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The map label for this gene is tgt

Identifier: 15595154

GI number: 15595154

Start: 854869

End: 855996

Strand: Direct

Name: tgt

Synonym: BB0809

Alternate gene names: 15595154

Gene position: 854869-855996 (Clockwise)

Preceding gene: 15595153

Following gene: 15595155

Centisome position: 93.87

GC content: 31.38

Gene sequence:

>1128_bases
ATGTTTAGTGTAATCAAGAATGATAAGCATTTTAATGCAAGGGTTGGATTTTTAAATCTTCCTCATGGTAGAGTAGATAT
TCCTTGTTTTATGCCAGTTGGTACTTTGGGGGCAATGAAAGGGTTAAAACATGCTGTTCTTGAGAAGTTAGAATGTAATT
TGATGCTTGCAAATACTTATCATTTATATTTAAGGCTGGGAATTAAAACTGTTGAAAAATATGTTGGTCTTCATAATTTT
ACAATTTGGAATAAAAATTTTTTAACCGATTCGGGCGGATTTCGGGTGTTTTCTTTTTCTGATCTGAGAAAAATTGATCT
AAAAGGTGTGCATTTTAAATCTCATATAGATGGATCGTACCATTATTTTACTTCCGAGGGAATATTTGCTATGCAAGAAA
TTTTTGGCAGTGATATTATTATGCCACTTGACATTTGCAGTTCTTATGGGATTGATTATAATGAAGCCAATTTATATACA
AATATTACAACCAATTGGGCTAGCAGCACGTTCAAATCTTCTAAAAACAGAAAAGAGGGATACAACGGGCTTTTATTTTT
AATAACTCAAGGAAATTTTTTTAAAGATTTAAGGAAAAGAAGTATCAATGATATATTGGAATTAGACAGCCCAGGTATTG
CCATTGGAGGCATTTCTGTTGGAGAACCAAGGGAGAAATATTTAGAAATCCTTGAATATAGTTTTTTGTTGATACCAAAA
GAAAAACCAAGGTATGTAATGGGTATTGGCACTCCCCATTACATACTTAATGCCATATATTATGGCATTGATATTTTTGA
TTGCTTTAATCCCGCAAGAATTACTAGGCATGGGTCTCTTTTGACAGATAATGGGATTATGTGCATTGGTAGAAAGGAGT
ATAAGGATGATACTTCTAAGGTAGAGAAAAATTGCATCTGTACTTTATGTAAAAGGTATTCAAGAGGATATTTAAGACAT
TTAATAAAATCGAAAGAGCTTTTTGGAATAGTTTTGGCAAGTGAACATAATATCCACTATATGTTTCGATTGATTTCAAA
GATCAGAGCCGCAATTCTAAATGATGATTTTTTAAATTTTAGAACTTCATATTTAAAAAAGTATGAAGAGGAAAATTTCG
ATGAATAA

Upstream 100 bases:

>100_bases
ATATAGCAAGTTCTTTTACAACTATATTTTTTTTGATTATCAATTTTTTTGTTTATAGGATTGTTAGGAAATAATATATA
TAATTATTTGGGATATTTAT

Downstream 100 bases:

>100_bases
ATATGTTGTTTCTACAATTTTGGTCATGATTTCCACTTTTTTTTCAAGAATAATGGGCTTTGTAAAGATAAAGATTTTCT
CTTATTATTTTGGTGCAAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFSVIKNDKHFNARVGFLNLPHGRVDIPCFMPVGTLGAMKGLKHAVLEKLECNLMLANTYHLYLRLGIKTVEKYVGLHNF
TIWNKNFLTDSGGFRVFSFSDLRKIDLKGVHFKSHIDGSYHYFTSEGIFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWASSTFKSSKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSFLLIPK
EKPRYVMGIGTPHYILNAIYYGIDIFDCFNPARITRHGSLLTDNGIMCIGRKEYKDDTSKVEKNCICTLCKRYSRGYLRH
LIKSKELFGIVLASEHNIHYMFRLISKIRAAILNDDFLNFRTSYLKKYEEENFDE

Sequences:

>Translated_375_residues
MFSVIKNDKHFNARVGFLNLPHGRVDIPCFMPVGTLGAMKGLKHAVLEKLECNLMLANTYHLYLRLGIKTVEKYVGLHNF
TIWNKNFLTDSGGFRVFSFSDLRKIDLKGVHFKSHIDGSYHYFTSEGIFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWASSTFKSSKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSFLLIPK
EKPRYVMGIGTPHYILNAIYYGIDIFDCFNPARITRHGSLLTDNGIMCIGRKEYKDDTSKVEKNCICTLCKRYSRGYLRH
LIKSKELFGIVLASEHNIHYMFRLISKIRAAILNDDFLNFRTSYLKKYEEENFDE
>Mature_375_residues
MFSVIKNDKHFNARVGFLNLPHGRVDIPCFMPVGTLGAMKGLKHAVLEKLECNLMLANTYHLYLRLGIKTVEKYVGLHNF
TIWNKNFLTDSGGFRVFSFSDLRKIDLKGVHFKSHIDGSYHYFTSEGIFAMQEIFGSDIIMPLDICSSYGIDYNEANLYT
NITTNWASSTFKSSKNRKEGYNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSFLLIPK
EKPRYVMGIGTPHYILNAIYYGIDIFDCFNPARITRHGSLLTDNGIMCIGRKEYKDDTSKVEKNCICTLCKRYSRGYLRH
LIKSKELFGIVLASEHNIHYMFRLISKIRAAILNDDFLNFRTSYLKKYEEENFDE

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=36.6197183098592, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1786607, Length=361, Percent_Identity=38.5041551246537, Blast_Score=272, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17544680, Length=357, Percent_Identity=35.5742296918768, Blast_Score=224, Evalue=8e-59,
Organism=Caenorhabditis elegans, GI25146502, Length=268, Percent_Identity=23.8805970149254, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=35.6948228882834, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI21355311, Length=139, Percent_Identity=28.7769784172662, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BORBU (O51749)

Other databases:

- EMBL:   AE000783
- PIR:   H70200
- RefSeq:   NP_212943.1
- ProteinModelPortal:   O51749
- EnsemblBacteria:   EBBORT00000008789
- GeneID:   1195667
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0809
- NMPDR:   fig|224326.1.peg.1193
- TIGR:   BB_0809
- GeneTree:   EBGT00050000007233
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   BBUR224326:BB_0809-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43199; Mature: 43199

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: ACT_SITE 90-90 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVIKNDKHFNARVGFLNLPHGRVDIPCFMPVGTLGAMKGLKHAVLEKLECNLMLANTY
CCCCCCCCCCCEEEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
HLYLRLGIKTVEKYVGLHNFTIWNKNFLTDSGGFRVFSFSDLRKIDLKGVHFKSHIDGSY
EEEEEHHHHHHHHHHCCCEEEEECCCEEECCCCEEEEEECCCEEECCCCEEEECCCCCCE
HYFTSEGIFAMQEIFGSDIIMPLDICSSYGIDYNEANLYTNITTNWASSTFKSSKNRKEG
EEEECCCHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCC
YNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSFLLIPK
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHEEEEEEC
EKPRYVMGIGTPHYILNAIYYGIDIFDCFNPARITRHGSLLTDNGIMCIGRKEYKDDTSK
CCCCEEEECCCHHHHHHHHHHCCCHHHCCCCHHEEECCCEEECCCEEEEECHHHCCHHHH
VEKNCICTLCKRYSRGYLRHLIKSKELFGIVLASEHNIHYMFRLISKIRAAILNDDFLNF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHH
RTSYLKKYEEENFDE
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MFSVIKNDKHFNARVGFLNLPHGRVDIPCFMPVGTLGAMKGLKHAVLEKLECNLMLANTY
CCCCCCCCCCCEEEEEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
HLYLRLGIKTVEKYVGLHNFTIWNKNFLTDSGGFRVFSFSDLRKIDLKGVHFKSHIDGSY
EEEEEHHHHHHHHHHCCCEEEEECCCEEECCCCEEEEEECCCEEECCCCEEEECCCCCCE
HYFTSEGIFAMQEIFGSDIIMPLDICSSYGIDYNEANLYTNITTNWASSTFKSSKNRKEG
EEEECCCHHHHHHHHCCCCEEEHHHHHHCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCC
YNGLLFLITQGNFFKDLRKRSINDILELDSPGIAIGGISVGEPREKYLEILEYSFLLIPK
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHEEEEEEC
EKPRYVMGIGTPHYILNAIYYGIDIFDCFNPARITRHGSLLTDNGIMCIGRKEYKDDTSK
CCCCEEEECCCHHHHHHHHHHCCCHHHCCCCHHEEECCCEEECCCEEEEECHHHCCHHHH
VEKNCICTLCKRYSRGYLRHLIKSKELFGIVLASEHNIHYMFRLISKIRAAILNDDFLNF
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHH
RTSYLKKYEEENFDE
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685