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The map label for this gene is amiB [H]

Identifier: 15595011

GI number: 15595011

Start: 703931

End: 704959

Strand: Direct

Name: amiB [H]

Synonym: BB0666

Alternate gene names: 15595011

Gene position: 703931-704959 (Clockwise)

Preceding gene: 15595010

Following gene: 15595012

Centisome position: 77.29

GC content: 27.11

Gene sequence:

>1029_bases
GTGCCATTGAGCTTTGGGAAAATGATATTATTGATTGATTTGATTTTGTTTTCATATTTAAGCTTGTATTCTAAGACCCC
CGATTATTTAAATGTTCTTGATTTTTTTGATACTAATGTTTTTAGGTTTGATTTTAACATTGAAAATGATGTTTTTACAA
TTGAAAATGATAAGGGATATTTGAAGTTTAGGGTAGGCTTTGAATATGCGCTTACATCTTCTGGTTATTATATGTTTGTA
GACCCAATTATTGACATTCGTGGAGAAATTTTAATAAGTCAAAAGGTATTAAAACAAATTGAAAATTATTTTAGTTCTCT
TAAAGACTATAATAAACCCAGAATTACTTCAATAATCATTGATCCTGGACATGGCGGGCATGATACTGGTGCTGTTGTGA
CTTTAAAGATAAATGGTTATGACGTTGTGCTTCAGGAAAAAGATTTTGCATTAACCTATTCTATATATTTGTCTAAAATT
TTAAGTAATTATTTTGTAAATAAAAATATTTTGTTAACTCGTATAAATGATGTTTATTTAACTTTAAAAGAACGGTCGGA
ATTTGCAAATGCAATAAAGCCAAATTTCCCCAATAATGTTATATTTTTATCTATACATGCTAATGATGCTCCAAACGGTG
AAGCTAGAGGAGTTGAGTTTTGGTATCTTCCTAAGGATTCAAAAAGAGAAGTTATTAAAGATTTTAAGGGATATGATATT
AAAGGTAATAGATACTTAAGCGAGCTTAATGATATACTAGATATTAAATATAAATATGAATCAAAAAGATTGGCTGAAAT
TTTGTATAAAGTGTTTAAAAATGAGTTAAGCGAAACTAATATTAGGCCAATCAGAAAAGAGCAATGGTTTGTAATAAAAA
ACAGCAGTATGCCTGCTGTGTTGATTGAAATGGGGTTTTTATCCAATATTTTAGATGCTAAATTAATTTTGGATTATAAT
TACATGAGCAAGTTTAATATTCTAATACTTAAAGCTTTAATGGAATTTATAAATTTTTATGAAAAATAA

Upstream 100 bases:

>100_bases
TTAAAATACAAAATTAATCCTTTTCTCTTCCAAAAAGAGTTTAAGGAGATAAAAGGTGAGATTACTAAAAATAGCTTAAG
ATTCAAAGGAGACTTAAAGA

Downstream 100 bases:

>100_bases
TATTTCGGATATATTTTTTAAATATAATGTAGTGATTTATAAATTTTTAAATTCAAAAAAAGAGCATATGATTAGGGTGC
TTTTGGGGTCTTTGGCAGTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MPLSFGKMILLIDLILFSYLSLYSKTPDYLNVLDFFDTNVFRFDFNIENDVFTIENDKGYLKFRVGFEYALTSSGYYMFV
DPIIDIRGEILISQKVLKQIENYFSSLKDYNKPRITSIIIDPGHGGHDTGAVVTLKINGYDVVLQEKDFALTYSIYLSKI
LSNYFVNKNILLTRINDVYLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNGEARGVEFWYLPKDSKREVIKDFKGYDI
KGNRYLSELNDILDIKYKYESKRLAEILYKVFKNELSETNIRPIRKEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYN
YMSKFNILILKALMEFINFYEK

Sequences:

>Translated_342_residues
MPLSFGKMILLIDLILFSYLSLYSKTPDYLNVLDFFDTNVFRFDFNIENDVFTIENDKGYLKFRVGFEYALTSSGYYMFV
DPIIDIRGEILISQKVLKQIENYFSSLKDYNKPRITSIIIDPGHGGHDTGAVVTLKINGYDVVLQEKDFALTYSIYLSKI
LSNYFVNKNILLTRINDVYLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNGEARGVEFWYLPKDSKREVIKDFKGYDI
KGNRYLSELNDILDIKYKYESKRLAEILYKVFKNELSETNIRPIRKEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYN
YMSKFNILILKALMEFINFYEK
>Mature_341_residues
PLSFGKMILLIDLILFSYLSLYSKTPDYLNVLDFFDTNVFRFDFNIENDVFTIENDKGYLKFRVGFEYALTSSGYYMFVD
PIIDIRGEILISQKVLKQIENYFSSLKDYNKPRITSIIIDPGHGGHDTGAVVTLKINGYDVVLQEKDFALTYSIYLSKIL
SNYFVNKNILLTRINDVYLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNGEARGVEFWYLPKDSKREVIKDFKGYDIK
GNRYLSELNDILDIKYKYESKRLAEILYKVFKNELSETNIRPIRKEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYNY
MSKFNILILKALMEFINFYEK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=237, Percent_Identity=24.4725738396624, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788776, Length=278, Percent_Identity=24.4604316546763, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39972; Mature: 39841

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSFGKMILLIDLILFSYLSLYSKTPDYLNVLDFFDTNVFRFDFNIENDVFTIENDKGY
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECCCCE
LKFRVGFEYALTSSGYYMFVDPIIDIRGEILISQKVLKQIENYFSSLKDYNKPRITSIII
EEEEEEEEEEEECCCEEEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPGHGGHDTGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFVNKNILLTRINDVYL
ECCCCCCCCCEEEEEEECCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCEEEEEECEEEE
TLKERSEFANAIKPNFPNNVIFLSIHANDAPNGEARGVEFWYLPKDSKREVIKDFKGYDI
EEEHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
KGNRYLSELNDILDIKYKYESKRLAEILYKVFKNELSETNIRPIRKEQWFVIKNSSMPAV
CCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEE
LIEMGFLSNILDAKLILDYNYMSKFNILILKALMEFINFYEK
EEHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLSFGKMILLIDLILFSYLSLYSKTPDYLNVLDFFDTNVFRFDFNIENDVFTIENDKGY
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEECCCEEEEECCCCE
LKFRVGFEYALTSSGYYMFVDPIIDIRGEILISQKVLKQIENYFSSLKDYNKPRITSIII
EEEEEEEEEEEECCCEEEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEE
DPGHGGHDTGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFVNKNILLTRINDVYL
ECCCCCCCCCEEEEEEECCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCEEEEEECEEEE
TLKERSEFANAIKPNFPNNVIFLSIHANDAPNGEARGVEFWYLPKDSKREVIKDFKGYDI
EEEHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
KGNRYLSELNDILDIKYKYESKRLAEILYKVFKNELSETNIRPIRKEQWFVIKNSSMPAV
CCCHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEE
LIEMGFLSNILDAKLILDYNYMSKFNILILKALMEFINFYEK
EEHHHHHHHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]