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The map label for this gene is sbcD

Identifier: 15595174

GI number: 15595174

Start: 875207

End: 876448

Strand: Direct

Name: sbcD

Synonym: BB0829

Alternate gene names: 15595174

Gene position: 875207-876448 (Clockwise)

Preceding gene: 15595169

Following gene: 15595175

Centisome position: 96.1

GC content: 28.42

Gene sequence:

>1242_bases
GTGAGCAATTATAAAATTCTGCATACTTCAGATTGGCACATTGGAAAAAAAATTGAAAATTTTTCAATTCTTAAGGAGCA
GAAAAATTTTTTATATTTTCTTTTAGAATTTATTAAAAAAGAAAATATAGATCTTTTACTTGTTGCCGGGGATGTTTATG
ATTCCAAAAGGCCTGGGTTTGAAGAGCAAAGATTAGTGAATAATTTTTTTTATGAACTTTCTTTTACTTCTTGTAAATGG
TGTGTGGTTATTTCTGGAAATCATGACAAAAAGGATTATTTGAGTATAAATAAAAAACTTCTCTCACGGTTTAATTTTTT
TTTAATAACTGAATATGATTCTGATGAGCAAATAGTTTTATTAAAAGATAATGGAAATCTTAAGTTTATTGTTGTTTGTC
TTCCGCATATAAATGAAAGGCTTATTTTAGGTCAAAATTTTGACAATATTTTTGGACTGGAAGATCAGTATTCCAGCAAA
CTATTTCTTGAAAATTTAGAAAATGCCTACAGAGAAAAAATATCAAATTTATCTAATTTTTTAGAAAACAAGTATAAAGG
AATTCCTAAGATATTAATGGCGCATTCTTTTTTTGGCAGCAGTAAAAAGATTGACACCTTGGGAGGTAGTTATATTATCC
CTTTTAATGTTTTTGGAAATGGTTTTTCTTATGTTGCTCTTGGGCATATTCATAAGTTCATGAAACTAAGAGATAATATT
GTTTATTCAGGATCTCCTATGCAATATTCATTTAATGAGACCTGTGATAAATACATAAACGTTTTACATTTTAATGACAA
TAAATTAATCTTGCAAGAAGCATTTCCGGTGCCGATTTTCAATAAATTAATCTTTGCTAAAGGTTCTTTAAATGAAGTTC
TTGATTTTTTGGCTAATTCCAAAAAAGAAGAGTCTTTTACTATTTATTTGAAAATTGAACTTAATGAGGCAGTTGATACT
AGCGCTGAGGAGTCTATTTATGATTTAGCAAGGCTTAATTTTATGAATTTAGTTTCTATTTCCTATTCTTTACCCTCAAG
TCAGGATTTGCAAGACGATTCCAACTTTATTGGAGAACTTGAAGTGCTTGAAATGGATGAAAAATATTTTTTTGAGAAAA
AGTTGAGATGGGATTTTGAGAACGGTGTTATTAGGGATATCAAATTTAAGGAAGAAGAGCTTATTTCTCTTTTTAACGAG
GTTTTAGCTAACGGATATTTGGGTGAATATGAGGATAAATAA

Upstream 100 bases:

>100_bases
ACTATTATCAACTTTTTATTTTGCATAAATTCCCTATGTTTATATATTGTATTATAATATATAAATATAATTTAAGAATT
AAAAAAAGTGAGAGTATTTT

Downstream 100 bases:

>100_bases
GCTCATATTTAAAAATATTGCTTCTTATAAAGGCGAACATGAGTTGAATTTCGATACACTTCTTTTAAGACAATCGGGCA
TTTTTTTAATTTCTGGTAAT

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNYKILHTSDWHIGKKIENFSILKEQKNFLYFLLEFIKKENIDLLLVAGDVYDSKRPGFEEQRLVNNFFYELSFTSCKW
CVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVLLKDNGNLKFIVVCLPHINERLILGQNFDNIFGLEDQYSSK
LFLENLENAYREKISNLSNFLENKYKGIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLRDNI
VYSGSPMQYSFNETCDKYINVLHFNDNKLILQEAFPVPIFNKLIFAKGSLNEVLDFLANSKKEESFTIYLKIELNEAVDT
SAEESIYDLARLNFMNLVSISYSLPSSQDLQDDSNFIGELEVLEMDEKYFFEKKLRWDFENGVIRDIKFKEEELISLFNE
VLANGYLGEYEDK

Sequences:

>Translated_413_residues
MSNYKILHTSDWHIGKKIENFSILKEQKNFLYFLLEFIKKENIDLLLVAGDVYDSKRPGFEEQRLVNNFFYELSFTSCKW
CVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVLLKDNGNLKFIVVCLPHINERLILGQNFDNIFGLEDQYSSK
LFLENLENAYREKISNLSNFLENKYKGIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLRDNI
VYSGSPMQYSFNETCDKYINVLHFNDNKLILQEAFPVPIFNKLIFAKGSLNEVLDFLANSKKEESFTIYLKIELNEAVDT
SAEESIYDLARLNFMNLVSISYSLPSSQDLQDDSNFIGELEVLEMDEKYFFEKKLRWDFENGVIRDIKFKEEELISLFNE
VLANGYLGEYEDK
>Mature_412_residues
SNYKILHTSDWHIGKKIENFSILKEQKNFLYFLLEFIKKENIDLLLVAGDVYDSKRPGFEEQRLVNNFFYELSFTSCKWC
VVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVLLKDNGNLKFIVVCLPHINERLILGQNFDNIFGLEDQYSSKL
FLENLENAYREKISNLSNFLENKYKGIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLRDNIV
YSGSPMQYSFNETCDKYINVLHFNDNKLILQEAFPVPIFNKLIFAKGSLNEVLDFLANSKKEESFTIYLKIELNEAVDTS
AEESIYDLARLNFMNLVSISYSLPSSQDLQDDSNFIGELEVLEMDEKYFFEKKLRWDFENGVIRDIKFKEEELISLFNEV
LANGYLGEYEDK

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family

Homologues:

Organism=Escherichia coli, GI1786598, Length=350, Percent_Identity=27.1428571428571, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SBCD_BORBU (O51769)

Other databases:

- EMBL:   AE000783
- PIR:   D70203
- RefSeq:   NP_212963.1
- ProteinModelPortal:   O51769
- EnsemblBacteria:   EBBORT00000008363
- GeneID:   1195687
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0829
- NMPDR:   fig|224326.1.peg.1213
- TIGR:   BB_0829
- GeneTree:   EBGT00050000007571
- HOGENOM:   HBG352972
- OMA:   SDWHIGK
- PhylomeDB:   O51769
- ProtClustDB:   CLSK507868
- BioCyc:   BBUR224326:BB_0829-MONOMER
- InterPro:   IPR004843
- InterPro:   IPR004593
- TIGRFAMs:   TIGR00619

Pfam domain/function: PF00149 Metallophos

EC number: NA

Molecular weight: Translated: 48241; Mature: 48109

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYKILHTSDWHIGKKIENFSILKEQKNFLYFLLEFIKKENIDLLLVAGDVYDSKRPGF
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
EEQRLVNNFFYELSFTSCKWCVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVL
HHHHHHHHHHEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEE
LKDNGNLKFIVVCLPHINERLILGQNFDNIFGLEDQYSSKLFLENLENAYREKISNLSNF
EECCCCEEEEEEEECCCCCEEEEECCCHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHH
LENKYKGIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLRDNI
HHHHHCCCHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCE
VYSGSPMQYSFNETCDKYINVLHFNDNKLILQEAFPVPIFNKLIFAKGSLNEVLDFLANS
EECCCCCEECHHHHHHHHEEEEEECCCEEEEEECCCCCHHHHHEECCCCHHHHHHHHHCC
KKEESFTIYLKIELNEAVDTSAEESIYDLARLNFMNLVSISYSLPSSQDLQDDSNFIGEL
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCHHEEEEEEECCCCCCCCCCCCHHHCEE
EVLEMDEKYFFEKKLRWDFENGVIRDIKFKEEELISLFNEVLANGYLGEYEDK
EEEECCHHHHHHHHHCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNYKILHTSDWHIGKKIENFSILKEQKNFLYFLLEFIKKENIDLLLVAGDVYDSKRPGF
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
EEQRLVNNFFYELSFTSCKWCVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVL
HHHHHHHHHHEEEEECCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEE
LKDNGNLKFIVVCLPHINERLILGQNFDNIFGLEDQYSSKLFLENLENAYREKISNLSNF
EECCCCEEEEEEEECCCCCEEEEECCCHHHCCCCHHHCHHHHHHHHHHHHHHHHHHHHHH
LENKYKGIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLRDNI
HHHHHCCCHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCE
VYSGSPMQYSFNETCDKYINVLHFNDNKLILQEAFPVPIFNKLIFAKGSLNEVLDFLANS
EECCCCCEECHHHHHHHHEEEEEECCCEEEEEECCCCCHHHHHEECCCCHHHHHHHHHCC
KKEESFTIYLKIELNEAVDTSAEESIYDLARLNFMNLVSISYSLPSSQDLQDDSNFIGEL
CCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHCHHEEEEEEECCCCCCCCCCCCHHHCEE
EVLEMDEKYFFEKKLRWDFENGVIRDIKFKEEELISLFNEVLANGYLGEYEDK
EEEECCHHHHHHHHHCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685