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The map label for this gene is menD

Identifier: 15601919

GI number: 15601919

Start: 59596

End: 61302

Strand: Direct

Name: menD

Synonym: PM0054

Alternate gene names: 15601919

Gene position: 59596-61302 (Clockwise)

Preceding gene: 15601918

Following gene: 15601920

Centisome position: 2.64

GC content: 42.41

Gene sequence:

>1707_bases
ATGTCAATAAGCGTATTTAATCGTTGTTGGTCAAAAGTGATTTTGGAAACCTTGGTTCGCCAAGGTGTTGCGCATTTTTG
TATTGCACCAGGATCTCGTTCTACTCCTCTGACTTTGGAAGCGATACGTTTGCAAGATAGTCATCGTGTGACTTGCCACA
CTCACTTTGATGAACGTGGACTTGGTTTTTTTGCGTTAGGATTGGCAAAAGTCAGCAAAAAGCCTGTTGCCGTGATCGTC
ACCTCTGGCACGGCAGCGGCGAATCTATATCCTGCTATTATTGAAGCGCGTCAAACGGGAGTAAATCTGATTGTTCTTAC
TGCTGATCGTCCTCCAGAATTGATCGAATGCGGGGCAAATCAAGCGATTGTACAACCGCATATGTATGCGGATTATCCTG
TTGCAAGTGTTAATTTACCGCGCCCAAGTCAAGATTACAGCGCCGATTGGTTAATTTCTCGTTTAGAGCAAGCCTGTTAT
CAACAAGCCCGTCAACCCGGTGTTGTTCATGTTAATGTGCCTTTTGCTGAACCCCTTTATGAAGCCAGTGAAACAGATAT
CGAGGCACATAGTTGGCTAGTGCCTATTCAACGTTGGTTAAACCAACATAAACCTTGGATACAACAACAAACAGATCAGA
AAGAAGTTTTGATGCACGCCAATTGGGATCATTGGCGGACGATGCGAGGAGTCATTGTTGTTGGGCGATTACCCGTGGAA
CAAACTATGGGCATTATGGCTTGGGCAAATACCATGGGCTGGATTGCTTTAACAGATGTTCAATCGAGTGTTGAAGCCAG
TTTACCTTATGCAGATATTTGGTTAGCGAATCAAACAGTTAAGCAAAAATTATTACAAGCGGATATTGTTATTCAATTTG
GCTCTGGGTTTATTAGTAAACGTGTGAATCAATTTTTAGCAGCCTTTAAACAAGAATATTGGATTGTTGAACAAAGTACG
GGCTTAATCGACCCAAATCATCATGCACATACACGCTTTAATGCGAAAGTGCATCATTGGTTACGTGCACATCCGCCATT
GCGCCAAAAACCGTGGTTACTTGAACCCCTTGCGCTGTCTAAATTCTGCGCTAGTTTTATTGAGAAACAAGTCGGTGGTA
ATTTAAATGAGGCATCATTAGCTCACCATTTAGACCGTATTTTATCCAACAACGGCGTTTTATTTTTAGGCAACAGTTTA
TTTGTTCGTTTAGTCGATGCGTTAGCGAAATTACCAGAAGGTTATCCTGTTTATACGAACCGTGGAGCAAGTGGTATTGA
TGGTCTTTTAGCCACCGCCGCAGGCATTGGGATTGGTGCAAATCAACCTTTAGTCGCGATGATCGGGGACGTGTCTGCCC
TTTACGACATTAATTCCCTTGCGTTGTTTAAAAAAGTCACTCAACCGACTATCATTTTTGTGATCAATAATAATGGTGGT
GCGATTTTTGATATGTTGCCTGTTGAGGCGGATGTGAAAGAAAAGTATTACCGCATGCCACATCATTTAGAGTTTTCACA
GCTCGTGCAAACCTTTGATTTAAAATATGCACGTCCTTATACTTGGGCAGATTTAGGCGCGGTATTAAAAGTTGCCTATT
CACGCCGTGAAACCACATTAATTGAGATTAAAGTAGGGGCGAGTGATGCCAGTACGATTTATAAGCGCCTGATTGAGCAA
ATTAGCTATGCGGTGATCGGTGAATAA

Upstream 100 bases:

>100_bases
TGAAGGCTCTATTCCATTGTTAGAATGGCGAGAGATTGAGCGTAAAGCTATGGGCTTAGTGTCGTTATTGCAACAGAATC
AATTATAAAAGGGAACAGAT

Downstream 100 bases:

>100_bases
AAATAAATGCTCGCTTGGCGGGCATTTTTTAAGGAGTCAGAATGCCTGTTTTTGTTTTTTTACATGGCTTATTAGGGAGC
AAGCACGATTGGCAAAAAGT

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSISVFNRCWSKVILETLVRQGVAHFCIAPGSRSTPLTLEAIRLQDSHRVTCHTHFDERGLGFFALGLAKVSKKPVAVIV
TSGTAAANLYPAIIEARQTGVNLIVLTADRPPELIECGANQAIVQPHMYADYPVASVNLPRPSQDYSADWLISRLEQACY
QQARQPGVVHVNVPFAEPLYEASETDIEAHSWLVPIQRWLNQHKPWIQQQTDQKEVLMHANWDHWRTMRGVIVVGRLPVE
QTMGIMAWANTMGWIALTDVQSSVEASLPYADIWLANQTVKQKLLQADIVIQFGSGFISKRVNQFLAAFKQEYWIVEQST
GLIDPNHHAHTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCASFIEKQVGGNLNEASLAHHLDRILSNNGVLFLGNSL
FVRLVDALAKLPEGYPVYTNRGASGIDGLLATAAGIGIGANQPLVAMIGDVSALYDINSLALFKKVTQPTIIFVINNNGG
AIFDMLPVEADVKEKYYRMPHHLEFSQLVQTFDLKYARPYTWADLGAVLKVAYSRRETTLIEIKVGASDASTIYKRLIEQ
ISYAVIGE

Sequences:

>Translated_568_residues
MSISVFNRCWSKVILETLVRQGVAHFCIAPGSRSTPLTLEAIRLQDSHRVTCHTHFDERGLGFFALGLAKVSKKPVAVIV
TSGTAAANLYPAIIEARQTGVNLIVLTADRPPELIECGANQAIVQPHMYADYPVASVNLPRPSQDYSADWLISRLEQACY
QQARQPGVVHVNVPFAEPLYEASETDIEAHSWLVPIQRWLNQHKPWIQQQTDQKEVLMHANWDHWRTMRGVIVVGRLPVE
QTMGIMAWANTMGWIALTDVQSSVEASLPYADIWLANQTVKQKLLQADIVIQFGSGFISKRVNQFLAAFKQEYWIVEQST
GLIDPNHHAHTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCASFIEKQVGGNLNEASLAHHLDRILSNNGVLFLGNSL
FVRLVDALAKLPEGYPVYTNRGASGIDGLLATAAGIGIGANQPLVAMIGDVSALYDINSLALFKKVTQPTIIFVINNNGG
AIFDMLPVEADVKEKYYRMPHHLEFSQLVQTFDLKYARPYTWADLGAVLKVAYSRRETTLIEIKVGASDASTIYKRLIEQ
ISYAVIGE
>Mature_567_residues
SISVFNRCWSKVILETLVRQGVAHFCIAPGSRSTPLTLEAIRLQDSHRVTCHTHFDERGLGFFALGLAKVSKKPVAVIVT
SGTAAANLYPAIIEARQTGVNLIVLTADRPPELIECGANQAIVQPHMYADYPVASVNLPRPSQDYSADWLISRLEQACYQ
QARQPGVVHVNVPFAEPLYEASETDIEAHSWLVPIQRWLNQHKPWIQQQTDQKEVLMHANWDHWRTMRGVIVVGRLPVEQ
TMGIMAWANTMGWIALTDVQSSVEASLPYADIWLANQTVKQKLLQADIVIQFGSGFISKRVNQFLAAFKQEYWIVEQSTG
LIDPNHHAHTRFNAKVHHWLRAHPPLRQKPWLLEPLALSKFCASFIEKQVGGNLNEASLAHHLDRILSNNGVLFLGNSLF
VRLVDALAKLPEGYPVYTNRGASGIDGLLATAAGIGIGANQPLVAMIGDVSALYDINSLALFKKVTQPTIIFVINNNGGA
IFDMLPVEADVKEKYYRMPHHLEFSQLVQTFDLKYARPYTWADLGAVLKVAYSRRETTLIEIKVGASDASTIYKRLIEQI
SYAVIGE

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=565, Percent_Identity=48.4955752212389, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PASMU (Q9CPI4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_244991.1
- ProteinModelPortal:   Q9CPI4
- GeneID:   1243401
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0054
- NMPDR:   fig|272843.1.peg.54
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   PMUL272843:PM0054-MONOMER
- BRENDA:   2.5.1.64
- BRENDA:   4.1.1.71
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63378; Mature: 63246

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISVFNRCWSKVILETLVRQGVAHFCIAPGSRSTPLTLEAIRLQDSHRVTCHTHFDERG
CCCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LGFFALGLAKVSKKPVAVIVTSGTAAANLYPAIIEARQTGVNLIVLTADRPPELIECGAN
CCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCC
QAIVQPHMYADYPVASVNLPRPSQDYSADWLISRLEQACYQQARQPGVVHVNVPFAEPLY
CEEECCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
EASETDIEAHSWLVPIQRWLNQHKPWIQQQTDQKEVLMHANWDHWRTMRGVIVVGRLPVE
CCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHEEEECCCHHHHHCCCEEEEECCCHH
QTMGIMAWANTMGWIALTDVQSSVEASLPYADIWLANQTVKQKLLQADIVIQFGSGFISK
HHHHHHHHHCCCCEEEEECCHHHHHHCCCCCEEEECCHHHHHHHHHHHEEEEECCCHHHH
RVNQFLAAFKQEYWIVEQSTGLIDPNHHAHTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCASFIEKQVGGNLNEASLAHHLDRILSNNGVLFLGNSLFVRLVDALAKLPEGYPVYTN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEC
RGASGIDGLLATAAGIGIGANQPLVAMIGDVSALYDINSLALFKKVTQPTIIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AIFDMLPVEADVKEKYYRMPHHLEFSQLVQTFDLKYARPYTWADLGAVLKVAYSRRETTL
EEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IEIKVGASDASTIYKRLIEQISYAVIGE
EEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SISVFNRCWSKVILETLVRQGVAHFCIAPGSRSTPLTLEAIRLQDSHRVTCHTHFDERG
CCHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCCC
LGFFALGLAKVSKKPVAVIVTSGTAAANLYPAIIEARQTGVNLIVLTADRPPELIECGAN
CCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCC
QAIVQPHMYADYPVASVNLPRPSQDYSADWLISRLEQACYQQARQPGVVHVNVPFAEPLY
CEEECCCEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHH
EASETDIEAHSWLVPIQRWLNQHKPWIQQQTDQKEVLMHANWDHWRTMRGVIVVGRLPVE
CCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCHHEEEECCCHHHHHCCCEEEEECCCHH
QTMGIMAWANTMGWIALTDVQSSVEASLPYADIWLANQTVKQKLLQADIVIQFGSGFISK
HHHHHHHHHCCCCEEEEECCHHHHHHCCCCCEEEECCHHHHHHHHHHHEEEEECCCHHHH
RVNQFLAAFKQEYWIVEQSTGLIDPNHHAHTRFNAKVHHWLRAHPPLRQKPWLLEPLALS
HHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCCCCCHHHHH
KFCASFIEKQVGGNLNEASLAHHLDRILSNNGVLFLGNSLFVRLVDALAKLPEGYPVYTN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEC
RGASGIDGLLATAAGIGIGANQPLVAMIGDVSALYDINSLALFKKVTQPTIIFVINNNGG
CCCCCHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
AIFDMLPVEADVKEKYYRMPHHLEFSQLVQTFDLKYARPYTWADLGAVLKVAYSRRETTL
EEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEE
IEIKVGASDASTIYKRLIEQISYAVIGE
EEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100