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The map label for this gene is miaA

Identifier: 15595166

GI number: 15595166

Start: 866681

End: 867601

Strand: Direct

Name: miaA

Synonym: BB0821

Alternate gene names: 15595166

Gene position: 866681-867601 (Clockwise)

Preceding gene: 15595165

Following gene: 15595167

Centisome position: 95.16

GC content: 27.04

Gene sequence:

>921_bases
TTGAAGGAAGATAGAGTAGTTTTTATTTTTGGCCCTACAGCTGTGGGCAAAAGCAATATTTTATTTCATTTTCCAAAAAA
TAAAGCAGAAATTATTAATGTTGACTCTATTCAAGTTTATAAAGAGTTTAATATAGCTTCTTCAAAGCCAAGTAAAAATT
TAATGAAACATATAAAGCATCATTTAGTAGATTTTTTAGATCCCGAAAAGGATTATACTATTGGAATTTTTTACGAACAA
GCTTTAAAAATAGTAAAAGAAATAAGACAGAAAAAAAAAATTCCTATATTTGTAGGAGGTACTGCTTTTTATTTTAAGCA
TTTAAAGGATGGATTTCCTTCAACACCCTTGGTTACTTCTAAAATAAGAATTTATGTAAACAATCTTTTAGAGCTTAAGG
GTAAATCTTATCTTTTAAAAGAATTAAAAAATGTAGATCCCATCAGATTTAATATGTTAAATAAGAATGATATTTATCGC
ATTAAAAGATCGCTTGAGGTTTACTATCAAACAGGAATTCCTATTAGCCAATTTCAAAAAAAACAAAGTAGCGAATTTAA
AAATATTGTGATTATAGGCCTTAAGAGATCTTTTGAAGATTTGAAAACCAGAATATCAATAAGAATTAATGAAATGCTTA
ATAGTGGACTACTTTCCGAGATTAAAGGTTTATTTAGCAAGGGTTACAATGAAAATACTCCGGCTTTTAAAGGAATAGGC
TATAATGAGTTTTTGTTATGGAAAAGTAGACCTTGCTATGGTTTAAATGATATAATAGGTTTAATAAACAAAAATTCGTT
TTTATATGCAAAAAGGCAAATGACCTTTTTTGCCAAAATTTCTGATGTTTTATGGCTTCATCCAGAGGATGATTTAGATA
ACATTTTGAATTTAATTTTTAAGGTTGATAAGGAGATTTAG

Upstream 100 bases:

>100_bases
ATTATAGACAATATTTCTTTAGCAGAACACGCTATTTTTGATGATAAAATATTAGGGCAAGCTTTTAATGACGTTTTGAC
TGGTAAATTTAGATATTCAA

Downstream 100 bases:

>100_bases
GGAGTGCCTTGCGGAAGAAAAAGAAAATTAAAAAAAATTTCTACCCATAAAAGGAAGAAAAAAAGAAGAAAAAATAGACA
TAAGAAAAAAAATAAGTAAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKEDRVVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKDYTIGIFYEQ
ALKIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSKIRIYVNNLLELKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFQKKQSSEFKNIVIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYGLNDIIGLINKNSFLYAKRQMTFFAKISDVLWLHPEDDLDNILNLIFKVDKEI

Sequences:

>Translated_306_residues
MKEDRVVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKDYTIGIFYEQ
ALKIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSKIRIYVNNLLELKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFQKKQSSEFKNIVIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYGLNDIIGLINKNSFLYAKRQMTFFAKISDVLWLHPEDDLDNILNLIFKVDKEI
>Mature_306_residues
MKEDRVVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKDYTIGIFYEQ
ALKIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSKIRIYVNNLLELKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFQKKQSSEFKNIVIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYGLNDIIGLINKNSFLYAKRQMTFFAKISDVLWLHPEDDLDNILNLIFKVDKEI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=269, Percent_Identity=30.4832713754647, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790613, Length=316, Percent_Identity=28.1645569620253, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25144712, Length=292, Percent_Identity=28.4246575342466, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=30.3448275862069, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BORBU (O51761)

Other databases:

- EMBL:   AE000783
- PIR:   D70202
- RefSeq:   NP_212955.1
- ProteinModelPortal:   O51761
- SMR:   O51761
- EnsemblBacteria:   EBBORT00000008772
- GeneID:   1195679
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0821
- NMPDR:   fig|224326.1.peg.1205
- TIGR:   BB_0821
- GeneTree:   EBGT00050000007306
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   O51761
- ProtClustDB:   PRK14729
- BioCyc:   BBUR224326:BB_0821-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35631; Mature: 35631

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDRVVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKH
CCCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
HLVDFLDPEKDYTIGIFYEQALKIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTS
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEHH
KIRIYVNNLLELKGKSYLLKELKNVDPIRFNMLNKNDIYRIKRSLEVYYQTGIPISQFQK
HHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCHHHHHH
KQSSEFKNIVIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
HHHCCCCEEEEEEECCCHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
YNEFLLWKSRPCYGLNDIIGLINKNSFLYAKRQMTFFAKISDVLWLHPEDDLDNILNLIF
CCEEEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHEEHHHEEEECCCCCHHHHHHHHH
KVDKEI
HHCCCC
>Mature Secondary Structure
MKEDRVVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKH
CCCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
HLVDFLDPEKDYTIGIFYEQALKIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTS
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEHH
KIRIYVNNLLELKGKSYLLKELKNVDPIRFNMLNKNDIYRIKRSLEVYYQTGIPISQFQK
HHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCHHHHHH
KQSSEFKNIVIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
HHHCCCCEEEEEEECCCHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
YNEFLLWKSRPCYGLNDIIGLINKNSFLYAKRQMTFFAKISDVLWLHPEDDLDNILNLIF
CCEEEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHEEHHHEEEECCCCCHHHHHHHHH
KVDKEI
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685