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The map label for this gene is pnp [H]

Identifier: 15595150

GI number: 15595150

Start: 848819

End: 850987

Strand: Direct

Name: pnp [H]

Synonym: BB0805

Alternate gene names: 15595150

Gene position: 848819-850987 (Clockwise)

Preceding gene: 15595149

Following gene: 15595151

Centisome position: 93.2

GC content: 35.41

Gene sequence:

>2169_bases
TTGAGGAAAATATTAAAGTTGAAAATAGGCAGAGACGAGTTAGTGTTTGAGACCGGATTTATGGCTAAACAGGCTAATGG
ATCGGTTCTTGCAACTTATGGTGGATCTTCGGTTCTTGCAACTGTTTGTTGCTCGAGTAACGTGAGAGAAGATTTAGATT
TTGTTCCGCTTTCTGTTGAATATAATGAGAAATATTATGCAGCCGGTAAAATTCCGGGAGGATTTATCAAAAGAGAAGGA
AAGCCAAAGGATAAAGAAATACTTGTTTCCAGACTAATAGACAGGCCGATGAGACCTCTTTTTGATAAAAGATTTGGTCG
AGAAATTCAAGTAATTCCTACAACTTTAGCTACAGATCAGCTTAATCCTCCTGATATTGTTGGAATGAATGCTGCTTTTA
CGGCAGTTTTTTTGTCAGATATTCCGTTTAATGGTCCAATTGCAGCTGTTAGAATGGTTTATTTGAATGGTAAGTTTATA
GTAAACCCTTCGTTTGAAGAGATTCATGATTCTGATCTTGATATTGTTGTTGCCGGAAGTTTAAATGGAATTACTATGGT
AGAAGGTGGTGCTAATGAGGTTGGTGAGGATATTTTGCTCTCAGCAATAGATGGTGCTCATGAATATATTAAGCAAATTT
GTAATGCTCAAAAAGAATTTTTAGATATTGTAGGCAAGAAGGAAAAACTTCCTTTAGCTTTTGAAGAAAAAATATTTGAA
TTTAAAGATGAGCTTAGGGATTTTGTTTATGCTGATCTTAAAGAAGCTTGTTTTGTTAAGGGAAAGCTTAATAGAGATAA
AGCCATAACTTTGCTGCGAAATAAATCTTATGAGTACTTTTCTTCTCTTGAGAAATTGACTGATAGCAATGAATCTCTTT
TTCATAAGGCTTTTGATGATTTTGAGAAGGAGATTGTTAGAAGCTCTATTCTTAACGATAATATTAGAACAGATGGCAGA
ACTCCTAATGAGATAAGAGATATTATCTCAGAAGTTGATATTTTAAGTAGAACGCATGGATCTGCGCTTTTTACAAGGGG
AGAGACGCAAGCTTTAGCGGTAACTACTCTTGGTACAAGCATTGATGAACAAATAATGGATGATATTGATGGCGATAAAC
GCCTTAATTTTATGCTCCACTACAATTTTCCCCCATTTTCAGTTGGTGAGACCGGTAGACTAATGACTGGCAGGCGCGAG
ATTGGCCATGGTCATTTAGCTCAAAGAGCTTTAGAATCAATGGTTCCTGGAAAAAATGATTTTCCTTATACCATTAGGGT
AGTTTCTGAGGTTTTAGAGTCTAACGGATCTTCTTCAATGGCTACTGTTTGTGCTGGGAGCATGTCTTTAATGTCAGCAG
GGGTTCCTGTTAAAGGGCAGGTTGCGGGGATAGCCATGGGGCTTATTAGCGAAGGGGATAAATATGTAGTTTTAAGTGAT
ATTCTTGGAGAAGAAGATCATCTAGGTGATATGGACTTTAAAGTGGCCGGTACAAAAAATGGAATTACTGGATTTCAAAT
GGATATTAAGATTGAAAATGTTACTAAAGATTTAATGAGAGATGCTCTTGAGCAAGCAAGAATAGGTAGAATACATATAT
TATCTATTATGAATACTGTAATTTCTAATTCAAGAGTTGGTATATCTAAGTATGCTCCTAAAATTGTTCAACTTCAAATT
GACATTGACAAGATATCTCTTGTGATAGGATCTACTGGAAAAACTGTTAAGGCTATAACAGATGAATTTGAAGTTAAGGT
TCAAATTGAGCAGAATGGAAAAATCATTCTTTTCGGGGATGATGATTTTAAGATGCAAAAAGCTAAAGAAAGAATAGAGA
GTATTGTAAGAGAGCCAAAGGTAGGCGAAATTTATGAAGGAACTGTCAAAAAGATTAATAGCTTTGGGGCTTTTATTGAA
CTTACTCCTGCAAAGGAAGGATTTTTAAGTACTCGTTTGAAACCCAGAGACAGTAAGTATGGTTCTGGAAGATTTGGGAA
TAGTAATAGATATTCTAGATTTGGTGGCGGTGGAGAGAATATAAGAGGTAATGCGGGGTTAGTTCGACCCCCAAAATTAG
AAGAAGGTCAGCGAATTAAGGTCAAAATAATTGATATAGACAAGTTTGGAAAAATTGATCTTGAAATTGTTAGAGATAAA
GATTATTAA

Upstream 100 bases:

>100_bases
AAAGGCGAAGTTTATTGCGGTATTACCAGAAAAAAGATTTAGAAGCTTATAGGATGTTGATATCTAAACTTGGTCTTAGA
AAATAAAAAGAGGTTGAATT

Downstream 100 bases:

>100_bases
AATAATATGAAATTTGTTTTGAATAATTTATTTAAAGGTTGTCTTATATGTTTTTTCTTGTTTTTTTCCTGCCTTACTAC
AGATAGATCTATTCAAGATT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MRKILKLKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSSNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREG
KPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFI
VNPSFEEIHDSDLDIVVAGSLNGITMVEGGANEVGEDILLSAIDGAHEYIKQICNAQKEFLDIVGKKEKLPLAFEEKIFE
FKDELRDFVYADLKEACFVKGKLNRDKAITLLRNKSYEYFSSLEKLTDSNESLFHKAFDDFEKEIVRSSILNDNIRTDGR
TPNEIRDIISEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRRE
IGHGHLAQRALESMVPGKNDFPYTIRVVSEVLESNGSSSMATVCAGSMSLMSAGVPVKGQVAGIAMGLISEGDKYVVLSD
ILGEEDHLGDMDFKVAGTKNGITGFQMDIKIENVTKDLMRDALEQARIGRIHILSIMNTVISNSRVGISKYAPKIVQLQI
DIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGTVKKINSFGAFIE
LTPAKEGFLSTRLKPRDSKYGSGRFGNSNRYSRFGGGGENIRGNAGLVRPPKLEEGQRIKVKIIDIDKFGKIDLEIVRDK
DY

Sequences:

>Translated_722_residues
MRKILKLKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSSNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREG
KPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFI
VNPSFEEIHDSDLDIVVAGSLNGITMVEGGANEVGEDILLSAIDGAHEYIKQICNAQKEFLDIVGKKEKLPLAFEEKIFE
FKDELRDFVYADLKEACFVKGKLNRDKAITLLRNKSYEYFSSLEKLTDSNESLFHKAFDDFEKEIVRSSILNDNIRTDGR
TPNEIRDIISEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRRE
IGHGHLAQRALESMVPGKNDFPYTIRVVSEVLESNGSSSMATVCAGSMSLMSAGVPVKGQVAGIAMGLISEGDKYVVLSD
ILGEEDHLGDMDFKVAGTKNGITGFQMDIKIENVTKDLMRDALEQARIGRIHILSIMNTVISNSRVGISKYAPKIVQLQI
DIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGTVKKINSFGAFIE
LTPAKEGFLSTRLKPRDSKYGSGRFGNSNRYSRFGGGGENIRGNAGLVRPPKLEEGQRIKVKIIDIDKFGKIDLEIVRDK
DY
>Mature_722_residues
MRKILKLKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSSNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREG
KPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFI
VNPSFEEIHDSDLDIVVAGSLNGITMVEGGANEVGEDILLSAIDGAHEYIKQICNAQKEFLDIVGKKEKLPLAFEEKIFE
FKDELRDFVYADLKEACFVKGKLNRDKAITLLRNKSYEYFSSLEKLTDSNESLFHKAFDDFEKEIVRSSILNDNIRTDGR
TPNEIRDIISEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRRE
IGHGHLAQRALESMVPGKNDFPYTIRVVSEVLESNGSSSMATVCAGSMSLMSAGVPVKGQVAGIAMGLISEGDKYVVLSD
ILGEEDHLGDMDFKVAGTKNGITGFQMDIKIENVTKDLMRDALEQARIGRIHILSIMNTVISNSRVGISKYAPKIVQLQI
DIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGTVKKINSFGAFIE
LTPAKEGFLSTRLKPRDSKYGSGRFGNSNRYSRFGGGGENIRGNAGLVRPPKLEEGQRIKVKIIDIDKFGKIDLEIVRDK
DY

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=688, Percent_Identity=35.6104651162791, Blast_Score=411, Evalue=1e-114,
Organism=Escherichia coli, GI145693187, Length=718, Percent_Identity=42.2005571030641, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI115534063, Length=653, Percent_Identity=33.9969372128637, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI281362905, Length=661, Percent_Identity=36.4599092284418, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24651641, Length=661, Percent_Identity=36.4599092284418, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24651643, Length=661, Percent_Identity=36.4599092284418, Blast_Score=396, Evalue=1e-110,
Organism=Drosophila melanogaster, GI161079377, Length=605, Percent_Identity=37.1900826446281, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 80064; Mature: 80064

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILKLKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSSNVREDLDFVPLSVE
CCCEEEEEECCCCEEEECCCCEECCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEEEE
YNEKYYAAGKIPGGFIKREGKPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQ
ECCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEECCC
LNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFEEIHDSDLDIVVAGS
CCCCCEEECCHHHEEHHHHCCCCCCCHHEEEEEEECCEEEECCCHHHHCCCCCCEEEECC
LNGITMVEGGANEVGEDILLSAIDGAHEYIKQICNAQKEFLDIVGKKEKLPLAFEEKIFE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FKDELRDFVYADLKEACFVKGKLNRDKAITLLRNKSYEYFSSLEKLTDSNESLFHKAFDD
HHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
FEKEIVRSSILNDNIRTDGRTPNEIRDIISEVDILSRTHGSALFTRGETQALAVTTLGTS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCC
IDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHLAQRALESMVPGKND
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCHHHHHHHHHHHCCCCCC
FPYTIRVVSEVLESNGSSSMATVCAGSMSLMSAGVPVKGQVAGIAMGLISEGDKYVVLSD
CCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCCCHHHEEEHHHCCCCCEEEEHH
ILGEEDHLGDMDFKVAGTKNGITGFQMDIKIENVTKDLMRDALEQARIGRIHILSIMNTV
HCCCCCCCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ISNSRVGISKYAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGD
HCCCCCCHHHHCCEEEEEEEEHEEEEEEECCCCCEEHEECCCEEEEEEEECCCEEEEECC
DDFKMQKAKERIESIVREPKVGEIYEGTVKKINSFGAFIELTPAKEGFLSTRLKPRDSKY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCCCC
GSGRFGNSNRYSRFGGGGENIRGNAGLVRPPKLEEGQRIKVKIIDIDKFGKIDLEIVRDK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECC
DY
CC
>Mature Secondary Structure
MRKILKLKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSSNVREDLDFVPLSVE
CCCEEEEEECCCCEEEECCCCEECCCCCEEEEECCCHHHHHHHHCCCCCCCCCEEEEEEE
YNEKYYAAGKIPGGFIKREGKPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQ
ECCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEECCC
LNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFEEIHDSDLDIVVAGS
CCCCCEEECCHHHEEHHHHCCCCCCCHHEEEEEEECCEEEECCCHHHHCCCCCCEEEECC
LNGITMVEGGANEVGEDILLSAIDGAHEYIKQICNAQKEFLDIVGKKEKLPLAFEEKIFE
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
FKDELRDFVYADLKEACFVKGKLNRDKAITLLRNKSYEYFSSLEKLTDSNESLFHKAFDD
HHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHH
FEKEIVRSSILNDNIRTDGRTPNEIRDIISEVDILSRTHGSALFTRGETQALAVTTLGTS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCC
IDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHLAQRALESMVPGKND
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCHHHHHHHHHHHCCCCCC
FPYTIRVVSEVLESNGSSSMATVCAGSMSLMSAGVPVKGQVAGIAMGLISEGDKYVVLSD
CCCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCCCHHHEEEHHHCCCCCEEEEHH
ILGEEDHLGDMDFKVAGTKNGITGFQMDIKIENVTKDLMRDALEQARIGRIHILSIMNTV
HCCCCCCCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ISNSRVGISKYAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGD
HCCCCCCHHHHCCEEEEEEEEHEEEEEEECCCCCEEHEECCCEEEEEEEECCCEEEEECC
DDFKMQKAKERIESIVREPKVGEIYEGTVKKINSFGAFIELTPAKEGFLSTRLKPRDSKY
CCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHCCCCCCCCCC
GSGRFGNSNRYSRFGGGGENIRGNAGLVRPPKLEEGQRIKVKIIDIDKFGKIDLEIVRDK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEEECC
DY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA