Click here to switch to the map view.

The map label for this gene is uvrB

Identifier: 15595181

GI number: 15595181

Start: 888297

End: 890318

Strand: Direct

Name: uvrB

Synonym: BB0836

Alternate gene names: 15595181

Gene position: 888297-890318 (Clockwise)

Preceding gene: 15595176

Following gene: 15595182

Centisome position: 97.54

GC content: 30.97

Gene sequence:

>2022_bases
TTGGTCGTTAAATTAATGATAGATTTTTTTTTGAAGTCAGAATATCTTCCTGCTGGGGATCAGCCTAAGGCAATAAAAGA
GATTGAAAATTCTATTTTGCTTGGAAATAAATATCAAACTTTAAAAGGTGTTACAGGCAGTGGAAAGACTTTTACAATTG
CAAATATAATAAAAGATCTAAACAGGCCTGCCTTAGTTGTCAGTCACAACAAAACATTAGCAGCACAGCTTTATAGAGAG
TTTAAAGATTTTTTTCCAAATAATGCTGTTGAGTATTTTGTTTCTTATTATGATTATTATCAGCCAGAATCTTATGTTCC
TTCAAAAGATTTATTTATTGAAAAAGAAGCCACTATTAATACTGAGATAGAAATTAAGCGAATAAGAACGGTAACGTCTC
TTGCTAAAAGACGAGATGTAATTGTTGTTGCAACCGTATCTTCAATTTATGCTCTTGGATCTCCAGATTTTTTCAAAAAA
TCAGCACGAGAATTTTTTGTAGGTCAAAAGATTTCTATTAAAGAAATATCAGATATTTTTGTAGAGCTTTATTATGAGAG
AACTTTAATGAATCTAGAAAGAGATAAATTTTCGATTAAGGGAGATATTGTTGAAATTTGGCCTAGCAGTGAACATGGAG
AGTTTGCTTATCGAATTTGTTTAGATTTTGATGAAATTGTTGAAATATATAGAGTTAGTTCATTTTCTAAAAAAAATTTA
GGAGCTACAAATAGTTTTACTCTTTTTGCTAAATCTTATTTTGTAATTCCTTATGAAAATGTATTAGAAGCGATACCCAA
AATATCTCATGATTTAAGTCTTCAATGTCAATATTTTAAAGACAATGGCAAACTTGTAGAAGCCGAGAGACTCAAACAGA
GGGTAGAGTATGATTTGGAAATGCTTAGAGAAACAGGGTTTTGTTCGGGCATTGAAAATTATTCTAAATATTTGAGTGGA
AGTACAATGGAAAGACCTTATTGTCTTTTTGATTTTTTTCCGAAAGATWACTTATTATTTGTAGATGAATCTCATGTTAC
ATTGCCTCAATTTAGGGGAATGTATAATGGAGATCATTCTAGAAAATTAAATCTTGTTAACTTTGGGTTTAGACTTCCTG
CAGCGCTTGAAAACAGACCTCTTAAATATGATGAATTTGAAGCATTAATTAATCAGGTTGTGTTTGTATCTGCAACCCCG
GGTGTTGAAGAGAATGAGAAAAGTAGTGTGGTTGTTGACCAAATAATTCGTCCAACAGGTCTTGTTGATCCTGAAATTAT
TACTAGGCACTCTGATGGGCAAATGGAAGATCTTTACAGCGAAATTCAAAAAAGAGTGGCTCTTAAAGAGCGGGTTTTAA
TTACTACTTTGACAAAAAAAATGTCTGAGGATTTGACTGAATATTTGGTAAATCTTGGCGTAAGGGCAAAATATTTACAT
TCAGAGCTTGATACCCTTGAGAGAGTGGAAGTTATTTCGTTGCTTAGAAAATCTGAAATCGATGTTATTGTTGGGATTAA
CCTGCTTAGAGAGGGCTTAGATATTCCAGAAGTATCTCTTGTTGCAATATTAGATGCTGATAAGGTGGGATTTTTAAGAT
CTACTACTTCATTAATACAAACAATTGGTAGGGCTGCTAGAAATTCTAATGGACTTGTAATAATGTATTACGACAAAATT
AGTTTAGCTATGCGGGAGGCAATTGAGGAGACTAATAGAAGACGTCAAATTCAGATTGATTATAATAAAAAAAATAATAT
TACTCCTAAGACAATTGTTAAAAAGATTCAAAATATTTTAGAAAAGGAACTTAATAATAAAAATAAAAATGTTGGCTATG
ATTTTGAAAAAATTATTTCGGGTGAGAGATTGTCTAAAAAAAAGCTTATTGATAAGCTTAAATTTGACTTAGAAGAAGCT
GTTAATGATGAAAGATTTGAAGACGCAATTGTTTTAAGAGATAAAATAAAAGAGCTTAGTAGCAAAATTAGTATAGCTCG
TAATAAAAAAAGAGAGGTGTAA

Upstream 100 bases:

>100_bases
TTATTAAACAAAGATTAATTAAATTATATTGATTTATAATGAATTATATACTTGATATAGAATTTATTATCATATAATTT
ATAAGAATGTGTATTAAATT

Downstream 100 bases:

>100_bases
TTTTTGGAAAAAAGTTTGAAAAAAAAAATTATTGTCAGAGGAGCAAAAGAACATAATTTAAAAAATGTTGATGTAGATAT
TCCAAAAGATGGTTTAGTTG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MVVKLMIDFFLKSEYLPAGDQPKAIKEIENSILLGNKYQTLKGVTGSGKTFTIANIIKDLNRPALVVSHNKTLAAQLYRE
FKDFFPNNAVEYFVSYYDYYQPESYVPSKDLFIEKEATINTEIEIKRIRTVTSLAKRRDVIVVATVSSIYALGSPDFFKK
SAREFFVGQKISIKEISDIFVELYYERTLMNLERDKFSIKGDIVEIWPSSEHGEFAYRICLDFDEIVEIYRVSSFSKKNL
GATNSFTLFAKSYFVIPYENVLEAIPKISHDLSLQCQYFKDNGKLVEAERLKQRVEYDLEMLRETGFCSGIENYSKYLSG
STMERPYCLFDFFPKDXLLFVDESHVTLPQFRGMYNGDHSRKLNLVNFGFRLPAALENRPLKYDEFEALINQVVFVSATP
GVEENEKSSVVVDQIIRPTGLVDPEIITRHSDGQMEDLYSEIQKRVALKERVLITTLTKKMSEDLTEYLVNLGVRAKYLH
SELDTLERVEVISLLRKSEIDVIVGINLLREGLDIPEVSLVAILDADKVGFLRSTTSLIQTIGRAARNSNGLVIMYYDKI
SLAMREAIEETNRRRQIQIDYNKKNNITPKTIVKKIQNILEKELNNKNKNVGYDFEKIISGERLSKKKLIDKLKFDLEEA
VNDERFEDAIVLRDKIKELSSKISIARNKKREV

Sequences:

>Translated_673_residues
MVVKLMIDFFLKSEYLPAGDQPKAIKEIENSILLGNKYQTLKGVTGSGKTFTIANIIKDLNRPALVVSHNKTLAAQLYRE
FKDFFPNNAVEYFVSYYDYYQPESYVPSKDLFIEKEATINTEIEIKRIRTVTSLAKRRDVIVVATVSSIYALGSPDFFKK
SAREFFVGQKISIKEISDIFVELYYERTLMNLERDKFSIKGDIVEIWPSSEHGEFAYRICLDFDEIVEIYRVSSFSKKNL
GATNSFTLFAKSYFVIPYENVLEAIPKISHDLSLQCQYFKDNGKLVEAERLKQRVEYDLEMLRETGFCSGIENYSKYLSG
STMERPYCLFDFFPKDXLLFVDESHVTLPQFRGMYNGDHSRKLNLVNFGFRLPAALENRPLKYDEFEALINQVVFVSATP
GVEENEKSSVVVDQIIRPTGLVDPEIITRHSDGQMEDLYSEIQKRVALKERVLITTLTKKMSEDLTEYLVNLGVRAKYLH
SELDTLERVEVISLLRKSEIDVIVGINLLREGLDIPEVSLVAILDADKVGFLRSTTSLIQTIGRAARNSNGLVIMYYDKI
SLAMREAIEETNRRRQIQIDYNKKNNITPKTIVKKIQNILEKELNNKNKNVGYDFEKIISGERLSKKKLIDKLKFDLEEA
VNDERFEDAIVLRDKIKELSSKISIARNKKREV
>Mature_673_residues
MVVKLMIDFFLKSEYLPAGDQPKAIKEIENSILLGNKYQTLKGVTGSGKTFTIANIIKDLNRPALVVSHNKTLAAQLYRE
FKDFFPNNAVEYFVSYYDYYQPESYVPSKDLFIEKEATINTEIEIKRIRTVTSLAKRRDVIVVATVSSIYALGSPDFFKK
SAREFFVGQKISIKEISDIFVELYYERTLMNLERDKFSIKGDIVEIWPSSEHGEFAYRICLDFDEIVEIYRVSSFSKKNL
GATNSFTLFAKSYFVIPYENVLEAIPKISHDLSLQCQYFKDNGKLVEAERLKQRVEYDLEMLRETGFCSGIENYSKYLSG
STMERPYCLFDFFPKDXLLFVDESHVTLPQFRGMYNGDHSRKLNLVNFGFRLPAALENRPLKYDEFEALINQVVFVSATP
GVEENEKSSVVVDQIIRPTGLVDPEIITRHSDGQMEDLYSEIQKRVALKERVLITTLTKKMSEDLTEYLVNLGVRAKYLH
SELDTLERVEVISLLRKSEIDVIVGINLLREGLDIPEVSLVAILDADKVGFLRSTTSLIQTIGRAARNSNGLVIMYYDKI
SLAMREAIEETNRRRQIQIDYNKKNNITPKTIVKKIQNILEKELNNKNKNVGYDFEKIISGERLSKKKLIDKLKFDLEEA
VNDERFEDAIVLRDKIKELSSKISIARNKKREV

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=671, Percent_Identity=51.2667660208644, Blast_Score=635, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_BORBU (O51776)

Other databases:

- EMBL:   AE000783
- PIR:   C70204
- RefSeq:   NP_212970.1
- EnsemblBacteria:   EBBORT00000008375
- GeneID:   1195694
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0836
- NMPDR:   fig|224326.1.peg.1220
- TIGR:   BB_0836
- GeneTree:   EBGT00050000006756
- HOGENOM:   HBG703949
- OMA:   EKDLAIN
- ProtClustDB:   PRK05298
- BioCyc:   BBUR224326:BB_0836-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR

EC number: NA

Molecular weight: Translated: 77512; Mature: 77512

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKLMIDFFLKSEYLPAGDQPKAIKEIENSILLGNKYQTLKGVTGSGKTFTIANIIKDL
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCEEECCCCHHHCCCCCCCCEEEHHHHHHHC
NRPALVVSHNKTLAAQLYREFKDFFPNNAVEYFVSYYDYYQPESYVPSKDLFIEKEATIN
CCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
TEIEIKRIRTVTSLAKRRDVIVVATVSSIYALGSPDFFKKSAREFFVGQKISIKEISDIF
CCEEHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VELYYERTLMNLERDKFSIKGDIVEIWPSSEHGEFAYRICLDFDEIVEIYRVSSFSKKNL
HHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
GATNSFTLFAKSYFVIPYENVLEAIPKISHDLSLQCQYFKDNGKLVEAERLKQRVEYDLE
CCCCCEEEEEEEEEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHH
MLRETGFCSGIENYSKYLSGSTMERPYCLFDFFPKDXLLFVDESHVTLPQFRGMYNGDHS
HHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCC
RKLNLVNFGFRLPAALENRPLKYDEFEALINQVVFVSATPGVEENEKSSVVVDQIIRPTG
CEEEEEEECCCCCHHHCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHCCCC
LVDPEIITRHSDGQMEDLYSEIQKRVALKERVLITTLTKKMSEDLTEYLVNLGVRAKYLH
CCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SELDTLERVEVISLLRKSEIDVIVGINLLREGLDIPEVSLVAILDADKVGFLRSTTSLIQ
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
TIGRAARNSNGLVIMYYDKISLAMREAIEETNRRRQIQIDYNKKNNITPKTIVKKIQNIL
HHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
EKELNNKNKNVGYDFEKIISGERLSKKKLIDKLKFDLEEAVNDERFEDAIVLRDKIKELS
HHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SKISIARNKKREV
HHHHHHHHHCCCC
>Mature Secondary Structure
MVVKLMIDFFLKSEYLPAGDQPKAIKEIENSILLGNKYQTLKGVTGSGKTFTIANIIKDL
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCEEECCCCHHHCCCCCCCCEEEHHHHHHHC
NRPALVVSHNKTLAAQLYREFKDFFPNNAVEYFVSYYDYYQPESYVPSKDLFIEKEATIN
CCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCC
TEIEIKRIRTVTSLAKRRDVIVVATVSSIYALGSPDFFKKSAREFFVGQKISIKEISDIF
CCEEHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
VELYYERTLMNLERDKFSIKGDIVEIWPSSEHGEFAYRICLDFDEIVEIYRVSSFSKKNL
HHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
GATNSFTLFAKSYFVIPYENVLEAIPKISHDLSLQCQYFKDNGKLVEAERLKQRVEYDLE
CCCCCEEEEEEEEEEEEHHHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHH
MLRETGFCSGIENYSKYLSGSTMERPYCLFDFFPKDXLLFVDESHVTLPQFRGMYNGDHS
HHHHCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCCCHHHCCCCCCCCC
RKLNLVNFGFRLPAALENRPLKYDEFEALINQVVFVSATPGVEENEKSSVVVDQIIRPTG
CEEEEEEECCCCCHHHCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHCCCC
LVDPEIITRHSDGQMEDLYSEIQKRVALKERVLITTLTKKMSEDLTEYLVNLGVRAKYLH
CCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SELDTLERVEVISLLRKSEIDVIVGINLLREGLDIPEVSLVAILDADKVGFLRSTTSLIQ
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHH
TIGRAARNSNGLVIMYYDKISLAMREAIEETNRRRQIQIDYNKKNNITPKTIVKKIQNIL
HHHHHHCCCCCEEEEEECHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
EKELNNKNKNVGYDFEKIISGERLSKKKLIDKLKFDLEEAVNDERFEDAIVLRDKIKELS
HHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SKISIARNKKREV
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685