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The map label for this gene is fusA2 [H]

Identifier: 15595036

GI number: 15595036

Start: 731794

End: 733803

Strand: Direct

Name: fusA2 [H]

Synonym: BB0691

Alternate gene names: 15595036

Gene position: 731794-733803 (Clockwise)

Preceding gene: 15595035

Following gene: 15595037

Centisome position: 80.35

GC content: 30.65

Gene sequence:

>2010_bases
ATGAGTATTAGAAATATTGGAATTATGGCTCATATTGACGCTGGAAAAACTACTACCACAGAAAGAATTATTTATTATAC
TGGCAAAAGTCATAAAATGGGGGATGTAGATTCAGGAAACACTATTACTGACTGGATGCCTCAAGAGCAAGAAAGAGGAA
TTACTATTAGTTCAGCTGCCATTACTTGTCATTGGAAGGATTGCCAAATAAACATTATTGATACTCCTGGGCATGTGGAT
TTTACAGCAGAAGTTGAAAGATCTCTTCGAGTTCTTGATGGGGGTATTGTTATTTTTAGCGCTGTTGATGGAATTCAGGC
CCAAACAGAAACTGTATGGAAACAGGCAGAAAAATACGAAATCCCACGACTTGCTTATGTTAATAAGATGGATAGAATAG
GTGCTGATTTTTTTAAAGTTGTGGGAGATATTGAAAATAAATTTAAAACTATTCCTTTGGTTTTGCAAATTCCAATTGGA
AATGAAAGCAATTTTGAAGGAGTAGTTGATATTATTTTAAATAAAGAGCTTCATTTTTCAATGGAAAATGGAATTCCAAA
ATTAACTTATAGTCAAATTAGAGAAGAATTTATTGAAAAAGTGATTCTTTTTAAAAAAAAATTAATAGACATTCTTAGCC
AATTTAGTGAAGAAATTACTCAATTATTTCTTGAAGACAAAGAGATTGGTTTAGATATTATTAAAAGAGAGATTAGAAGA
GGCACTATTTCTAGATTTATTATTCCTGTTTTAATGGGAACTAGTTTAAAAAATATTGGAATAGAACCTTTGATTGATTC
GATTGTAGATTACTTGCCAAGTCCTTTTGAAAAAAGTTTTAGCGCTTTTTCTTTAGACACAAATAAAAAAATTTTAGTTG
ATCCTAATGAAAATAAAAAATTGTCAGCCCTTGTTTTTAAAGTTCAATATTCAAGCGTGATTGCTTCTCATCTTTATTTT
GTTAGAGTTTATTCTGGCGAGATTAATCCTAATAAAAAAATTATCAATGCTTCAAATGGTAAGCGTGAAAAGTTTACAAA
AATTTTTAGAGTTTTTTCAAATAAAAATGAACAAATAGATTTTGTAAAAACAGGCGATATTGGTGCTGTTTTGGGATTAA
AGTTTTCTGTTACAGGAGATACTCTTGTTGAAGAGAATAATAATGTTTTACTTGAGGCTGTTATGTTTCCAGAGCCGGTT
GTTTTAATGTCTGTTGAGCCTGAAAGATCATCAGATGAGGTTAGGCTTAAGGAAATTTTTGAAATAATATCTAAAGAAGA
TCCTACCTTTAGTTATTCTGAGAGTAAAGAAACAGGGCAATTAATTATATCTGGAATGGGTGAATTACATCTTGAGATTA
TTTTAACAAGAATTAAAGATGAATTTAATCTTAATGTTTATACAGGAAAGCCTCAAGTAAGTTACAGAGAAAGTGCTGGC
AAAATTGTAAAAGAAGTTTTTGAGTTTAACAATATTTTTGCTGGCAAAAATATTGATTTTAAAATTGGAATGATCATTAA
ACCTTTGTCGCGGGGTGCGGGAAATAAAATTGATTTTGAATGTGGCATTGAACCTGTAATTAAGTCTGCAATATTGAGGG
GAATTACATCTGCATTTGTAAGTGGAATTTTTGGATATCCCATTATTGATATTAATGTTAGTATTTTTTCTATTGTTTGT
GGGGCCAATAAGATTAGCGAGAGTGCTTTTGAGTCAATTTCAGGATTTGCTTTTCATAGTATTTTTCAAAAATCAGATCC
TATTAGACTTGAGCCAATAATGTTATTAGAAATTAGAACACCCATTGAGCATACAGGCGAAATTATTTCTACATTAAATG
TTATGGGGGGTGTTATTCATTCAGTTAGCAATATTGGAGAGTATGACTTGATAAAATCAGAGGCGGCGTTTGAGAAACTT
TTTGGGTATGCTTCTATTTTGAGAAGTTCTACTAAAGGAAGGGGCAGTTTTACTATGGAATTTTCTTATTTTAAGGAAAA
ATTAAGTTAA

Upstream 100 bases:

>100_bases
AAATGTTGCGAGTGTGATGCAAAATAGCAATTTTATTAAAAGGTTTAACCTGTGCTAATGAATTTATCTGCATTGAGGCT
GTGGTAGTTGTTGGGGATTT

Downstream 100 bases:

>100_bases
AGTTAGTGGTTTTTAAGGCAAAATGGCTTTTCTTGTATTTATTATTTTTAAATCCAATTAATTTTATTGATGAGTTTTTA
TGTAAAAATGATTTTATTCC

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 2 [H]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSIRNIGIMAHIDAGKTTTTERIIYYTGKSHKMGDVDSGNTITDWMPQEQERGITISSAAITCHWKDCQINIIDTPGHVD
FTAEVERSLRVLDGGIVIFSAVDGIQAQTETVWKQAEKYEIPRLAYVNKMDRIGADFFKVVGDIENKFKTIPLVLQIPIG
NESNFEGVVDIILNKELHFSMENGIPKLTYSQIREEFIEKVILFKKKLIDILSQFSEEITQLFLEDKEIGLDIIKREIRR
GTISRFIIPVLMGTSLKNIGIEPLIDSIVDYLPSPFEKSFSAFSLDTNKKILVDPNENKKLSALVFKVQYSSVIASHLYF
VRVYSGEINPNKKIINASNGKREKFTKIFRVFSNKNEQIDFVKTGDIGAVLGLKFSVTGDTLVEENNNVLLEAVMFPEPV
VLMSVEPERSSDEVRLKEIFEIISKEDPTFSYSESKETGQLIISGMGELHLEIILTRIKDEFNLNVYTGKPQVSYRESAG
KIVKEVFEFNNIFAGKNIDFKIGMIIKPLSRGAGNKIDFECGIEPVIKSAILRGITSAFVSGIFGYPIIDINVSIFSIVC
GANKISESAFESISGFAFHSIFQKSDPIRLEPIMLLEIRTPIEHTGEIISTLNVMGGVIHSVSNIGEYDLIKSEAAFEKL
FGYASILRSSTKGRGSFTMEFSYFKEKLS

Sequences:

>Translated_669_residues
MSIRNIGIMAHIDAGKTTTTERIIYYTGKSHKMGDVDSGNTITDWMPQEQERGITISSAAITCHWKDCQINIIDTPGHVD
FTAEVERSLRVLDGGIVIFSAVDGIQAQTETVWKQAEKYEIPRLAYVNKMDRIGADFFKVVGDIENKFKTIPLVLQIPIG
NESNFEGVVDIILNKELHFSMENGIPKLTYSQIREEFIEKVILFKKKLIDILSQFSEEITQLFLEDKEIGLDIIKREIRR
GTISRFIIPVLMGTSLKNIGIEPLIDSIVDYLPSPFEKSFSAFSLDTNKKILVDPNENKKLSALVFKVQYSSVIASHLYF
VRVYSGEINPNKKIINASNGKREKFTKIFRVFSNKNEQIDFVKTGDIGAVLGLKFSVTGDTLVEENNNVLLEAVMFPEPV
VLMSVEPERSSDEVRLKEIFEIISKEDPTFSYSESKETGQLIISGMGELHLEIILTRIKDEFNLNVYTGKPQVSYRESAG
KIVKEVFEFNNIFAGKNIDFKIGMIIKPLSRGAGNKIDFECGIEPVIKSAILRGITSAFVSGIFGYPIIDINVSIFSIVC
GANKISESAFESISGFAFHSIFQKSDPIRLEPIMLLEIRTPIEHTGEIISTLNVMGGVIHSVSNIGEYDLIKSEAAFEKL
FGYASILRSSTKGRGSFTMEFSYFKEKLS
>Mature_668_residues
SIRNIGIMAHIDAGKTTTTERIIYYTGKSHKMGDVDSGNTITDWMPQEQERGITISSAAITCHWKDCQINIIDTPGHVDF
TAEVERSLRVLDGGIVIFSAVDGIQAQTETVWKQAEKYEIPRLAYVNKMDRIGADFFKVVGDIENKFKTIPLVLQIPIGN
ESNFEGVVDIILNKELHFSMENGIPKLTYSQIREEFIEKVILFKKKLIDILSQFSEEITQLFLEDKEIGLDIIKREIRRG
TISRFIIPVLMGTSLKNIGIEPLIDSIVDYLPSPFEKSFSAFSLDTNKKILVDPNENKKLSALVFKVQYSSVIASHLYFV
RVYSGEINPNKKIINASNGKREKFTKIFRVFSNKNEQIDFVKTGDIGAVLGLKFSVTGDTLVEENNNVLLEAVMFPEPVV
LMSVEPERSSDEVRLKEIFEIISKEDPTFSYSESKETGQLIISGMGELHLEIILTRIKDEFNLNVYTGKPQVSYRESAGK
IVKEVFEFNNIFAGKNIDFKIGMIIKPLSRGAGNKIDFECGIEPVIKSAILRGITSAFVSGIFGYPIIDINVSIFSIVCG
ANKISESAFESISGFAFHSIFQKSDPIRLEPIMLLEIRTPIEHTGEIISTLNVMGGVIHSVSNIGEYDLIKSEAAFEKLF
GYASILRSSTKGRGSFTMEFSYFKEKLS

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=707, Percent_Identity=35.0777934936351, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI18390331, Length=686, Percent_Identity=33.9650145772595, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI25306283, Length=447, Percent_Identity=40.2684563758389, Blast_Score=308, Evalue=7e-84,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=47.9310344827586, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI94966754, Length=138, Percent_Identity=39.8550724637681, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=38.9261744966443, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI157426893, Length=215, Percent_Identity=31.1627906976744, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI310132016, Length=119, Percent_Identity=38.655462184874, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310110807, Length=119, Percent_Identity=38.655462184874, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI310123363, Length=119, Percent_Identity=38.655462184874, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=33.8235294117647, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=33.8235294117647, Blast_Score=77, Evalue=7e-14,
Organism=Homo sapiens, GI94966752, Length=64, Percent_Identity=45.3125, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789738, Length=683, Percent_Identity=40.5563689604685, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1790835, Length=475, Percent_Identity=23.1578947368421, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=133, Percent_Identity=39.0977443609023, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=150, Percent_Identity=38.6666666666667, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=686, Percent_Identity=34.9854227405248, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=47.2527472527472, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17557151, Length=176, Percent_Identity=37.5, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=37.2670807453416, Blast_Score=96, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.0827067669173, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17552882, Length=136, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=694, Percent_Identity=35.8789625360231, Blast_Score=425, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322359, Length=776, Percent_Identity=31.1855670103093, Blast_Score=352, Evalue=8e-98,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.255033557047, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.255033557047, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323320, Length=152, Percent_Identity=34.8684210526316, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324166, Length=148, Percent_Identity=33.1081081081081, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=130, Percent_Identity=31.5384615384615, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=691, Percent_Identity=35.6005788712012, Blast_Score=418, Evalue=1e-117,
Organism=Drosophila melanogaster, GI221458488, Length=710, Percent_Identity=32.112676056338, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24585709, Length=152, Percent_Identity=39.4736842105263, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585711, Length=152, Percent_Identity=39.4736842105263, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585713, Length=152, Percent_Identity=39.4736842105263, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI78706572, Length=154, Percent_Identity=36.3636363636364, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357743, Length=596, Percent_Identity=20.9731543624161, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28574573, Length=209, Percent_Identity=29.6650717703349, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75058; Mature: 74926

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRNIGIMAHIDAGKTTTTERIIYYTGKSHKMGDVDSGNTITDWMPQEQERGITISSAA
CCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCHHHCCEEEEEEE
ITCHWKDCQINIIDTPGHVDFTAEVERSLRVLDGGIVIFSAVDGIQAQTETVWKQAEKYE
EEEEECCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC
IPRLAYVNKMDRIGADFFKVVGDIENKFKTIPLVLQIPIGNESNFEGVVDIILNKELHFS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHEEECCCEEEE
MENGIPKLTYSQIREEFIEKVILFKKKLIDILSQFSEEITQLFLEDKEIGLDIIKREIRR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHH
GTISRFIIPVLMGTSLKNIGIEPLIDSIVDYLPSPFEKSFSAFSLDTNKKILVDPNENKK
HHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCHHHCCCEEEECCCCEEEECCCCCCE
LSALVFKVQYSSVIASHLYFVRVYSGEINPNKKIINASNGKREKFTKIFRVFSNKNEQID
EEEEEEEEHHHHHHHHHEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEE
FVKTGDIGAVLGLKFSVTGDTLVEENNNVLLEAVMFPEPVVLMSVEPERSSDEVRLKEIF
EEEECCCEEEEEEEEEECCCEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHH
EIISKEDPTFSYSESKETGQLIISGMGELHLEIILTRIKDEFNLNVYTGKPQVSYRESAG
HHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
KIVKEVFEFNNIFAGKNIDFKIGMIIKPLSRGAGNKIDFECGIEPVIKSAILRGITSAFV
HHHHHHHHHHCEECCCCCCEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
SGIFGYPIIDINVSIFSIVCGANKISESAFESISGFAFHSIFQKSDPIRLEPIMLLEIRT
HHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECC
PIEHTGEIISTLNVMGGVIHSVSNIGEYDLIKSEAAFEKLFGYASILRSSTKGRGSFTME
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FSYFKEKLS
HHHHHHHCC
>Mature Secondary Structure 
SIRNIGIMAHIDAGKTTTTERIIYYTGKSHKMGDVDSGNTITDWMPQEQERGITISSAA
CCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEECCCCCHHHCCEEEEEEE
ITCHWKDCQINIIDTPGHVDFTAEVERSLRVLDGGIVIFSAVDGIQAQTETVWKQAEKYE
EEEEECCCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCC
IPRLAYVNKMDRIGADFFKVVGDIENKFKTIPLVLQIPIGNESNFEGVVDIILNKELHFS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCHHHHEEECCCEEEE
MENGIPKLTYSQIREEFIEKVILFKKKLIDILSQFSEEITQLFLEDKEIGLDIIKREIRR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHH
GTISRFIIPVLMGTSLKNIGIEPLIDSIVDYLPSPFEKSFSAFSLDTNKKILVDPNENKK
HHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCHHHCCCEEEECCCCEEEECCCCCCE
LSALVFKVQYSSVIASHLYFVRVYSGEINPNKKIINASNGKREKFTKIFRVFSNKNEQID
EEEEEEEEHHHHHHHHHEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCEEE
FVKTGDIGAVLGLKFSVTGDTLVEENNNVLLEAVMFPEPVVLMSVEPERSSDEVRLKEIF
EEEECCCEEEEEEEEEECCCEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHH
EIISKEDPTFSYSESKETGQLIISGMGELHLEIILTRIKDEFNLNVYTGKPQVSYRESAG
HHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
KIVKEVFEFNNIFAGKNIDFKIGMIIKPLSRGAGNKIDFECGIEPVIKSAILRGITSAFV
HHHHHHHHHHCEECCCCCCEEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
SGIFGYPIIDINVSIFSIVCGANKISESAFESISGFAFHSIFQKSDPIRLEPIMLLEIRT
HHHCCCEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECC
PIEHTGEIISTLNVMGGVIHSVSNIGEYDLIKSEAAFEKLFGYASILRSSTKGRGSFTME
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FSYFKEKLS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA