   fig|272843.1.peg.222
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- ProtClustDB:   PRK00881
- BioCyc:   PMUL272843:PM0222-MONOMER
- BRENDA:   2.1.2.3
- BRENDA:   3.5.4.10
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 58085; Mature: 58085

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPNRPIRQALLSVSDKTGIVEFAQALVQRGVKLLSTGGTAKLLADHGLAVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCEEEHHHCCEEEEEECCCCCC
PEMMDGRVKTLHPKVHGGILGRRGTDDEVMSQQGIEGIDMVVVNLYPFAATVAKPNCSLE
HHHHCCCCEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHEECCCCCCHH
EAVENIDIGGPTMVRSAAKNHQDVAIVVNNSDFNAILAEMDQHQNSLTLETRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EHTAQYDAMIANYFGQLVKPYFVAEEEDAEAKCGQFPRTLNLNFIRKQTMRYGENGHQKA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCCCEECHHHHHHHHHHHCCCCCCEE
AFYVEQDVKEASVSTAKQLQGKALSYNNIADTDAALECVKSFDEPACVIVKHANPCGVAL
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHH
GADILAAYNRAYQTDPTSAFGGIIAFNRELDAKTAQTIIDRQFVEVIIAPTVAEEAKALL
HHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAKKNVRVLECGEWSGTQQRLDVKRVNGGLLVQEADLGMVDLADLKVVSKRQPTEQELKD
HHHCCCEEEECCCCCCCHHHCCCEECCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHH
LLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLDVAGCVMASD
HHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCCCCEEHHEEECC
AFFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MQPNRPIRQALLSVSDKTGIVEFAQALVQRGVKLLSTGGTAKLLADHGLAVTEVSDYTGF
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCEEEHHHCCEEEEEECCCCCC
PEMMDGRVKTLHPKVHGGILGRRGTDDEVMSQQGIEGIDMVVVNLYPFAATVAKPNCSLE
HHHHCCCCEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHEECCCCCCHH
EAVENIDIGGPTMVRSAAKNHQDVAIVVNNSDFNAILAEMDQHQNSLTLETRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEEEHHHHHHHH
EHTAQYDAMIANYFGQLVKPYFVAEEEDAEAKCGQFPRTLNLNFIRKQTMRYGENGHQKA
HHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCCCCCEECHHHHHHHHHHHCCCCCCEE
AFYVEQDVKEASVSTAKQLQGKALSYNNIADTDAALECVKSFDEPACVIVKHANPCGVAL
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHH
GADILAAYNRAYQTDPTSAFGGIIAFNRELDAKTAQTIIDRQFVEVIIAPTVAEEAKALL
HHHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KAKKNVRVLECGEWSGTQQRLDVKRVNGGLLVQEADLGMVDLADLKVVSKRQPTEQELKD
HHHCCCEEEECCCCCCCHHHCCCEECCCCEEEEECCCCEEEHHHHHHHHCCCCCHHHHHH
LLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLDVAGCVMASD
HHHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCEECCCCCCEEHHEEECC
AFFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100